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Re: Ask Quick Questions, Get Quick Answers [Re: Camera93]
    #28466350 -

Camera93 said:
MicroMycelium said:
Anyone ever attempt to sterilize and reuse their substrate from a spent tub?

Like..
Throw it in a bag, break it up, throw the bag in the PC, and rehydrate to field capacity after the PC cycle?

Obviously the heat would kill any left over mycelium, but the coir and left over grains would be essentially re-sterilized and could be colonized again.
Feels like an interesting experiment to reduce waste



endless sub tek

mind at large has a write up



Dope!
Thanks

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Re: Ask Quick Questions, Get Quick Answers [Re: WyoMX] * 1
    #28466411 -

WyoMX said:
DERRAYLD said:
And?
Did you suddenly notice an increase in contamination?
Doubt it.

Experience tells me it's not an issue but everyone can do whatever they want.
I choose not to be afraid of my shadow.



There was a thread some years ago I saw that someone here purposely left I think it was 2 super trich'd out tubs open in his grow room next to his other tubs and all the other tubs he had were completely fine and went 2 flushes or more. I'm not saying it's best practice to be leaving moldy tubs everywhere buy I do agree that some people are way to worried about it. I don't worry at all about letting a tub with trich finish maturing



Got to be this thread. SmartAttack is a legend.


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc]
    #28466472 -

Has anyone compared their sterile work after running an air purifier compared to without?

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Re: Ask Quick Questions, Get Quick Answers [Re: MicroMycelium]
    #28466506 -


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Re: Ask Quick Questions, Get Quick Answers [Re: Eyesee3]
    #28466530 -

Eyesee3 said:
Blue king oyster, is this just fae
https://files.shroomery.org/files/23-37/454745232-IMG_20230912_120753794.jpg



a big lack of it it appears


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can


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Re: Ask Quick Questions, Get Quick Answers [Re: Camera93]
    #28466701 -

First time using red dye for my agar and I feel like it's bad luck. I always went blue prior.

What would cause such sporadic and wild myc growth? Could it be I screwed up the batch of agar? I haven't done that in a long time so Im a little confused. Not all myc plates are like this and these are the worst offenders, but it's far more common with this last batch of agar. Even the good looking plates seem a bit off but maybe it's just the red placebo effect. :lol:



edit: coming as transfers from clones I totally expect the myc to be clean by now.


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Edited by ShroomNugget (09/12/23 06:48 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: ShroomNugget]
    #28466715 -

Red dye is toxic to mushrooms. Utsf and I'm sure you'll find out why.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: ShroomNugget]
    #28466716 -

:whathesaid:

Erythrosine (aka Red Dye #3) supposedly has antifungal properties.

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Re: Ask Quick Questions, Get Quick Answers [Re: HILLBILLY OUTLAW]
    #28466717 -

Smellyhobbit said:
Why do you think gloves make things more sterile?



I'm just sticking to what I know. I only started this year, about 7 months ago, and this is the first I've read people neglecting to use gloves....but skin flakes off and hairs fall out, no? Not a big enough problem for you guys?

KeyMaker said:
ShroomNugget said:
tryptkaloids said:
I'm trying to tell you that's all unnecessary. Wash your hands and forearms.

I use my sab naked 90% of the time so sweat ain't an issue.



You can have fun with that. I rather not risk 4 lbs grow bags and all that effort for a higher chance of failure. No gloves is risky AF IMO. No sleeves? That's fair, but I prefer to stay on the safe side.



There are plenty of very successful growers on here who don't use gloves.




HILLBILLY OUTLAW said:
I don’t use gloves. Or isopropyl alcohol. :justdontknow:

I used to but have since phased them out.



So you don't wipe anything down before throwing it into the SAB?

Im open to change considering the number of people here saying otherwise. But you know when I first started out 7 months ago, all the tutorials were all like "ZOMG GLOVES, MASK, TYVEK SLEEVES, WIPE EVERY SINGLE THING DOWN IN ISO BEFORE PUT IN SAB CUZ ZOOOOMG CONTAMS EVERYWHERE!"


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Re: Ask Quick Questions, Get Quick Answers [Re: PBJ710]
    #28466721 -

tryptkaloids said:
Red dye is toxic to mushrooms. Utsf and I'm sure you'll find out why.



PBJ710 said:
:whathesaid:

Erythrosine (aka Red Dye #3) supposedly has antifungal properties.



Well seriously FML I have 25 red plates waiting to be used and 15 plates growing out. Ef Ehm Ehl to kingdom come, that's annoying.

Thanks for the info guys. I assume it's still safe to transfer red onto another plate, let it grow out, and transfer again to a clean plate to save it?

God damn this sucks and I'm very unhappy lol.


--------------------

Edited by ShroomNugget (09/12/23 07:06 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: ShroomNugget]
    #28466722 -

I'm sure you can save it.

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Re: Ask Quick Questions, Get Quick Answers [Re: Kinoko314]
    #28466741 -

Could I get a check on my first two shoeboxes? I'm wondering if it's contam but hoping that it's just dehydration. The fuzzy feet on the right side of the second image concerns me. Should I wait more or harvest what's already there?




