Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery


This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com APE Liquid Culture For Sale   Mushroom-Hut Mono Tub Substrate   North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Autoflowering Cannabis Seeds

Jump to first unread post Pages: < First | | 1251 | 1252 | 1253 | 1254 | 1255 | 1256 | 1257 | 1258 | 1259 | 1260 | 1261 | 1262 | 1263 | 1264 | 1265 | 1266 | 1267 | 1268 | 1269 | 1270 | 1271 | | Last >
Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator] * 1
    #28650761 -

AspectOfTheCreator said:
My t1 plates always grow out clean. Is it risky or bad practice to use these for inoculating grain rather than going to t2 first?


Yes and no. I’m gonna give my opinion but it may be contentious.

Last few months I’ve sent a lot of multispore T1s to bulk and pretty much all of them have given me weird and undesirable growth. T2 - T3 has been a lot more “together.” I think at least T2 is the smart option because it weeds out the less stable genes, the stuff you wouldn’t select for a transfer anyway. Taking it out a couple of transfers isn’t always for insuring cleanliness, but to also make sure your genes are a little more compatible with each other.

I haven’t read this, I haven’t confirmed this, this has just been my observation.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator] * 1
    #28650947 -

AspectOfTheCreator said:
My t1 plates always grow out clean. Is it risky or bad practice to use these for inoculating grain rather than going to t2 first?


I wouldn't trust a T1 if I didn't have a T2 to prove that at least the sector I took from was growing clean.

Making clean grain spawn is my least favorite and most laborious part of this hobby so I would rather lose a plate than a jar/bag.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: CocaineBuffet]
    #28651215 -

both agreed, at the same time i do think that it really depends,
t1 CAN be perfectly fine and clean.
not to give out a recommandation by any means, but well i have inoculated grain with wedges from a germination plate and it was fine, but i would agree 100%, t2 is the safe route and allows for a somewhat more stable culture.


--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: chris77] * 2
    #28652095 -



Some really beautiful cultures. TTBVI clone looking really good.


--------------------
Astra said:

“Peace River has a gentle personality, yet TTBVI hit me like strong LSD with a twist of medical grade meth. Not for the faint of heart.”

Team Panaeolus

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: pablotx] * 1
    #28652098 -

pablotx said:
APE from a (purchased) MSS that had germinated... to basically an LC to agar. This is the first transfer.




I would take the slice from 5 to 6 o’clock. That’s the best part of that in my opinion.


--------------------

The Shroomery Manual
Wanna Play?
Dream Interpretation
GC’s Ayahuasca Recipe
Free Therapy

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: GenesisCorrupted]
    #28652130 -

I've noticed this in the past, but never really thought much about it. Some cultures appear to "blue" when they run out of room to colonize. Is this a known characteristic of cubensis?

This sample has room to grow, but is competing with it's brother in the middle of the agar dish.



--------------------
LAGM 2.024



The Hitchhiker's Guide to the Shroomery | Pastywhyte’s Ez dialed monotubs | Clean spawn checklist for the new grower | How I do Rye Berry Prep | Frank's Simple Coir/Verm Tek | Stro's Cleaning and Isolating On Agar

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: mycosispeon] * 3
    #28652180 -


A crappy picture of my Bluey Vuitton culture. I have to work out of a SAB rn so I couldn't get it to focus at all well.

:teabird:


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room

IRC: ##mycology on Libera.chat come hang!

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: mushhead] * 2
    #28652309 -

I think I like this one.  PES Amazonian, clone.





--------------------
Sadly remembering when the Shroomery wasn't a closed minded mutual admiration society for OCD cargo cultists.  RIP methodical science.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: mycosispeon]
    #28652349 -

mycosispeon said:
I've noticed this in the past, but never really thought much about it. Some cultures appear to "blue" when they run out of room to colonize. Is this a known characteristic of cubensis?



When I have encountered that in the past, it was a penicillium contamination.


--------------------
Sadly remembering when the Shroomery wasn't a closed minded mutual admiration society for OCD cargo cultists.  RIP methodical science.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: OldManRiver]
    #28652352 -

Could you please post a picture to show us what an example of that is?

I like lurking here. :3


--------------------

The Shroomery Manual
Wanna Play?
Dream Interpretation
GC’s Ayahuasca Recipe
Free Therapy

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator] * 1
    #28652364 -

AspectOfTheCreator said:
My t1 plates grow out clean. Is it risky or bad practice to use these for inoculating grain rather than going to t2 first?


I'll use them without concern.  I know that my transfer process is clean.  If you are confident that your transfers are reliable, I wouldn't hesitate. 

If the original plate was gross, I'd do a T2.


--------------------
Sadly remembering when the Shroomery wasn't a closed minded mutual admiration society for OCD cargo cultists.  RIP methodical science.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: GenesisCorrupted]
    #28652374 -

GenesisCorrupted said:
Could you please post a picture to show us what an example of that is?

I like lurking here. :3


I can't find the pic.  It was a well organized culture on the plate, and the center turned blue grey.  The edges looked like good myc.


