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Re: Ask Quick Questions, Get Quick Answers [Re: Luftwaffles90]
    #28332060 -

Luftwaffles90 said:
Can hoogshagenii be spawned to plain coir/ verm or does it need additional nutes?



I´d treat them like panaeolus as grass lovers.
Never grew them though.

        :cookiemonster:


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Re: Ask Quick Questions, Get Quick Answers [Re: Goatrider] * 3
    #28332076 -

Any thoughts on wtf is happening with this shoebox? It's just getting weirder & weirder by the day.

7th day fully colonised, misting whenever it's looking dried out as it has been drying slightly. No casing layer. 1:2 ratio. Z-strain.

Blue bruising from yesterday looks almost green today so I'm wondering if it's fucked or what?


Edited by stockw (05/24/23 09:28 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28332166 -

Nichrome said:
Looks like your spores were clean but not viable (dead). It also appears that your environment is also clean and chance did not land anything that would eat that media upon it. If you want to know if the agar is useless, just lick your finger and touch the media in one of your plates. If stuff grows, the agar is likely fine.

The color of that agar is weird. What kind of agar did you buy?



Light Malt Extract and Peptone Agar Plates


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"Let yourself be silently drawn by the strange pull of what you really love. It will not lead you astray." – Divan-e-Shams

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The Hitchhiker's Guide to the Shroomery

Fruiting Cubes


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Re: Ask Quick Questions, Get Quick Answers [Re: stockw] * 1
    #28332181 -

How is your tub getting air?


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How to post pictures to shroomery TEK

Shroomery Trusted Cultivator And Member YouTube Channels.

“Evey Hammond: Who are you?
V: Who? Who is but the form following the function of what and what I am is a man in a mask.
Evey Hammond: Well I can see that.
V: Of course you can. I'm not questioning your powers of observation I'm merely remarking upon the paradox of asking a masked man who he is”


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Re: Ask Quick Questions, Get Quick Answers [Re: johnukguy]
    #28332203 -

My tubs aren't 'true' shoeboxes as they're still airtight with the latch undone so I've flipped the lid leaving small gaps. There's very little air movement in the room.

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Re: Ask Quick Questions, Get Quick Answers [Re: stockw]
    #28332223 -

I wonder if a little more FAE might help?


--------------------
How to post pictures to shroomery TEK

Shroomery Trusted Cultivator And Member YouTube Channels.

“Evey Hammond: Who are you?
V: Who? Who is but the form following the function of what and what I am is a man in a mask.
Evey Hammond: Well I can see that.
V: Of course you can. I'm not questioning your powers of observation I'm merely remarking upon the paradox of asking a masked man who he is”


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Re: Ask Quick Questions, Get Quick Answers [Re: johnukguy] * 1
    #28332239 -

Yesterday I've poured my first agar dishes and I want to wait to see if this plain agar contam before I inoculate spores in it.

How many days do I need to wait before I can inoculate safely ?

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Re: Ask Quick Questions, Get Quick Answers [Re: enscred1312] * 2
    #28332241 -

Bacteria will show up in a day or two...molds a few days after that.

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Re: Ask Quick Questions, Get Quick Answers [Re: enscred1312] * 1
    #28332255 -

Some bacteria can show up within 12hrs, most will show within 24-48, and most molds will germinate within 48-72hrs.
After that if your plates are clear, use em.


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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122] * 1
    #28332266 -

Proposition 122 said:
Proposition 122 said:



I can't even grow contams!

In my effort to track down why things are not working I reconciled myself to the fact I have to learn agar:rolleyes: To eliminate the possibility of me making bad agar i purchased pre-made agar plates off the internet. I inoculated one with Penis Envy (LC Syringe), another with Golden Teacher (spore syringe), and left the other open to the air for one minute as a control.

Since NOTHING grew, including contams, I suspect the agar is bad/non-existent/corrupt. Would that be the right conclusion? Should I try again with different agar? What next?

EDIT: stored at a consistent 70-degrees, intermittent light.