Edit: These are APEs, grown with UB. I know, a little ambitious, and easy for contam to happen but hoping it pans out.

Edited by chaotozen (09/12/23 07:25 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: PBJ710]
    #28466742 -

tryptkaloids said:
Red dye is toxic to mushrooms. Utsf and I'm sure you'll find out why.



PBJ710 said:
:whathesaid:

Erythrosine (aka Red Dye #3) supposedly has antifungal properties.




Mine have been growing fine on red dye for the last two weeks with zero issues :ifyoucanawe:

Edit-
Mine is red dye 40 aka “Allura Red” not #3 erythrosine…

I can’t believe fungus is that picky

Edited by MicroMycelium (09/12/23 07:28 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: ShroomNugget] * 4
    #28466754 -

ShroomNugget said:
Smellyhobbit said:
Why do you think gloves make things more sterile?



I'm just sticking to what I know. I only started this year, about 7 months ago, and this is the first I've read people neglecting to use gloves....but skin flakes off and hairs fall out, no? Not a big enough problem for you guys?

KeyMaker said:
ShroomNugget said:
tryptkaloids said:
I'm trying to tell you that's all unnecessary. Wash your hands and forearms.

I use my sab naked 90% of the time so sweat ain't an issue.



You can have fun with that. I rather not risk 4 lbs grow bags and all that effort for a higher chance of failure. No gloves is risky AF IMO. No sleeves? That's fair, but I prefer to stay on the safe side.



There are plenty of very successful growers on here who don't use gloves.




HILLBILLY OUTLAW said:
I don’t use gloves. Or isopropyl alcohol. :justdontknow:

I used to but have since phased them out.



So you don't wipe anything down before throwing it into the SAB?

Im open to change considering the number of people here saying otherwise. But you know when I first started out 7 months ago, all the tutorials were all like "ZOMG GLOVES, MASK, TYVEK SLEEVES, WIPE EVERY SINGLE THING DOWN IN ISO BEFORE PUT IN SAB CUZ ZOOOOMG CONTAMS EVERYWHERE!"



Basically think of it like this. Proper sterile procedure with gloves is the same as without gloves. Your hand shouldn’t be over an open dish or jar regardless. And gloves aren’t sterile anyway. Once you open a box of gloves it has shit from the air all up in it. Once you put the gloves on your hands they have stuff from the open air on them.

And isopropyl why? It doesn’t sterilize, so what’s the point? SABs work because the air inside is still, not because the walls of the box are clean.

Do what makes you comfortable, but if you do things wrong even with gloves you’ll have problems. If you’re not having problems, it isn’t because you’re wearing gloves.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: ShroomNugget] * 2
    #28466761 -

HILLBILLY OUTLAW said:
I don’t use gloves. Or isopropyl alcohol. :justdontknow:

I used to but have since phased them out.



So you don't wipe anything down before throwing it into the SAB?

Im open to change considering the number of people here saying otherwise. But you know when I first started out 7 months ago, all the tutorials were all like "ZOMG GLOVES, MASK, TYVEK SLEEVES, WIPE EVERY SINGLE THING DOWN IN ISO BEFORE PUT IN SAB CUZ ZOOOOMG CONTAMS EVERYWHERE!"



I do not. My jars get shaken and loosened outside the box before they go inside. I pre make my grain jars ahead of time and sometimes they’ll set for a month on the shelf before I knock them up.  I don’t wipe them down prior to use. I don’t wipe down my agar plates before session either. I’m not saying this is good practice by any means but it’s my rhythm and it works for me.
I also don’t keep all my eggs in one basket so to speak so if a jar here or there contams out its a non issue.


--------------------

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 3
    #28466772 -

I dont use gloves, or wipe anything down anymore. I don't even spray the SAB. I haven't noticed any difference between gloves or not, or using iso and not, so I just go for it. I let it settle for like 5 mins and I use a little hand sanitizer before I get started and that's about it. Only contam I had was on 1 cake, I got 2 flushes out of em so I just tossed em when I saw the trich.


--------------------
How would you feel if you hadn't eaten breakfast?

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Re: Ask Quick Questions, Get Quick Answers [Re: MicroMycelium]
    #28466800 -

I went straight to the label on mine and it has both #40 and #3. :frown:


--------------------

Edited by ShroomNugget (09/12/23 08:02 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28466825 -

Harvested my shoebox of golden teachers. Ended up with a pretty good pinset. Harvested a day late. But it seems like alot of the fruits were pretty skinny. I assume this mostly has to do with genetics? Also them being skinny they don't take as long in the dehydrator. I did 140 F for 10-12 hours. Seems like they're "burnt"? How do they look to yall.


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Re: Ask Quick Questions, Get Quick Answers [Re: Chumbum] * 3
    #28466845 -

They’re not burnt, that’s just spores.

Personally I don’t recommend dehydrating for less than 24 hours, especially if you’re going for a lower temp. I know things CAN dry in smaller batches faster, but 10-12 hours is sketchy. I’d put them back on the dehydrator over night.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 2
    #28466859 -

I gotcha. I guess me being new to growing. I'm kinda sketched out by thinking I'm going to overcook them or burn them. But I think I read somewhere it takes much higher heat to burn off the psilocybin or psilocyn? Correct?

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