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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Screwup]
    #28370869 -

Screwup said:
Pandaskis said:
Got my first 0 shroom rating too! Moving up in life

:tryingnottodie:


Yeah it’s from that troll old account. He gave me one too.


Same lol.


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V: Who? Who is but the form following the function of what and what I am is a man in a mask.
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28370897 -

Quote:
stroopwafelman said:


Here are the BRF cakes from my water tub. They've been this way for about 1-2 weeks now, already harvested a "flush" of mushrooms from two empty ones and about to get one from the third.
My question is - how come the entire coir cover didn't get colonized, and doesen't seem like it's going to?
Is this contam related? Is it too dry? Should I just leave it alone? Dunk? Throw away?



bump

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Re: Ask Quick Questions, Get Quick Answers [Re: stroopwafelman]
    #28370900 -

If it was mine, as long as I didn't have more waiting to go in there, I'd just let it ride.  During the winter I've had cakes take 3 weeks to start pinning.


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Re: Ask Quick Questions, Get Quick Answers [Re: Janus62]
    #28370904 -

QQ -

When taking a biopsy I find it really tricky to chop out a bit of material and transfer it without it 'flicking' across the SAB or me dropping it.  I'm thinking maybe a stainless steel tweazers, flame sterilised would help - pinch a bit and drop it on the plate.  Is that common practice, or is there any reason why I shouldn't?

Come to think of it, is there any reason why I shouldn't do the same for plate to plate transfers?


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Re: Ask Quick Questions, Get Quick Answers [Re: Janus62] * 1
    #28370910 -

Quote:
Janus62 said:
QQ -

When taking a biopsy I find it really tricky to chop out a bit of material and transfer it without it 'flicking' across the SAB or me dropping it.  I'm thinking maybe a stainless steel tweazers, flame sterilised would help - pinch a bit and drop it on the plate.  Is that common practice, or is there any reason why I shouldn't?

Come to think of it, is there any reason why I shouldn't do the same for plate to plate transfers?



I used tweezers to transfer samples from a BRF puck to water agar a few days ago (first time) in my experience stuff loves sticking to flame sterilized tweezers so be prepared having to use the edge of a petri or whatever you're transferring into to act as a scraper of sorts to get the material off the tweezers.

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Re: Ask Quick Questions, Get Quick Answers [Re: stroopwafelman]
    #28370944 -

On that note, I've been just wetting my tools then wrapping them in foil and PCing them with my agar plates and sometimes grain jars.

Should kill any contams, and then I have a sterile wrapped pimple popper tool I bring into my SAB, where it gets unwrapped.

I don't think that has been a contamination vector, but is that sound? Just something I started doing.


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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #28370986 -

Huskies said:
Ok, so I just fucked up a whole batch of agar plates. Fortunately I had two spare from the last one.

I've been doing them in the Instant Pot, but this time they all came out bacterial, still wrapped in foil.

I did leave them out after pouring the pastyplates to avoid condensation, thinking that any bacteria would be killed in the pc cycle.

Apparently not, even at 35 mins.

Is their a better updated pastyplate/Instant Pot tek? Last batch I just did grainwater from oats boil and agar powder and tbh those were really nice.


Are you venting your instapot? When I have done holy grail containers in mine I do 45 minutes total with 15 of that being venting time.

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #28371061 -

Huskies said:
On that note, I've been just wetting my tools then wrapping them in foil and PCing them with my agar plates and sometimes grain jars.

Should kill any contams, and then I have a sterile wrapped pimple popper tool I bring into my SAB, where it gets unwrapped.

I don't think that has been a contamination vector, but is that sound? Just something I started doing.


If I think of it I like to pc a few scalpels too.


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Re: Ask Quick Questions, Get Quick Answers [Re: thirdeyewild] * 4
    #28371063 -

you dont really need to pc a scalpel for 2hrs tho 30min is plenty. i wrap items in foil and place inside an empty jar while do agar/lc cycles.


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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Ask Quick Questions, Get Quick Answers [Re: mushboy]
    #28371082 -

So after my first shoebox I haven’t been able so grow any mushrooms since. Everything contams or stalls out. My grain, my agar, or my bulk. It there a trick to finding out what’s going on? Is it my LC or everything I do? I would test my LC on agar but that always contams or stalls too. So it’s making troubleshooting difficult

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Re: Ask Quick Questions, Get Quick Answers [Re: Jmyco] * 5
    #28371087 -

:facepalm3: you just laid it all out.

stop using dirty LC.

only the cleanest and best agar cultures go to grain.

you only expand the best and cleanest looking grain jar.

repeat.

post pics of your work and be ready to accept critique.


