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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #28838844 -

Made popcorn jars with LC. LC was purchased from a reputable source and all jar's inoculation was done in a SAB.
The jars were looking really great all 4 of them(sadly didn't take pictures ),no discoloured part  or anything like that. Pure white mycelium growth,i even had to shake and hit the jar itself to break up them up when i was mixing. So i'm not really sure what happened considering i mixed things in a SAB both times.
I suspect the liner itself was not clean enough.

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Re: Ask Quick Questions, Get Quick Answers [Re: kimura999]
    #28838901 -

towerofbabel said:
Hey everyone! I know the general consensus is to not use pre-made bags, but this particular brand I've had success with. I'm 2 for 2 so far. This is my third, and I figure if it keeps working, why try something else for the time being. However, this bag, I've noticed something that I didn't on the other 2. I inject a week ago, and lifted it up today to check around the bag and saw on the bottom what you can see in the picture. To be fair, I've never checked the bottoms on the 2 successful growths, so I'm not sure if this is normal. I just wanted to see if anyone had some insights on whether this looks bad, normal, or just ride it out. Any insight would be great! Thank you all.




That is definitely bacterial. I wouldn’t bother keeping it around tbh.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28838913 -

What's the indicator? I don't see it. Is it the yellow spots on the sides,something else?

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Re: Ask Quick Questions, Get Quick Answers [Re: kimura999]
    #28838926 -

kimura999 said:
Hello. First time grower here. Would like a quick check on this grow. I assume this is ready to initiate pinning,right? Has this nice earthy scent :grin:






On another note,i had another batch that got contaminated now a second time in the same container with liner in it.
So the other container had the liner in the container while on this one in the pictures has the liner outside on the box itself. That's the only difference.
The mycelium did not grow into the coco coir for some reason and eventually a different mold grew that releases a weird fermenting scent.
Any idea what might cause such a thing to occur? The spawn was really great(had 4 jars total,used 2-2 for each batch) with abundant growth but afterward it just stalled in the bulk substrate.



We don't initiate pinning. Fae all the time after spawning. Check out the hitchhikers guide in my sig just in case.

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Re: Ask Quick Questions, Get Quick Answers [Re: kimura999] * 1
    #28838948 -

kimura999 said:
What's the indicator? I don't see it. Is it the yellow spots on the sides,something else?



Brother I’m sorry I was a page behind, replying to someone else.

We’d have to see the spawn to tell you 100%, but if it stalls and contams out once you send it to a tub then it wasn’t clean to begin with, unfortunately.

What was your process to make the spawn?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #28838972 -

Thanks! I'll dump it and start working on doing everything from scratch

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Re: Ask Quick Questions, Get Quick Answers [Re: towerofbabel]
    #28838987 -


Having a hard time deciding where to transfer from, any suggestions??


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🅃🄴🄰🄼 🄽🄴🅂🄲🄾
      If you already know everything,
          you can't learn anything.

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Re: Ask Quick Questions, Get Quick Answers [Re: morningWood]
    #28838991 -

If my PF jars are pinning prior to birthing, is there any change in protocol to prepare them for the water tub? Should I still soak them for 24 hours, roll in verm/or coir and put them in the H20 tub?


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Active Species Forage List:
Psilocybe cyanescens
Psilocybe allenii
Gymnopilus luteofolius
Psilocybe pelliculosa
Psilocybe semilanceata
Psilocybe azurescens
Panaeolus cinctulus

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28838994 -

Ahh,no worries. Inoculated popcorn jars in SAB with LC;strain was Hawaii. Waited around 3 weeks for full colonization(did a shake and break in the meantime),every jar had very nice,clean growth,there was no discoloration.
Pasteurized coco coir first then sterilized(2 hour in a pot) it in 4 jars, to make a 1:2 ratio of spawn to bulk substrate ratio. So in this case i used 2 jars(~1liter) of popcorn to 4 jars(~2liters) of coir. Microwaved the coir in the jars afterward the sterilization just to make sure. Waited until everything cooled down.
Mixed them in the SAB. The result was one container getting contaminated while the other is in the pictures doing something at least even if contaminated.
Only difference between the containers was the placement of the liner. The contaminated tub had it inside the good one outside.

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Re: Ask Quick Questions, Get Quick Answers [Re: kimura999]
    #28839001 -

Kdeezy said:
If my PF jars are pinning prior to birthing, is there any change in protocol to prepare them for the water tub? Should I still soak them for 24 hours, roll in verm/or coir and put them in the H20 tub?