Bump



I had a very similar problem. I started off using the standard amount of agar; 2 g per 100 mL H2O. Well, turns out when using telephone agar with that recipe the agar is too hard and the only thing that will ever grow is slime. Spores wont germinate on it nor will lots of other contams show up. Once I lowered the agar amount to 1.3 g per 100 mL H2O they worked flawlessly.

The LC syringe still should have grown some myc though. The syringe could be bunk or old.

Id never buy premade anything in this hobby. Cant rely on other people to not mess your grow up. Theyll never put the care and quality control into things that you will. And then after having these things shipped in the mail... ehhh. It's just way more fun making all your own stuff.

But that's just me.


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Edited by AspectOfTheCreator (05/24/23 11:02 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: johnukguy] * 2
    #28332280 -

johnukguy said:
I wonder if a little more FAE might help?



I'm hoping I've not put effort into this culture that doesn't like fruiting. It originated from a plate pin so idk maybe it likes being starved of oxygen the kinky bastard.

Time will tell!

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Re: Ask Quick Questions, Get Quick Answers [Re: stockw]
    #28332292 -

I'd like advice on what to look for to know if my tubs are dialed in. They have condensation on the sides, not too much, dryer around the holes with polyfill.  No droplets on the surface.  Worth misting before pinning?

What about surface conditions? I hate doing liners, but in the past without them I'd get side pins/under pins, and I would like to avoid that.

Also, I have found that my swabs are not taking to my agar plates.

I pressure cook moist q-tips wrapped in foil, then swab the print under my sab, get it somewhat black from doing a portion of the print, and swab it on my plates, rubbing it in, even in two different corners.

Hasn't been working as well as it used to. I remember hearing that I need just a bit, but maybe I should get my q-tip fully saturated with spores?


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I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

Edited by Huskies (05/24/23 11:18 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #28332296 -

Pics please.


--------------------
How to post pictures to shroomery TEK

Shroomery Trusted Cultivator And Member YouTube Channels.

“Evey Hammond: Who are you?
V: Who? Who is but the form following the function of what and what I am is a man in a mask.
Evey Hammond: Well I can see that.
V: Of course you can. I'm not questioning your powers of observation I'm merely remarking upon the paradox of asking a masked man who he is”


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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #28332304 -

Huskies said:
I'd like advice on what to look for to know if my tubs are dialed in. They have condensation on the sides, not too much, dryer around the holes with polyfill.  No droplets on the surface.  Worth misting before pinning?

What about surface conditions? I hate doing liners, but in the past without them I'd get side pins/under pins, and I would like to avoid that.

Also, I have found that my swabs are not taking to my agar plates.

I pressure cook moist q-tips wrapped in foil, then swab the print under my sab, get it somewhat black from doing a portion of the print, and swab it on my plates, rubbing it in, even in two different corners.

Hasn't been working as well as it used to. I remember hearing that I need just a bit, but maybe I should get my q-tip fully saturated with spores?



If your tub is fully colonized, there should be beads of moisture on the mycelium. If there's not, you're not dialed in and may need to supplemental mist until you fix your airflow properly. Try tightening up the poly a bit.

As for sidepins, without liners good surface conditions and a well packed down sub will be your best way to avoid them. Also, I made the mistake of flexing the sides of tubs when i picked them up, which would always create a microclimate and give me side pins for the longest time.

For the agar, what is your recipe? Likely you agar is too hard. I'd give cutting off the tip of the swab and leaving it in the dish a shot as well.


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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.

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Re: Ask Quick Questions, Get Quick Answers [Re: Way]
    #28332323 -

These aren't fully colonized yet, but It's been a while since my tubs where 100% white. Could be this coir brand (canna coco), but it was at field capacity, but more droplets vs one stream. Perhaps it is just on the drier side and I  need to adjust?











The driest one is the one with medical tape, double sided, which I remember doing succesfully in the past. Perhaps it needs less?

On another note, maybe I'm using too much coir. These tubs were 3:1 per qt. I see here that this shoebox tek is just 1.5:1, perhaps that is a better ratio if I feel like my coir has not been colonizing 100%?

