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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Cleaning embedded bacteria Mycopatic Tek * 5
    #28829775 -

Hello folks,

I would like to say hello, as its my first post here as an active member of community. I used to browse this space as a spectator, learning and doing my thang.

Recently I faced huge problem with hidden bacteria in one of my cultures (bought online as LC). I managed to get rid of this shit in the way I never seen anyone described here, so I thought it is time to give back some new ideas to experiment with to others.


So, said culture right from syringe was unusable. After five or six transfers it got a wee better, however still nasty, always. Only way I could use this spawn was bag (Fahster style).

So trying make things shorter:

Take few dishes 3-5 and put some shim under them to create an angle. Anything. Folded paper towel, coins. Whatever. Angle. The more steep, the better - there will be more pronounced border between bare plastic and agar making it easier to transfer once myc reaches the agar. I have only some shitty pics, as I had no idea it's gonna be useful method, so I did not document it decently (so it will gonna sucks as a write up, but believe me, it works).
Now pour your agar as close to solidifying moment as you are able to. And be skimpy. Pour just a splash trying to pour it directly where angle is negative, so agar won't "contaminate" major Petri's surface leaving nutrition film on it.
It may sound more complicated than it is, but once you' visualize this steps it's easiest method I've known.



Now place wedge or two 0.5-1 cm from the edge of an agar and wait. My starts to fight for survival pushing all energy into finding food and water.
It works like water agar, but besides nutes you cut off humidity as well, so bacteria ends up with two stiff cocks up her holes.

Below pic of only one plate saved from disposing(summer cleaning) it's PE, also embedded with bacteria, successfully cleaned with this method.
Not very good pic and not very well executed Tek on this one, but worked anyway.
Just to give an idea.



Once myc reaches agar you have two options:
Cut first millimeters of growth to the new plate;
Or: take a swab and swab myc from inbetween - wedge and agar. From bare plastic. It shortens exposition time and it's taken from extremely unfavorable conditions for bacteria. Also swab transfers into new dish very small amount of myc cells.

If I got your attention I may have some time and make proper writeup with documentation and results. Despite it's not scientifically perfect I believe it's worth of sharing.

Love piss noise

Edited by Mycopatic (06/28/24 06:06 PM)

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Re: Cleaning embedded bacteria Mycopatic Tek [Re: Mycopatic] * 1
    #28829784 -

I think you can go ahead and pour it over the whole culture? because the mycelium will come upwards through fast.

Welcome to visible presence btw :cool:

"another cool tek that was developed by Stephen Peele is the hot agar tek. You can simply pour another layer of hot agar on top of the growth layer and the mycelium will crawl through to the top layer leaving the contaminate behind on the first layer. Then simply transfer the new, clean growth to another plate or use for karo or something else."

https://www.shroomery.org/forums/showflat.php/Number/4065034#4065034

Edited by curenado (06/28/24 06:28 PM)

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Re: Cleaning embedded bacteria Mycopatic Tek [Re: Mycopatic] * 1
    #28829791 -

Hi,
Well, thanks, nice to speak with own voice at last. I know hot pour tek. Helped me a lot, however once I had nasty dish and bacteria was following myc very closely, so only way to transfer was to scrap the myc from surface. In the end I saved the culture, but it was hard to perform in the sab manually. Just sharing another method I found easy.
I know also Josex poke biopsy, brf puk, water agar, agar sandwich. Is good to have some tricks to choose depending on what's best ATM :smile:

Cheers :smile:

Edited by Mycopatic (06/28/24 06:48 PM)

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Re: Cleaning embedded bacteria Mycopatic Tek [Re: Mycopatic]
    #28830075 -

simple and smart, great write up!


--------------------
Confused? Well now you can!
HHG - cheapest way to start - how i roll

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Re: Cleaning embedded bacteria Mycopatic Tek [Re: bakedbeings]
    #28830143 -

Thank you.

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Re: Cleaning embedded bacteria Mycopatic Tek [Re: Mycopatic]
    #28830332 -

Mycopatic said:
Hi,
Well, thanks, nice to speak with own voice at last. I know hot pour tek. Helped me a lot, however once I had nasty dish and bacteria was following myc very closely, so only way to transfer was to scrap the myc from surface. In the end I saved the culture, but it was hard to perform in the sab manually. Just sharing another method I found easy.
I know also Josex poke biopsy, brf puk, water agar, agar sandwich. Is good to have some tricks to choose depending on what's best ATM :smile:

Cheers :smile:


OIC.....I saw "never described here before" and missed the part about HA failing to be enough. was thinking more about exposed contam in the dish making it over. Duly noted. in case?


--------------------
Yours in the Natural State Land of Enchantment!

"Sometimes the only way for a man to find true happiness is to take drugs in a group" - Cochise

No makin funna my pomes!

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Re: Cleaning embedded bacteria Mycopatic Tek [Re: curenado]
    #28855231 -

Linking this one for sure. Great stuff there. Simple and working for me right now. Thank you.


--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
πŸ…ƒπŸ„΄πŸ„°πŸ„Ό ​ πŸ„²πŸ„ΎπŸ„½πŸ„³πŸ„ΈπŸ„ΌπŸ„΄πŸ„½πŸ…ƒ ​ πŸ„²πŸ…„πŸ„Ώ

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Re: Cleaning embedded bacteria Mycopatic Tek [Re: DV8DUG]
    #28871835 -

Thank you.
I'm really happy if it helps.
Bacteria can be pita.

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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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