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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: ansr]
    #28799394 -

What do you guys do with any plate pins that you don’t end up cloning?


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For it is only Darkness that crafts one to thrive in the Light. As illumination of the soul, its brilliance unmatched.
               
                    πŸŒ•πŸŒ–πŸŒ—πŸŒ˜πŸŒ‘πŸŒ’πŸŒ“πŸŒ”πŸŒ•
  :sporedrop:Gymnopilus subspectibilis grow log:sporedrop:             
β€’89g fresh subspectibilis teaβ€’
β€’RapΓ©h Craftersβ€’
β€’ Gift yourself a healthy dose of Self banningβ€’

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Re: Ask Quick Questions, Get Quick Answers [Re: Soloist] * 3
    #28799395 -

Nothing, usually.


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Soloist] * 1
    #28799398 -

They go in the pit with everything else that doesn't make the grade.


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Question everything, it's very responsive.
culture storage

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28799400 -

What do you guys think is in this plate? It's not growing on top or on bottom, it's looks to be more within the agar. I hadn't changed my process which has severed me well for years. The only thing I can think of is maybe the LME is too old? The agar recipe (LME and agar) as well as the PC process used were the same as I've always used but all of a sudden this started showing up in most the plates. Do you think it's some sort of sediment and the plate is okay to use?



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Re: Ask Quick Questions, Get Quick Answers [Re: Threads2theSoul] * 1
    #28799422 -

Honestly. That kind of looks like the remnants of potato flake when I don’t completely dissolve it in my agar. Just watch it.


--------------------

The Shroomery Manual
Wanna Play?
Dream Interpretation
GC’s Ayahuasca Recipe
Free Therapy

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #28799450 -

Yeah, solids.


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Question everything, it's very responsive.
culture storage

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Re: Ask Quick Questions, Get Quick Answers [Re: Soloist] * 5
    #28799452 -

Soloist said:
What do you guys do with any plate pins that you don’t end up cloning?


Majority I’ll say a pray and flush them, but if they are pretty or chubby, I’ll play taxidermist and keep them in cryovials for an afterlife.



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β€œAll this buttoning and unbuttoning”

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Re: Ask Quick Questions, Get Quick Answers [Re: 999999999] * 1
    #28799472 -

Gleyck said:
Soloist said:
What do you guys do with any plate pins that you don’t end up cloning?


Majority I’ll say a pray and flush them, but if they are pretty or chubby, I’ll play taxidermist and keep them in cryovials for an afterlife.




Haha, that’s cool.

I hate to waste and, hate disposing of life so..I’ve been freezing them little by little to add to tea down the line :shrug:


--------------------
For it is only Darkness that crafts one to thrive in the Light. As illumination of the soul, its brilliance unmatched.
               
                    πŸŒ•πŸŒ–πŸŒ—πŸŒ˜πŸŒ‘πŸŒ’πŸŒ“πŸŒ”πŸŒ•
  :sporedrop:Gymnopilus subspectibilis grow log:sporedrop:             
β€’89g fresh subspectibilis teaβ€’
β€’RapΓ©h Craftersβ€’
β€’ Gift yourself a healthy dose of Self banningβ€’

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
    #28799480 -

DERRAYLD said:
You'll have to replace the pre filter and hepa regularly if you leave it on 24/7.


That and my room is small, I had to get fancy to fit a 3x2. It gets warm. If left on it would be like a sauna up in there. Even after an hour...


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πŸ…ƒπŸ„΄πŸ„°πŸ„Ό πŸ„½πŸ„΄πŸ…‚πŸ„²πŸ„Ύ
      If you already know everything,
          you can't learn anything.

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Re: Ask Quick Questions, Get Quick Answers [Re: Soloist] * 1
    #28799481 -

Patience you must have, my young padawan

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Re: Ask Quick Questions, Get Quick Answers [Re: Soloist]
    #28799502 -

Soloist said:
What do you guys do with any plate pins that you don’t end up cloning?


I just use em as extra inoculation points.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: ansr]
    #28799540 -

ansr said:
The first print I made failed I think. It was very light and had water drops on the print but I let it dry and tried to get it into a syringe but that was easier said than done. It seems like the spores got stuck to the plastic on the inside of the syringe. :thumbdown:
I made some jars just to try and see if it works anyway.


