underfoot said:

Do the halos indicate something particular is up?

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BTPB said:

I’ve seen some people with some really high number of transfers. I understand doing a couple transfers from the initial plate of spores, but if the second plate is clean and uniform, is there really any advantage of doing additional transfers or is that just to keep the plates alive?

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RichMyco said:

Still learning the search function but I'm having trouble finding any write ups on Master/inoculation jars. Are there any up to date explanations on the process and follow up steps like storage/use? Wondering for educational purposes. Links would be greatly appreciated.


Mushlove!

EDIT: I did see Revs recent post on Li cultures, but I was wondering about alternative options.

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Mr Piggy said:

Fungify said:

Hello, I can't do agar since I don't have a correct setup for the moment.

Still, I have cardboard clones.

1/ Do you think I can expand them on much more cardboard ?
2/ Can I use the result if any, to colonize a more suitable substrate ? (the idea is to reduce the possibility of contamination by increasing the mycelium added at the beginning)

Fungify

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Buddy, we need way more info than that.  Do you mean you have placed stem butts on cardboard from finds of wild mushrooms?  What species are you talking about?  What substrate?  What are your plans here?

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Fungify said:

Sorry, you're right. Here's some explanation :

I have cardboard clones of GT (not wild), the cardboard is colonized.
My thought was, since I did that from stem butts, maybe I can use the colonized cardboard to colonize more cardboard (ratio 1:20 or more), in a layer fashion (approximatively ten sheets).

If that succeed, then I could use them to colonise a pasteurised/sterilised substrate. The substrate would be a mix of straw/dry herbs and composted horse manure (respectively 1/2 1/2, wet for the straw/herbs).
To maximise the colonisation, I would proceed in layers: substrare - cardboard - ... - substrate - cardboard.


Is this project a complete naive bullshit, or was it done before ? (I couldn't find any corresponding info on the seach engine :/)

Hope I correcly answer you.

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underfoot said:

underfoot said:

Hey again folks, I would appreciate if some better trained eyes could have a look at this transfer I made a few days ago. And FYI, the growth was perfectly uniform before yesterday when I made two further transfers from the lower part of this plate.

The thing is, my agar plates were poured hot, which I think led to a layer of water on top of the agar, and the mycelium is very wet as a result. But, I'm not sure what I can do about it now.

My question is: does this water-drenched growth look like cube myc to you or am I simply growing mold? Hopefully my pictures provide enough detail. Thanks!



Both pictures are of the same plate.

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The three transfers I've taken from the plate above look like this now:

Plate #1:


Plate #2:


Plate #3 (this transfer was made just 2 days ago with a new recipe):


How do these plates strike you? Sendable once grown? To be honest they look similar to what I transferred from, but the growth looks uniform and quite nice to my eyes despite being very tomentose.

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This is what the edge of the halo looks like up close. Those little nubs are emerging rhizos. I have yet to observe a "halo" like that form from any foreign fungi, and I have looked at a lot of them up close. Maybe too many hours of my life even, staring at leading edges.



IME those halo styles are a genetic response to conditions. Your agar is the most likely cause for weird growth.

Nichrome said:

underfoot said:

underfoot said:

Hey again folks, I would appreciate if some better trained eyes could have a look at this transfer I made a few days ago. And FYI, the growth was perfectly uniform before yesterday when I made two further transfers from the lower part of this plate.

The thing is, my agar plates were poured hot, which I think led to a layer of water on top of the agar, and the mycelium is very wet as a result. But, I'm not sure what I can do about it now.

My question is: does this water-drenched growth look like cube myc to you or am I simply growing mold? Hopefully my pictures provide enough detail. Thanks!



Both pictures are of the same plate.

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The three transfers I've taken from the plate above look like this now:

Plate #1:


Plate #2:


Plate #3 (this transfer was made just 2 days ago with a new recipe):


How do these plates strike you? Sendable once grown? To be honest they look similar to what I transferred from, but the growth looks uniform and quite nice to my eyes despite being very tomentose.

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This is what the edge of the halo looks like up close. Those little nubs are emerging rhizos. I have yet to observe a "halo" like that form from any foreign fungi, and I have looked at a lot of them up close. Maybe too many hours of my life even, staring at leading edges.



IME those halo styles are a genetic response to conditions. Your agar is the most likely cause for weird growth.

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@Nichrome, what factors would influence weird growth, in agar?

Carmelization? Ph?


@Underfoot
I bought a lighted jewelers loupe, it definitely helps to identify potential contams more promptly, and germination also.

Similar to this
https://www.amazon.com/JARLINK-Illuminated-Jewelers-Magnifier-Foldable/dp/B078N34WZR/ref=asc_df_B078N34WZR?tag=bngsmtphsnus-20&linkCode=df0&hvadid=80676783691785&hvnetw=s&hvqmt=e&hvbmt=be&hvdev=m&hvlocint=&hvlocphy=&hvtargid=pla-4584276309244573&psc=1

alligator said:

Quick question though for people with a Presto. Does your little valve that pops up to indicate pressure (says "top" on it) come up while it's venting? The instructions act like it's not supposed to pop up until I put the weight on but it usually happens about 1 minute into the venting for me.

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LewDoja said:

I hadn't realized that it was fractions of a percent, difference.  This can be  game changing. And I will be more precise in my weights and measurements.
Thanks

Side bar where is that picture in your sig? FUCKING BEAUTIFUL

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Trippyskies420 said:

Hey so I made a pasty tub but am worried about fruit Flys and fungus gnats. I was thinking if I added more holes and added 1 layer of micropore tape over the holes that might be a preventative measure.

Am I stupid? If not do you think 2 extra holes on the long sides and 1 hole on each short side would be enough FAE? I do live in a humid area as well. Thanks!

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