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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns] * 1
    #28464115 -

RoscoeReturns said:
Argyreia said:
I'm planning on sterilizing honey/water solution in a glass shot, drop some spores from a print directly out of the pressure cooker and suck the solution into sterile syringes.
Will this work or am I getting into trouble ?

As a side question, when I will squirt out the liquid culture, can I let a bit of mycelium in the syringe, flame the needle and suck sterile honey/water to make it grow back ?


Spores are never sterile. Spores to LC, just like spores to grain, are very likely to bring in contaminant organisms.


:whathesaid:

Maybe even more so likely since much of the bacteria that exists won't move/thrive on agar/solid media and thrives in liquid.

Some bacteria I play with will do absolutely nothing on agar. They take over any amount of liquid in short order though.


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Question everything, it's very responsive.

Culture Storage

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Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns]
    #28464117 -

RoscoeReturns said:
Luckystriketony said:





Too dry. Looks matted. What size tote is it in, and how many holes, what size holes. Have you misted at all?


Monotub 20 litres, 1,5l spawn + 4l coco/verm, 1.7l of boiled water for pasteurising (now i know should have added more), 4 side bottom holes and 2 high made holes, 1.2' each. Haven't misted for first 10 days because humidity was good, but then drops from lid and walls disappeared, and i started light misting once per every 1-2 days.

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Re: Ask Quick Questions, Get Quick Answers [Re: Luckystriketony]
    #28464128 -

Luckystriketony said:





That growth on the lower right of the top pic looks concerning. I would not act on it yet but watch it carefully for color changes.


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Re: Ask Quick Questions, Get Quick Answers [Re: Luckystriketony] * 2
    #28464164 -

Luckystriketony said:
Monotub 20 litres, 1,5l spawn + 4l coco/verm, 1.7l of boiled water for pasteurising (now i know should have added more), 4 side bottom holes and 2 high made holes, 1.2' each. Haven't misted for first 10 days because humidity was good, but then drops from lid and walls disappeared, and i started light misting once per every 1-2 days.


Yeah. I run 20L tubs quite a bit. I’m using six 3mm holes down low and 10 up top. You’ve got a lot more FAE than I would use in a mono that size. When your sub dried up you misted, but didn’t dial back the FAE enough to keep it moist. Repeated cycles of wet and dry will mat the surface and IME reduce pinning.

You can keep this one around to see what happens if you like, but I wouldn’t expect much. You’re going to want to drastically reduce the amount of air getting in. May also need to rehydrate by bottom watering. Personally I would move on to other things and make adjustments in airflow for future grows. This was a learning experience.


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Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?


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Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns]
    #28464231 -



Hey guys, going to inject this with a questionable spore syringe in search of cultivating spicy mushrooms. Any thoughts? Will report back!


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How would you feel if you hadn't eaten breakfast?

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Re: Ask Quick Questions, Get Quick Answers [Re: FeelFighters] * 2
    #28464235 -

That would be dope, capsaicin laden mushrooms.

I might just pickle some with jalapenos


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: FeelFighters] * 1
    #28464239 -

FeelFighters said:


Hey guys, going to inject this with a questionable spore syringe in search of cultivating spicy mushrooms. Any thoughts? Will report back!


The NEW UNCLE BENS LADIES AND GENTS


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🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


Help US help YOU TEK

2023 Dehydrator TEK

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Re: Ask Quick Questions, Get Quick Answers [Re: Screwup] * 1
    #28464242 -

Let grow some san francisco treats!


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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids] * 1
    #28464247 -

Too bad I just missed burning man, these coulda been a real hit!

:rush:


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How would you feel if you hadn't eaten breakfast?

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Re: Ask Quick Questions, Get Quick Answers [Re: FeelFighters]
    #28464428 -

Is this trich? Didn't start until two days ago and I noticed the sub was bruised blue where condensation was running down the side. Should I touch and see if it bruises?


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🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: Ask Quick Questions, Get Quick Answers [Re: Pnin]
    #28464430 -

No need, that’s mold for sure


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CV+

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Re: Ask Quick Questions, Get Quick Answers [Re: MojaveMyc]
    #28464447 -

MojaveMyc said:
No need, that’s mold for sure


Dang, thought this one was in the clear because the pins had started before any sign of the green. Thanks:fedora:


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Re: Ask Quick Questions, Get Quick Answers [Re: Pnin]
    #28464464 -

Pnin said:
Is this trich? Didn't start until two days ago and I noticed the sub was bruised blue where condensation was running down the side. Should I touch and see if it bruises?



So, is this contamination? Natal Super Strain spawned Aug 29.


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Re: Ask Quick Questions, Get Quick Answers [Re: SnakeEyes212]
    #28464488 -

How long should I let my SAB settle before I start working? I’ve heard everywhere from 5mins to an hour. If I have all my jars and plates preloaded into the SAB then as soon as my hands enter won’t that just stir everything back up?

Edited by Mids (09/10/23 05:43 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: SnakeEyes212]
    #28464493 -

SnakeEyes212 said:



this one looks like a dog winking at me. cutest contamination ever :heart:

those colorful water drops are metabolites and are a sign of contamination


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The most important step a man can take. It's not the first one, is it?
It's the next one. Always the next step, Dalinar.

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Re: Ask Quick Questions, Get Quick Answers [Re: Mids]
    #28464528 -

Mids said:
How long should I let my SAB settle before I start working? I’ve heard everywhere from 5mins to an hour. If I have all my jars and plates preloaded into the SAB then as soon as my hands enter won’t that just stir everything back up?


I wait 15 minutes. Your arms are fine.


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28464639 -

I threw this outside as it's obviously got some contam. These green things were all over my substrate, can anyone tell me what it could be?



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:sporedrop:🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿 :skittles2:


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Re: Ask Quick Questions, Get Quick Answers [Re: thcee] * 2
    #28464653 -

Mold. Looks like aspergillis


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28464661 -

I have some mushrooms that have grown while I was away. Right now there are 5 with the caps flattened and 4 that are larger than 3inches but still have the rounded heads. The rest of the tub is largely empty with a couple small pins(?) that look like they will be popping up soon. The shrooms are golden teachers if that is relevant.

Would it be ok to harvest the ones that the cap has opened? Would that affect the rest of the flush?

Also, there is some black residue on the top of some of the flat caps. Is this most likely spores, or some sort of contam? I have noticed it in some other photos from the site so I'm thinking it might be normal.

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Re: Ask Quick Questions, Get Quick Answers [Re: LaughingBhuddha]
    #28464674 -

It's spores. And no worried harvesting them now


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Shop: Mushroom-Hut Liquid Cultures   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   North Spore Bulk Substrate   MagicBag.co Certified Organic All-In-One Grow Bags   Myyco.com Golden Teacher Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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