GenesisCorrupted said:

So I can just poke the loop in there bring it in red hot and just spray it with alcohol instantly.

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DRAKENCL said:

Do they grow stagnant or do they simply rot? Any opinion?

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SirPsycho said:

Mr Piggy said:

I literally bend a lil piece of bailing wire into a loop and use that

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Proper nichrome loops are so good though. It's like a scalpel vs an x-acto. Yeah, they are pretty much the same thing and yeah they both get the job done but the scalpel and the nichrome loop are just so much nicer to use.

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GenesisCorrupted said:

Welp. Looks like you gotta burn your house down.
jk

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Pseudomonas tolaasii is the usual culprit with blotch on mushrooms.

This Pseudomonas fluorescens I isolated came off a wild Cordyceps militaris I found last summer. The cordy sat in a sealed test tube for close to two weeks before I tried to plate up a piece. All that grew was several bacteria. The cordy looked pristine still. No rot of any kind. No slime. I think the bacteria is more interesting than the cordyceps. At first I was bummed but then turned lemons into lemonade.



There are a lot of different Pseudomonas. They are very common.

Smellyhobbit said:

So I’ve got like triple the amount of tubs in my grow space as usual and the substrate wont stop sweating.

Is this just going to be as issue with that many tubs? Any way to mitigate all the water pooling up around the edges? Having warm pools of water has been causing a lot of bacterial jelly to form, and in some cases it’s been bad enough to stunt growth.

Should I just not stack tubs? Grow less?

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skrontz said:

how can i more successfully harvest this second wave after pulling a lot of mushrooms?

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You can get these. It makes cutting down to the sub so much easier while working in a tub.

Bigworm said:

skrontz said:

how can i more successfully harvest this second wave after pulling a lot of mushrooms?

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You can get these. It makes cutting down to the sub so much easier while working in a tub.

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mycosispeon said:

QQ in relation to this post...

I did a second "break and shake" on these Mazatapec inoculated rye berries 5 days ago when they were between 75-90% colonized.

This was just before I did it on 1/31


This is today


They look clean to me, no signs of adversity (moisture, metabolites, etc), but are they fully colonized and ready to spawn?

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arnold582 said:

2 weeks ago, I started another jar using a MP filter, but this time around I changed it after PC'ing. I thought this might solve the gas exchange issue (which I thought stemmed from gummed up tape pores), but just like my previous batch, this one stalled out after shaking without any visible myc growth after ~4 days. I was surprised because I had really strong mycelium growth before (sadly no pics).

At first, I thought the jar must've been contaminated, even though there were no signs of it other than lack of myc growth, but then I carefully loosened the lid inside my SAB and 24h later, I have visible mycelium growth again.

Would it be fair to conclude that this is almost certainly a gas exchange issue?

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I would say so, yes. I've had the exact same situation happen to me with too little gas exchange. Popping the lid produced myc the next day. So I concluded same as you

That being said, changing your filter, whether it's MP, polyfill, paper sticker, is not a good idea. It's supposed to be sterilised with the jar and not tampered with afterward. I did replace paper filters with sterilised polyfill, in my sab, but it's definitely not recommended.

Also, 4 days is a little early to draw conclusions. I've had jars take 1-2 week before showing any sign of colonisation.

arnold582 said:

I'm not sure if my gas exchange hole is too small (3mm or 1/8"), or if this MP tape is just ass. It's not 3M branded, mind you, but it's supposed to be nearly identical, it's described as surgical, paper-based, breathable tape.

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I'm not sure about the MP you use, but when I switched from polyfill to paper filters, keeping one 10mm hole was way insufficient. Super slow colonisation, popping the lids needed.

Here I'm testing my proven polyfill setup against 2x10mm paper filter holes, on 2 strains.

I don't know what size your jars are, but 3mm could sound a wee small



Good luck with your new lid!

DERRAYLD said:

Just FYI,  mp tape has to be 4 layers minimum or it's just letting contam in due to the large pore size of mp tape.

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