Dunk them
kimura999 said:
Ahh,no worries. Inoculated popcorn jars in SAB with LC;strain was Hawaii. Waited around 3 weeks for full colonization(did a shake and break in the meantime),every jar had very nice,clean growth,there was no discoloration.
Pasteurized coco coir first then sterilized(2 hour in a pot) it in 4 jars, to make a 1:2 ratio of spawn to bulk substrate ratio. So in this case i used 2 jars(~1liter) of popcorn to 4 jars(~2liters) of coir. Microwaved the coir in the jars afterward the sterilization just to make sure. Waited until everything cooled down.
Mixed them in the SAB. The result was one container getting contaminated while the other is in the pictures doing something at least even if contaminated.
Only difference between the containers was the placement of the liner. The contaminated tub had it inside the good one outside.



Your spawn was dirty


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Ask Quick Questions, Get Quick Answers [Re: kimura999]
    #28839002 -

Hey friends! I prepped some coir yesterday and it’s a bit over field capacity. Don’t have any verm, may have some gypsum somewhere. Find the gypsum and mix, or get to squishin the water out?

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Re: Ask Quick Questions, Get Quick Answers [Re: NotWimbo]
    #28839006 -

Toss a hand full of gypsum in and get to squishing


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Ask Quick Questions, Get Quick Answers [Re: NotWimbo]
    #28839007 -

Squish squish


--------------------
This hobby has taught me many things.
Most of those things are patience.


The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWars
III

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Re: Ask Quick Questions, Get Quick Answers [Re: Scemilope]
    #28839016 -

Squish squish it is! It was perlite not gypsum :stoned:

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Re: Ask Quick Questions, Get Quick Answers [Re: NotWimbo]
    #28839028 -

Hi! On Monday, I PCed a bunch of no pour agar, but then I tested positive for COVID and have been in self-isolation since. They've been sitting in the unopened pressure cooker, do you think they'll be okay to use as-is once I'm better? Should I redo the PC? Or would I be better off just tossing out all the agar and redoing them completely? Thanks!

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Re: Ask Quick Questions, Get Quick Answers [Re: bijinkei]
    #28839034 -

You're fine, plates should last way longer than you will have covid. Just take care of yourself and when you feel better use the plates. Personally I'd take them out of the PC, I can't think of a real reason to leave them in there if the plates are prepped correctly.

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Re: Ask Quick Questions, Get Quick Answers [Re: kimura999] * 1
    #28839041 -

L
kimura999 said:
Ahh,no worries. Inoculated popcorn jars in SAB with LC;strain was Hawaii. Waited around 3 weeks for full colonization(did a shake and break in the meantime),every jar had very nice,clean growth,there was no discoloration.
Pasteurized coco coir first then sterilized(2 hour in a pot) it in 4 jars, to make a 1:2 ratio of spawn to bulk substrate ratio. So in this case i used 2 jars(~1liter) of popcorn to 4 jars(~2liters) of coir. Microwaved the coir in the jars afterward the sterilization just to make sure. Waited until everything cooled down.
Mixed them in the SAB. The result was one container getting contaminated while the other is in the pictures doing something at least even if contaminated.
Only difference between the containers was the placement of the liner. The contaminated tub had it inside the good one outside.



Did you make the LC yourself? If not, did you test it on agar first?

Pics of the spawn will be very helpful for diagnostics next time if you don’t have them right now. I know before I learned what healthy spawn looked like I thought my extremely bacterial spawn was healthy because it was thick and white.

Also you don’t need to sterilize coir as a sort of general rule. If your coir is really really dirty then yeah, but I’d be looking into a cleaner source if that’s the case. Usually hydrating it is enough.

Mixing the tubs up in a SAB is also totally unnecessary. The spawn is either good or it’s not. All the sterile work in the world won’t make a difference post-inoculation.

But yeah let us know about that LC. That’s probably the culprit


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: bijinkei]
    #28839050 -

bijinkei said:
Hi! On Monday, I PCed a bunch of no pour agar, but then I tested positive for COVID and have been in self-isolation since. They've been sitting in the unopened pressure cooker, do you think they'll be okay to use as-is once I'm better? Should I redo the PC? Or would I be better off just tossing out all the agar and redoing them completely? Thanks!



Take them out of the PC


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28839056 -

the LC was purchased from a reputable source mode edit ( don't know if naming shops directly is allowed or not) as far as i can tell, used heat till the needle was red hot before each inoculation
the LC looked clean,see through,wasn't turbid

Edited by cronicr (07/05/24 02:44 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28839095 -

Can I grow lions mane in the same room I grow cubes? I heard lions mane needs to grow in a cooler area. The room I grow cubes in is around 75-77 degrees.


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Shop: North Spore Boomr Bag   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co Certified Organic All-In-One Grow Bags   Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy   Myyco.com Isolated Cubensis Liquid Culture For Sale


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