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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Tri]
    #28368626 -

I used like 4% Karo, idk if there was a specific tek but it’s probably been like 2 months. Same happened with the previous one as well but this one grew more. Shaking it more definitely helped but it’s still so sparse.
Next I am doing agar-> lc so hopefully that goes better

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Re: Ask Quick Questions, Get Quick Answers [Re: BlueRising]
    #28368657 -

Can i use an aborted pin to clone to agar? Will it fruit or just give me β€œabort genes”? May be a dumb question but im thinking that the genetics could be fine but growing conditions simply caused the abort. Thanks!

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Re: Ask Quick Questions, Get Quick Answers [Re: Chamo]
    #28368662 -

altford78 said:
I remember having a conversation about this, can't remember with who or where though. To sum it up, there is a concern that charcoal just suppresses contaminates rather than killing them. So you just don't see them, but they are there and will pick up once you transfer to a new medium.

Might be bullshit though, do your own research.



Nah contam isn't really suppressed on charcoal agar
I just made 20ish ish plates with charcoal agar with lme/pept/yeast and poured them in my SAB at 120 degrees, popped the lids on and let them cool.

3 days after transferring mycelium from PDA plates to them and they all have zero contams.
HOWEVER
One plate apparently got something in it from the air, because a mold/bacteria culture popped up on an unused plate 3x the size/visibility of the growth on my transfer plates.
I'll try to get a pic of it tonight to give an example


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Edited by MicroMycelium (06/21/23 02:14 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: MicroMycelium]
    #28368844 -

Looking okay here, or am I in trouble?



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Probably an idiot, but I think it's fine

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Re: Ask Quick Questions, Get Quick Answers [Re: PicOdeGuyOh] * 2
    #28368845 -

Boi dat shit look wet as hell


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Re: Ask Quick Questions, Get Quick Answers [Re: Tri]
    #28368846 -

Tri-Polar said:
Good answer, ill probably just hold out till my weekend.

I've had huge trip anxiety since a bad trip at the beginning of 2022, hadn't tripped since and kinda swore it off but made these chocolates and just decided to go for it, was in the mirror peaking laughing and lecturing myself "never be afraid to trip again! You are a trip GOD" like a mantra lmao, watched grand budapest hotel and had an amazing time, so I think I'm just eager to get at it again :grin:
Thanks for the words of wisdom


hell yeah dude!! grand budapest fucking kicks ass. glad you could get back in the game dude, it’s always terrifying coming back to psychedelics after a shitty experience


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Re: Ask Quick Questions, Get Quick Answers [Re: Screwup]
    #28368851 -

Yeah... that was my fear. I'll open it up a lil more and give her a fan


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Probably an idiot, but I think it's fine

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Re: Ask Quick Questions, Get Quick Answers [Re: PicOdeGuyOh]
    #28368852 -

I have this wild feeling you got here from fanning and misting just crack it slightly and leave it be unless you loser the micro droplets.


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Re: Ask Quick Questions, Get Quick Answers [Re: Screwup]
    #28368857 -

I hadn't fanned at all, but have been misting apparently too often


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Probably an idiot, but I think it's fine

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28368865 -

To keep a right sterile technique inside the SAB, it's OK to take off the wrap of the Petri dishes inside it and then rub them with a paper towel soaked in ISO 70%? It's also recommended to rub the jar caps inside the SAB? Or does these actions are not good 'cause of air movement created inside it?

After losing 3 jars out of 4 (agar to grain), I guess that something's wrong with what I do.


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Re: Ask Quick Questions, Get Quick Answers [Re: PicOdeGuyOh] * 1
    #28368902 -

PicOdeGuyOh said:
I hadn't fanned at all, but have been misting apparently too often


Ah then don’t start fanning ever πŸ˜‰


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Re: Ask Quick Questions, Get Quick Answers [Re: Nerex] * 1
    #28368904 -

ISO sanitizes not sterilizes. I don’t rub either with ISO and have no problems. Pics of agar and grains?


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Re: Ask Quick Questions, Get Quick Answers [Re: PicOdeGuyOh]
    #28368911 -

Only mist when necessary. Don’t listen to skrewup. Fanning didn’t cause this problem.

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Re: Ask Quick Questions, Get Quick Answers [Re: Chamo]
    #28368919 -

Yeah, I get mist happy. Just put a "No Touchy!" sticky note on my mister so I think before I act :grin:


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Probably an idiot, but I think it's fine

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Re: Ask Quick Questions, Get Quick Answers [Re: Chamo] * 1
    #28368927 -

Chamo said:
Only mist when necessary. Don’t listen to skrewup. Fanning didn’t cause this problem.


There really is no need to fan. Fanning has only ever dried out my subs.
No I'm not going to fight you on this, you do you babe. :cheers:

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Re: Ask Quick Questions, Get Quick Answers [Re: mushhead]
    #28368928 -

Which would hurt his over saturated substrate how?

To clarify I am not advising him to fan. I simply said it isn’t the problem.

Edited by Chamo (06/21/23 05:06 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Chamo] * 2
    #28368933 -

All he needs to do is allow for regular evaporation to do the job.
Fanning would dehydrate things too quickly.
I'm seriously not going to debate this.

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Re: Ask Quick Questions, Get Quick Answers [Re: mushhead]
    #28368934 -

Then don’t.

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Re: Ask Quick Questions, Get Quick Answers [Re: Chamo] * 3
    #28368944 -

:popcorn:


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Re: Ask Quick Questions, Get Quick Answers [Re: aPurpleCray0n] * 2
    #28368954 -

He’s already back on his bullshit the absolute troll. I ASSUMED, which made me the ass of course, he was fanning and misting.

For clarification chamo is our new troll. Fanning and misting repeatedly which new people tend to do causes issues.


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Edited by Screwup (06/21/23 05:24 PM)

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