--------------------
Sadly remembering when the Shroomery wasn't a closed minded mutual admiration society for OCD cargo cultists.  RIP methodical science.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: chris77]
    #28652425 -

mycosispeon said:
I've noticed this in the past, but never really thought much about it. Some cultures appear to "blue" when they run out of room to colonize. Is this a known characteristic of cubensis?


I'm not too sure if it's a characteristic of cubes, but I would like to know as well. Chiming in because I have a culture of Storm Trooper on an LMEA plate that is doing this. The rhizomorphic growth towards the edge of the plate has a lot of bluing on some of its ropes. It even pinned a bit, & the tips of some of the pins got really dark blueish. Will post a photo in the morning for reference


--------------------
"I'm a new Christ Savior - Neo from the Matrix
Young God negu, can't fuh with no lame shih
High off psychs, yeah, a negu stay t.a.d.e.d - Philanthropist - The Underachievers

T.A.D.E.D - Trippy All Day Every Day

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: MasterNeo] * 1
    #28652426 -

are you preparing your agar on the dry side?


--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: chris77]
    #28652432 -

I think that plate was a more wet agar mix. Possibly I did 7g agar with 10g LME
I've been doing most of my plates recently in that ratio, or 7g agar to 7g LME for more wet, & lower nutrient plates


--------------------
"I'm a new Christ Savior - Neo from the Matrix
Young God negu, can't fuh with no lame shih
High off psychs, yeah, a negu stay t.a.d.e.d - Philanthropist - The Underachievers

T.A.D.E.D - Trippy All Day Every Day

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: GenesisCorrupted]
    #28652592 -

GenesisCorrupted said:
pablotx said:
APE from a (purchased) MSS that had germinated... to basically an LC to agar. This is the first transfer.




I would take the slice from 5 to 6 o’clock. That’s the best part of that in my opinion.


Thanks... will do!

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: chris77] * 1
    #28652634 -



Not sure if you can see the bluing very well with the lighting, but this was what I was referring to


--------------------
"I'm a new Christ Savior - Neo from the Matrix
Young God negu, can't fuh with no lame shih
High off psychs, yeah, a negu stay t.a.d.e.d - Philanthropist - The Underachievers

T.A.D.E.D - Trippy All Day Every Day

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: MasterNeo]
    #28652656 -

Hello! I have some fluffy (but I think clean) mycelium on T2 plates. I followed HazyHorse’s no pour tek and used D3s recipe. I wonder if, given my tomentose growth, I made the plates too nutrient dense—though I weighed all my  agar ingredients with a scale. Anyhow, I was wondering what you all thought about these plates. :tongue2: If they keep growing out nicely, would you transfer again or go to grain? Thank you!!

T2 a



T2 b


T2 c


--------------------
spinning, spinning, spinning... spun
💃🏻💀⚡️

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: chris77]
    #28652767 -

chris77 said:
are you preparing your agar on the dry side?


pretty standard recipe 10g agar, 8g ME, 500ml water. would that be considered "dry"?


--------------------
LAGM 2.024



The Hitchhiker's Guide to the Shroomery | Pastywhyte’s Ez dialed monotubs | Clean spawn checklist for the new grower | How I do Rye Berry Prep | Frank's Simple Coir/Verm Tek | Stro's Cleaning and Isolating On Agar

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: mycosispeon]
    #28652988 -

mycosispeon said:
chris77 said:
are you preparing your agar on the dry side?


pretty standard recipe 10g agar, 8g ME, 500ml water. would that be considered "dry"?


no, not really


--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

Extras: Filter Print Post Top
Jump to top Pages: < First | | 1251 | 1252 | 1253 | 1254 | 1255 | 1256 | 1257 | 1258 | 1259 | 1260 | 1261 | 1262 | 1263 | 1264 | 1265 | 1266 | 1267 | 1268 | 1269 | 1270 | 1271 | | Last >

Shop: Myyco.com APE Liquid Culture For Sale   Mushroom-Hut Mono Tub Substrate   North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Autoflowering Cannabis Seeds

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* oops drunk moment... beer + agar dont splice.. buggers 986 2 09/21/05 01:02 AM
by buggers
* agar question madsick 657 6 04/05/08 12:23 AM
by madsick
* agar
( 1 2 all )		 mikearch 2,579 21 10/05/02 03:52 PM
by mikearch
* Where to find agar around town? HUBSonDUBS 1,042 8 12/24/06 12:12 AM
by Workman
* Mycelium or Mold? on agar beknowdo 14,181 6 01/29/07 02:07 PM
by RogerRabbit
* ? re: LB-agar, also seeking the mycologist aka Erliquin Lefty 3,068 3 05/24/04 03:09 PM
by Lefty
* Agar Work Myc0Maniac 2,908 14 05/13/05 10:17 AM
by Roadkill
* Horse_Meister - Agar Attempts - Log with PHOTS - Open to Experienced Advice Horse_Meister 2,696 14 01/12/08 10:08 PM
by Blutjager

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
783,752 topic views. 15 members, 677 guests and 202 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.049 seconds spending 0.023 seconds on 16 queries.