dbag2192 said:

This is a great example of what’s happening..this is Jack Frost..the black is rit agar..the blue is nute agar…looks amazing on the rit…transfer it to nute…it starts sectoring and getting height and turning super tomentose

Edit: this particular nute plate is 5G LME, 10G agar, 1G NY, 500 ml distilled water

Edit edit: I’ve used those plates that look like that and they seem to colonize fine, even colonize sub fine. But they don’t fruit and eventually they get trich.

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tryptkaloids said:

I've always felt like using chemical antibiotics just makes it harder to see holes in your technique

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tryptkaloids said:

I've always felt like using chemical antibiotics just makes it harder to see holes in your technique

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tryptkaloids said:

I've always felt like using chemical antibiotics just makes it harder to see holes in your technique

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TheAtlantean said:

dbag2192 said:

This is a great example of what’s happening..this is Jack Frost..the black is rit agar..the blue is nute agar…looks amazing on the rit…transfer it to nute…it starts sectoring and getting height and turning super tomentose

Edit: this particular nute plate is 5G LME, 10G agar, 1G NY, 500 ml distilled water

Edit edit: I’ve used those plates that look like that and they seem to colonize fine, even colonize sub fine. But they don’t fruit and eventually they get trich.

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tryptkaloids said:

I've always felt like using chemical antibiotics just makes it harder to see holes in your technique

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tryptkaloids said:

I've always felt like using chemical antibiotics just makes it harder to see holes in your technique

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Agreed.


Just go back to LME and AGAR

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dbag2192 said:

tryptkaloids said:

I've always felt like using chemical antibiotics just makes it harder to see holes in your technique

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I basically only use it for cleaning up things..when normal transferring isn’t working…I’m new to it..guy I follow shared the recipe so thought I’d try it out…think I just need to go back to water agar to clean them back up or something..I don’t even know…its so confusing

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TheAtlantean said:

dbag2192 said:

tryptkaloids said:

I've always felt like using chemical antibiotics just makes it harder to see holes in your technique

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I basically only use it for cleaning up things..when normal transferring isn’t working…I’m new to it..guy I follow shared the recipe so thought I’d try it out…think I just need to go back to water agar to clean them back up or something..I don’t even know…its so confusing

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It’s really not.

dbag2192 said:

tryptkaloids said:

I've always felt like using chemical antibiotics just makes it harder to see holes in your technique

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I basically only use it for cleaning up things..when normal transferring isn’t working…I’m new to it..guy I follow shared the recipe so thought I’d try it out…think I just need to go back to water agar to clean them back up or something..I don’t even know…its so confusing

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You’ll figure it out man. Just takes some time. Keep trying and come back when you have more questions or want to update us.

Agar is really easy. I used to think it was complicated and hard. And it just makes takes time to train the eyes

dbag2192 said:

Little better of a pic..I suck really bad at taking pics of agar…thought maybe there was a chance tho that someone could see something off the pic

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It looks great man. Honestly.

The trich is coming from somewhere else.

What about your sub? How are you preparing your substrate?

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but maybe

dbag2192 said:

This is a great example of what’s happening..this is Jack Frost..the black is rit agar..the blue is nute agar…looks amazing on the rit…transfer it to nute…it starts sectoring and getting height and turning super tomentose

Edit: this particular nute plate is 5G LME, 10G agar, 1G NY, 500 ml distilled water

Edit edit: I’ve used those plates that look like that and they seem to colonize fine, even colonize sub fine. But they don’t fruit and eventually they get trich.

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Mycolorado said:

But if you put it back on rit it grows fine?

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dbag2192 said:

Literally goes from this to this haha..this one’s riptide

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TheAtlantean said:

dbag2192 said:

Literally goes from this to this haha..this one’s riptide

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