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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

Edited by mushboy (06/23/23 10:40 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: mushboy]
    #28371099 -

:whathesaid:
I suggest starting from spores on agar, transferring that a few times making sure its clean, and then running it on grain to test or "Proof" your culture.
If it produces fruits take more spores and clones on agar.

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Re: Ask Quick Questions, Get Quick Answers [Re: Jmyco] * 4
    #28371136 -

Jmyco said:
So after my first shoebox I haven’t been able so grow any mushrooms since. Everything contams or stalls out. My grain, my agar, or my bulk. It there a trick to finding out what’s going on? Is it my LC or everything I do? I would test my LC on agar but that always contams or stalls too. So it’s making troubleshooting difficult


What mushboy said, plus this:

When first starting out I advise you resist the urge to buy or use liquid culture for the following reasons:

Don't use LC until you have all of the lead up techniques including the ability to produce reliably clean spawn nailed down. Stay as far from LC as possible until then, otherwise you'll be chasing variables and failures forever.

Each skill builds upon the next.

1. Spores to agar to produce culture; cleaning to produce pure culture, making transfers, and agar to grain.

This step will familiarize you with the fungal life cycle and introduce you to the basics of identification for both contamination and healthy mycelium. During this phase you'll learn how to reliably produce clean cultures and sterilize both grains and media.

Once these skills are mastered, move to skillset #2

2. Grain to grain expansion.

This step expands upon the previous production phase by introducing how to expand your pure culture exponentially. This is a more advanced stage dealing with inoculation techniques and aseptic techniques which will prepare you for the next step:

3. Liquid inoculant/liquid culture.

Liquids will not work for you if you haven't learned to identify pure culture, contamination, or prepare sterile media which was learned way back in step one. Liquids won't work for you if you don't understand/master the aseptic techniques required for expansion.

Walk before you run, otherwise there's too many variables to troubleshoot. Each phase carries it's own set of criteria for troubleshooting problems, if you skip them, you're going to be fucked.

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Re: Ask Quick Questions, Get Quick Answers [Re: Stipe-n Cap] * 2
    #28371138 -

Good post. Thanks.

:goodwork:


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Re: Ask Quick Questions, Get Quick Answers [Re: Stipe-n Cap]
    #28371141 -

Whats the general consensus on what to do once the PC timer hits 2 hours? Turn off heat, then what? Remove jars as soon as pressure drops? Let them cool off inside the pc before removing? Ive been removing them asap, stripping the foil off, and wiping off the bit of water ontop the lid asap.


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Edited by AspectOfTheCreator (06/23/23 11:37 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: AspectOfTheCreator]
    #28371150 -

2hrs hits, I just turn it off and let it cool. Depending on what I'm PCing I do a couple different things.
For my grain jars I let them cool in the PC over night before use. I don't remove the tinfoil or anything at all after that.
Just toss agar in and go.
For an agar vessel or liquid culture I take those out immediately and wait for them to cool in an SAB for use.
This is not how everyone does it, but I've done it like this for a long time.


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Re: Ask Quick Questions, Get Quick Answers [Re: mushhead]
    #28371152 -

Ive noticed theres always a tiny puddle between the lid and foil. Makes me paranoid it will seep through the sfd patch


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Re: Ask Quick Questions, Get Quick Answers [Re: AspectOfTheCreator]
    #28371155 -

You can soak those up as you open your jars for use.
The water between the tinfoil and dish went through the same PC cycle as the rest of it so I would consider it pretty clean although I do soak it up and don't allow it into the jar itself.

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Re: Ask Quick Questions, Get Quick Answers [Re: mushhead]
    #28371157 -

Can the atmosphere inside the pc still be considered sterile after its cooled though? Im having widespread contam issues im trying to nail down. Trying to think of anything i can do differently


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Re: Ask Quick Questions, Get Quick Answers [Re: AspectOfTheCreator] * 1
    #28371160 -

AspectOfTheCreator said:
Whats the general consensus on what to do once the PC timer hits 2 hours? Turn off heat, then what? Remove jars as soon as pressure drops? Let them cool off inside the pc before removing? Ive been removing them asap, stripping the foil off, and wiping off the bit of water ontop the lid asap.


Let the jars cool in the pc until the next day. The pc will be fine until you open it. There's no way to prevent moisture in an environment saturated with pressurized steam. SFDs are hydrophobic.

If you're experiencing widespread contamination it will be due to user error. Go back to the basics, create a checklist until you're familiar enough.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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