Herme5 said:

H

BeefSupremeJr said:

wtf why does your fp+
culture look better than mine?!

---

He made it with love

---

vicepope said:

Herme5 said:

H

BeefSupremeJr said:

wtf why does your fp+
culture look better than mine?!

---

He made it with love

---

Is that why my looks like hell. No love in my heart.
I do like that 6 out of 7 jars recovered.

---

Whenever I’m told to just throw away something that’s not working, I try to save it, with minimal effort, while advancing other projects. 

That’s why I can’t bring myself to throw away these disgusting, but densely colonized bags.  Keep thinking about casing them, or soaking them and covering with verm and putting them in a water chamber, or burying them in a flower pot, or even spawning to bulk.

At a minimum, I’ll put a sample of each to agar, and eventually I’ll chuck em if they don’t fruit. 



Meanwhile…





Agar pieces stuck all over the jars in inconvenient spots, no matter how much I whack em.  The avery albino agar mostly landed in the grain, but the albino mvp just wanted to be high up out of reach of the grain.  According to a post I found somewhere from a TC, it’s fine, the myc will crawl down to the grain, but laying it on it’s side is also acceptable, so we’ll see how it goes. 

Attempting Pasty’s unmodified lid tek.  Once they’re colonized a bit I’ll give em a good shake, stand upright and loosen the lid for fae.

I have 4 more grain jars to inoculate.  The FP+ plates are just about ready, as are a couple of other plates, but I want to transfer a little wedge to a fresh plate to keep the culture going.  I used my last two blank plates for the two aggressive albinos, but made four new jars of brf agar last night, as well as a jar of water agar.  Haven’t poured anything yet, but I have such plans…. More pics and rambling to follow.




Second flush of Vogon B+ seems to be on its last gasp.  Watching for now.
Why is this APE trich bin still sitting in my grow area?  Who is responsible for this?!

Dkeppel said:

Nats seem super easy, the mycelium eats trich (Too an extent of course, I did lose 2 shoeboxes to trich,  but below plate pin was from a culture that ate trich) and handles bacteria infection well, below also is a picture of 2nd flush of a ziploc that was spawned with bacteria contaminated popcorn.

That is fascinating!  The great Alan Rockefeller recently posted microscopy video of a  Ps. Natalensis x Ps. Cubensis hybrid (external link). Makes me wonder if the trich resistance of Ps. Nat could be bred into Ps. Cub.  Might make all those doomed UBTeks viable .

Same things as a cubes, very closely related to cubes, they enjoy a little more FAE.



Gorgeous.  Strong work!

I'd offer to send you spores and wedges, but I'm from New Zealand and I bet a kind member from America could supply you with better Nats than myself, faster and cheaper

True, but could they supply me with Kiwi Nats?  I doubt it .

I'm really impressed with how many tek's you're going through, you obviously love experimenting.

I find something that works and never change.

That's my problem, haven't found anything that works!  Right now I'm trying to do everything, get a feel for which workflow I wouldn't mind repeating or scaling up, and also building a repertoire of techniques in case I come across a strain that doesn't like whatever main TEK I end up settling on.

For example, I found the 2% Agar and 2% LME recipe for agar 2 years ago, I throw the ingredients into a media bottle, add warm water, shake shit out of it, put bottle into hot water bath on a trivet on the stovetop until melted, shake the shit out of it and put into 10 min vented instant pot on trivet with a loose fitting cap for a 50 min pressure cooking cycle, let it chill for an hour in instant pot once finished to let temp come down, tighten cap, shake shit out of it, let bubbles settle out of agar and then pour, works great and comes out clear

Welp, now I've got another TEK to try.  Thanks!

Edit: If you want to try Nats, but can't find anyone closer to send you something, I'll absolutely hook you up brother! Free of course, but you might be waiting 4 weeks for a letter

I'm in no kind of hurry.  I'll PM you my address in case you're feeling inspired to spread the mush love

---

Dkeppel said:

I was going to try some low tech Nat x Cube Crosses myself, I'll probably try mixing Nat and whatever Cube I fancy spores together on an agar plate, and mixed into sterile water which I'll shot into PF tek cakes, and finally with something like Fahster's method outlined here - https://www.shroomery.org/forums/showflat.php/Number/28117746

aw man, another TEK to add to my growing list

You said no rush, but I was sending mail out to a man in need in NZ anyway. When you've got the SAB setup anyway, you should do everything all at once right?

I always get like this, end up spending hours with the fan off and window closed breathing through a mask and sweating heavily bc I’m also running the PC at the same time and the room turns into a sauna while I’m doing dozens of transfers and any other sterile work I can think of

Edit: Forgot to mention something, you probably alright know this, but I forgot just now and possibly fucked up my agar, if you leave your media bottle lid on too tight while the agar cools before pouring it will create a vacuum like canning jars, note to self don't do that... I only left my lid on a few turns and it did it.

I’ve definitely done the opposite and had them boil over into the pc water.  It’s not an entirely unpleasant smell, but it sure is a pain to clean up…everything’s all sticky

---

ArkaNaut said:

Semi-Solid Liquid Culture


Small amount of added agar thickens and allows better clinging when shaking incoculated grain spawn

---

ArkaNaut said:

Running To-Do List

Clean and Organize Lab Area

• Build grow tent
  
• Gather loose tools and small objects together
  
• Gather and take out trash - Done
  
• Rearrange equipment for better walkthrough flow
  
• Bring lights up and arrange on grow containers
  

Cooking/Pressure Cooking

• Grain + Coir Bottle Teks - Done
  
• PFCakes and PFPucks - Done
  
• Grain Jars - Done
  
• Thicc brf LC
  

Container Prep

• Modify lids with silicon and micropore
  
• Clean used PFJars
  
• Make PF Substrate and Fill Jars - Done
  
• Make various bags of raw rye, water, verm, boozecoir
        * 1c rye, 1c water, 1c verm x 2
        * 1c rye, 1c water, 1c boozecoir x 2
  
• Make bags of just boozecoir to stop fermentation in PC
  

Spawning

• Set up larger water chamber for hydrated PFCakes
  
• Place fully colonized PFCakes into water soak
  
• Shake 6/17/24 jars - they are approximately 30% colonized - Done
  

Sterile Work

• Inoculate Tamp plate into 2 rye jars
  
• Inoculate Dirty Teacher onto PFCakes and PFPucks
  
• Inoculate Distilled water bottles with all available genetics for LI
  
• Inoculate agar jars with clean agar myc
  
• Post images of unusually fast growing myc - is it trich or legit? - if legit plate it on brf agar
  

Documentation

• Write out inventory of all plates, jars, their sources and derivatives
  

I just recieved a pack of LC from my fav vendor.  Mazatapec and Hillbilly, and a free bonus LC of Leucistic Blue Magnolia! 
I'll be branching into edibles, with blue oyster, king oyster, and lion's mane.  I have some supplies for that, but I think I can't reasonably tackle any substantially new TEKs.  Managing the growing Agar library, deciding what transfers to make, managing the PFCakes, Pucks, and the grain jars, is enough. 

My true next step is clearing up my grow area.  Have to move some furniture, set up shelves, tables, decide on storage area, some sort of organizational system, think about investing in a flow hood...

---