And for the agar, I'm using the regular pasty plate recipe with powdered telephone brand agar which has always worked in the past.  Perhaps if they are too hard, I should decrease the amount of agar powder? I normally do a double recipe, although the potato flakes I find now are butter herb flavored (which has worked in the past as well). Maybe I should do just grain water agar next time?


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

Edited by Huskies (05/24/23 11:53 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #28332357 -

Huskies said:
These aren't fully colonized yet, but It's been a while since my tubs where 100% white. Could be this coir brand (canna coco), but it was at field capacity, but more droplets vs one stream. Perhaps it is just on the drier side and I  need to adjust?











The driest one is the one with medical tape, double sided, which I remember doing succesfully in the past. Perhaps it needs less?

On another note, maybe I'm using too much coir. These tubs were 3:1 per qt. I see here that this shoebox tek is just 1.5:1, perhaps that is a better ratio if I feel like my coir has not been colonizing 100%?

And for the agar, I'm using the regular pasty plate recipe with powdered telephone brand agar which has always worked in the past.  Perhaps if they are too hard, I should decrease the amount of agar powder? I normally do a double recipe, although the potato flakes I find now are butter herb flavored (which has worked in the past as well). Maybe I should do just grain water agar next time?



That surface is nowhere near being colonized enough for you to be worrying about surface conditions. The surface literally doesn't hold beads until more mycelium pokes through.

Don't mess with them until they colonize more. You'll just be adjusting stuff that you can't see the effects of properly.

The ratio is fine. I've never paid attention to my ratio but it is somewhere between 1:3 and 1:6 generally.

Agar wise, if you've had success in the past with those flakes idk why you would need to change it. The amount of agar you use though can determine if the agar is better suited for transfers or spore drops though. I'd start with just decreasing how much agar you put in from around 2% to like 1.5%. If that doesn't help, you can try the standard LME recipe.


--------------------

That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.

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Re: Ask Quick Questions, Get Quick Answers [Re: Way]
    #28332397 -

^ Cool, thanks man. These are smaller Dollarama tubs, 21qts for 4 bucks, best deal I can find.

I think I'm down to do closer to a 1:1 ratio, so this way I could do 6qts total.

Has there been a change to ideal sub depth? Is it still 3in?


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28332401 -

Hello to all

I'm a beginner, in my PF tek jars there is some doubts and i wanna ask you people

First question, first jar

On one side of the jar i have this. I have big jar (i know i know, shouldn't done that) and this is quite small part compare to the rest of the jar.

Rest of the jar looks good



This is second pic that shows pins forming on one side



I read about sectoring in contamination thread, even though i don't see that light brown line and i don't know is this sectoring at all. Refering to first picture regards that.
You guys know better.

It has been fully colonized in the last 24 hours.

What should i do, top fruit it or birth it and the rest that follows?
Should i wait for full consolidation? Those pins are quite big.

SECOND q, second jar

On this one i have same issue, only it's not fully colonised, has some little work to do on the bottom.
Again has small amount IMHO sectoring on one side, rest is good.
Doesn't pin.



Bottom also has some borders and inside the borders is yellowish, you can't see that on picture, had problems with a light.
7 o'clock is a small uncolonnised part




Again, what should i do, top fruit, birth it? Wait for fully consolidate?

Thank you in advance

Edited by killreflection (05/24/23 01:10 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: killreflection]
    #28332428 -

I recently started what could be considered an oversized cake and built a thought-to-be automated setup with a humidifier and fan on automated cycles...

Unfortunately the humidifier got stuck "on" for hours overnight and essentially soaked the entire cake. puddles of water pooling, drenched mycelium top (think watery cottage cheese), and a massive amount of baby aborts and dead pins.

I left it be to dry out and hopefully recover what I could. I was fortunate enough to get a few fat bloomers but only about 5 in total came out.

My questions are: is there any chance for continued bloomers? Should I dunk the cake to "reset" its hydration state? Could I break and remix the cake to even out the overly saturated parts on top with the otherwise fine parts inside?

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Re: Ask Quick Questions, Get Quick Answers [Re: Cryptic Capz] * 1
    #28332450 -

^Seems like you are using old teks

Check these out.

https://www.shroomery.org/forums/showflat.php/Number/24144021


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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