This is my second print, looks much better. Now I need to learn how to properly get it into a syringe.



You make as many spore syringes as you want. I do.

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Re: Ask Quick Questions, Get Quick Answers [Re: The Tao] * 2
    #28799582 -

As others have mentioned a print is better than a MSS. Making a MSS just dilutes the spores and creates additional unnecessary vectors.

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Re: Ask Quick Questions, Get Quick Answers [Re: CocaineBuffet] * 1
    #28799739 -

The Tao said:
ansr said:
The first print I made failed I think. It was very light and had water drops on the print but I let it dry and tried to get it into a syringe but that was easier said than done. It seems like the spores got stuck to the plastic on the inside of the syringe. :thumbdown:
I made some jars just to try and see if it works anyway.


This is my second print, looks much better. Now I need to learn how to properly get it into a syringe.



You make as many spore syringes as you want. I do.



:grin:

CocaineBuffet said:
As others have mentioned a print is better than a MSS. Making a MSS just dilutes the spores and creates additional unnecessary vectors.


I'm doing PF tek though.

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Re: Ask Quick Questions, Get Quick Answers [Re: ansr]
    #28799911 -

Still learning the search function but I'm having trouble finding any write ups on Master/inoculation jars. Are there any up to date explanations on the process and follow up steps like storage/use? Wondering for educational purposes. Links would be greatly appreciated. :crazy2:


Mushlove! :mushroom2:

EDIT: I did see Revs recent post on Li cultures, but I was wondering about alternative options.


--------------------


The Hitchhikers Guide (DON'T PANIC)

Edited by RichMyco (06/04/24 07:03 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: RichMyco]
    #28800014 -

When do you know when to take a second transfer when cleaning on agar? I made my first transfer when it looked like this:


It's been about 5 days and they have definitely spread out on the dishes but the myc is so light that I couldn't even good a picture of it. It's probably spread out about an inch from the center. But it looks like it's still very delicate at this point.

Then I took another couple transfers from the original dish 2 days ago because the myc had gotten much thicker and ropier, so it looked stronger. That dish has already gotten some extra  sprouts off it and they are much thicker strands.

Besides my wonky, not thoroughly mixed plates, I don't see much in the way of contamination. Should I just let them go until they are more solid white and ropey? Or is there a tek I'm missing that shows when it's good to make the next transfer?


--------------------
If you're new, read the Hitckhiker's Guide first. Notice the PF Tek advice in Chapter 1 for a great starting place. :thumbup:

Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: alligator]
    #28800023 -

This is about as good as I could get. You can see I put 3 transfers on this same dish. It's spreading out but it's very light looking. Should I just leave it a while longer? Like I said, this is about 5 days of growth since that picture above.


Here is the original plate today, obviously a little mangled from transfers being taken:


And here is the transfer I took 2 days ago after it got a lot fuller. Not as much growth but it's coming in a lot thicker.


--------------------
If you're new, read the Hitckhiker's Guide first. Notice the PF Tek advice in Chapter 1 for a great starting place. :thumbup:

Hitchhiker's Guide to the Shroomery

Edited by alligator (06/04/24 09:42 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: underfoot]
    #28800048 -

underfoot said:
Hey again folks, I would appreciate if some better trained eyes could have a look at this transfer I made a few days ago. And FYI, the growth was perfectly uniform before yesterday when I made two further transfers from the lower part of this plate.

The thing is, my agar plates were poured hot, which I think led to a layer of water on top of the agar, and the mycelium is very wet as a result. But, I'm not sure what I can do about it now.

My question is: does this water-drenched growth look like cube myc to you or am I simply growing mold? Hopefully my pictures provide enough detail. Thanks!



Both pictures are of the same plate.


The three transfers I've taken from the plate above look like this now:

Plate #1:


Plate #2:


Plate #3 (this transfer was made just 2 days ago with a new recipe):


How do these plates strike you? Sendable once grown? To be honest they look similar to what I transferred from, but the growth looks uniform and quite nice to my eyes despite being very tomentose.

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Re: Ask Quick Questions, Get Quick Answers [Re: underfoot] * 4
    #28800050 -

I'm never a fan of the halo around mycelium like that, your 3rd plate has such a large halo it would concern me.

I'd let that plate continue to grow to see if the growth becomes more organized.

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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
    #28800060 -

Do the halos indicate something particular is up?

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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