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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Mycodemus] * 1
    #28326164 -

Toss it asap.


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:heart: πŸ…ƒ πŸ„΄ πŸ„° πŸ„ΌΒ Β  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ :heart:


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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra]
    #28326172 -

Keep the big top blob or no? Will toss asap

Edit, picked the blob but it’s not bruising blue, I suspect oyster contamination like I had before, same spore vial. Oh well. It’s all tossed


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An hour and a half later, I was beginning to feel definite effects
Subjective Effect Index: comprehensive map of all potential psychoactive experiences

Edited by Mycodemus (05/19/23 02:03 PM)

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Re: Get Quick Answers [Re: Yahra]
    #28326195 -

Yahra said:
Should i flip over PF cakes in a water tub on a regular basis so that the bottoms get misted too or just leave them in place?


I flipped my cakes because they started to pin at the bottom.

There seem to be much better conditions at the bottom if you compare the pics.


Before flipping



After flipping



I think it's a good idea to flip in that case.

Guess i misted the tops too much, then.


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:heart: πŸ…ƒ πŸ„΄ πŸ„° πŸ„ΌΒ Β  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ :heart:


Edited by Yahra (05/19/23 01:55 PM)

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Re: Get Quick Answers [Re: Yahra]
    #28326226 -

If you have Petri dishes with lids, why do you need parafilm/plastic wrap?


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An hour and a half later, I was beginning to feel definite effects
Subjective Effect Index: comprehensive map of all potential psychoactive experiences

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Re: Get Quick Answers [Re: Mycodemus] * 2
    #28326230 -

To prevent contamination while allowing gas exchange.


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:heart: πŸ…ƒ πŸ„΄ πŸ„° πŸ„ΌΒ Β  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ :heart:


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Re: Get Quick Answers [Re: Mycodemus]
    #28326236 -

What yahra said,

My parafilm roll was pretty old so it ended up cracking and drying, exposing a tiny hole which pin mold entered the dish. So you can imagine what kind of nasties can appear without it.



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Extinction is forever




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Re: Get Quick Answers [Re: Yahra]
    #28326238 -

Yahra said:
To prevent contamination while allowing gas exchange.[/quote
Yahra said:
To prevent contamination while allowing gas exchange.


Did you have any luck ?


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It is never too late to be what you might have been
Every saint has a past, and every sinner has a future You will face many defeats in life, but never let yourself be defeated shames. 

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Re: Get Quick Answers [Re: Dirtnapz]
    #28326240 -

Dirtnapz said:
What yahra said,

My parafilm roll was pretty old so it ended up cracking and drying, exposing a tiny hole which pin mold entered the dish. So you can imagine what kind of nasties can appear without it.




So is best practice to use both film and the lid? I’m confused.


--------------------


An hour and a half later, I was beginning to feel definite effects
Subjective Effect Index: comprehensive map of all potential psychoactive experiences

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Re: Get Quick Answers [Re: Mycodemus] * 2
    #28326245 -

Yes, good practice to use both.


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Extinction is forever




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Re: Get Quick Answers [Re: Wizard_Shames]
    #28326246 -

The only time i did agar was several years ago.

I tried to clone wild fruits, which were covered in all kinds of nasty contams.

So, no, i never had a good petri dish.


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:heart: πŸ…ƒ πŸ„΄ πŸ„° πŸ„ΌΒ Β  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ :heart:


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Re: Get Quick Answers [Re: Mycodemus] * 1
    #28326288 -

Mycodemus said:
If you have Petri dishes with lids, why do you need parafilm/plastic wrap?


You don't need it. You can leave them unwrapped but you'll have to be very careful handling them to look at them. Waving them around unwrapped can result in contam.


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: Get Quick Answers [Re: Mycodemus] * 2
    #28326290 -

Mycodemus said:
Dirtnapz said:
What yahra said,

My parafilm roll was pretty old so it ended up cracking and drying, exposing a tiny hole which pin mold entered the dish. So you can imagine what kind of nasties can appear without it.




So is best practice to use both film and the lid? I’m confused.


How would you not use the lid? Now I'm confused.


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Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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Re: Get Quick Answers [Re: SirPsycho] * 2
    #28326296 -

SirPsycho said:
Mycodemus said:
If you have Petri dishes with lids, why do you need parafilm/plastic wrap?


You don't need it. You can leave them unwrapped but you'll have to be very careful handling them to look at them. Waving them around unwrapped can result in contam.


There's no way you can handle stacks of unwrapped petris and
a) not get contam
b) not go insane
c) not drop you agar on the floor

:breakingbike:

^^^^That's me trying to handle unwrapped petris, on a good day.


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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Re: Get Quick Answers [Re: altford78]
    #28326304 -

Do you wrap it, then put the lid on, like a sandwich… or put the lid on and wrap over the top and bottom together?


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An hour and a half later, I was beginning to feel definite effects
Subjective Effect Index: comprehensive map of all potential psychoactive experiences

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Re: Get Quick Answers [Re: Mycodemus]
    #28326307 -

The latter.


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:heart: πŸ…ƒ πŸ„΄ πŸ„° πŸ„ΌΒ Β  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ :heart:


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Re: Get Quick Answers [Re: Mycodemus] * 4
    #28326310 -

Mycodemus said:
Do you wrap it, then put the lid on, like a sandwich… or put the lid on and wrap over the top and bottom together?


You close the lid and wrap the edge.

Like this :



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Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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Re: Ask Quick Questions, Get Quick Answers [Re: Bigworm] * 1
    #28326328 -

Quote:
They will stay fuzzy but don't worry it doesn't effect anything.



Thank you! I'm so close! I would to get this far and fuck up somehow.


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Citizen Mycologist:mushroom2:

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Re: Get Quick Answers [Re: altford78]
    #28326342 -

altford78 said:
SirPsycho said:
Mycodemus said:
If you have Petri dishes with lids, why do you need parafilm/plastic wrap?


You don't need it. You can leave them unwrapped but you'll have to be very careful handling them to look at them. Waving them around unwrapped can result in contam.


There's no way you can handle stacks of unwrapped petris and
a) not get contam
b) not go insane
c) not drop you agar on the floor

:breakingbike:

^^^^That's me trying to handle unwrapped petris, on a good day.


I'm gonna have to disagree, like I said you would have to be very careful handling them. My no pour plates get left unwrapped in my drawer before use. I've had a total of 2 plates contam before use.

One I ended up handling a lot and the second I brushed with my hand grabbing a plate from another stack and lifted the lid.


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: Ask Quick Questions, Get Quick Answers [Re: FungiSaves]
    #28326343 -

Quick questions:

1. Is this too much water and if so should I wipe the walls with a paper towel?

2. Should the lid be cracked like it is in the pic or just flipped upside-down evenly on top?

3. Will little cups of vinegar keep fungus gnats away?




--------------------
Citizen Mycologist:mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: FungiSaves]
    #28326389 -

Last question, sorry for having so damn many..

Both tubs have the same thing. Smaller tub colonized to 100% two weeks before the larger but didn't start to pin until 5 days after the larger and th4 shrooms are little white blobs vs having brown caps like the larger tub started with. What is going on? Both came from pe mss to lc to gs.




--------------------
Citizen Mycologist:mushroom2:

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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