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Re: Get Quick Answers [Re: chris77]
    #28321546 -

do light/darkness cycles impact in any way cube mycelium growth on agar?

I'm sure this has been researched but I can't find anything.


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Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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Re: Get Quick Answers [Re: altford78]
    #28321559 -

Does trich/mold always manifest itself during spawn colonization or is it possible that it was "dormant" and only manifested itself 2 weeks after spawning to bulk?

Trying to figure out where I went wrong on a recently contaminated mini tub. Spawn looked clean and subtrate seemed to colonize nicely for 1.5-2 weeks until mold started eating it up. Wondering if it was the coir/handling or something while spawning or if it was the spawn that wasn't clean to begin with.


--------------------
---------------------------

Horsie's Distilled TEKs
Grow log 2023: New beginnings
Homemade malt extract because what you bought was not malt extract but malted barley in some form TEK

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Re: Get Quick Answers [Re: Horsewithnoname]
    #28321697 -

Does anyone have experience with making a mushroom concentrate through alcohol extraction? I got some 190 proof alcohol and wanted to give it a shot. I've been seeing a lot of mixed feelings on the subject when searching.


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Signature text goes here

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Re: Get Quick Answers [Re: NuclearTidalWave] * 1
    #28321710 -

NuclearTidalWave said:
Does anyone have experience with making a mushroom concentrate through alcohol extraction? I got some 190 proof alcohol and wanted to give it a shot. I've been seeing a lot of mixed feelings on the subject when searching.


From what I've read it's a waste of time and alcohol :smile: You'd be better off boiling them in water. The more you let the water evaporate the stronger concentrate you'll get.

But if you do decide to give it a shot, make a post of it so others can learn too :cookiemonster:

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Re: Get Quick Answers *DELETED* [Re: Capt. Ramius]
    #28321728 -

Post deleted by stockw

Reason for deletion: double post

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Re: Get Quick Answers [Re: stockw]
    #28321732 -

stockw said:
Capt. Ramius said:
NuclearTidalWave said:
Does anyone have experience with making a mushroom concentrate through alcohol extraction? I got some 190 proof alcohol and wanted to give it a shot. I've been seeing a lot of mixed feelings on the subject when searching.


From what I've read it's a waste of time and alcohol :smile: You'd be better off boiling them in water. The more you let the water evaporate the stronger concentrate you'll get.

But if you do decide to give it a shot, make a post of it so others can learn too :cookiemonster:


It's certainly not a waste of time. I've used Psilocybin tinctures many many times & they do the trick without any nausea often experienced with eating dried shrooms. As long as your extraction is sound. Discretion is a big factor too if that's an issue.

There's a triple extract guide at the bottom of this page https://mycoyusky.com/mushrooms-as-medicine-part-one/

Edited by stockw (05/16/23 11:38 AM)

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Re: Get Quick Answers [Re: Horsewithnoname]
    #28321736 -

Horsewithnoname said:
Does trich/mold always manifest itself during spawn colonization or is it possible that it was "dormant" and only manifested itself 2 weeks after spawning to bulk?

Trying to figure out where I went wrong on a recently contaminated mini tub. Spawn looked clean and subtrate seemed to colonize nicely for 1.5-2 weeks until mold started eating it up. Wondering if it was the coir/handling or something while spawning or if it was the spawn that wasn't clean to begin with.


It doesn't always manifest itself during spawn colonization. Couple of possibilities to consider...

Your spawn had it and it went unnoticed. Early stage trich can be very hard to see in jars, especially if it isn't at the surface.

Or it could have been the coir / handling as you said. Trich can lay dormant in coir with no signs, and then once it gets a nutrient source (grains), it pops up.


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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.

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Re: Get Quick Answers [Re: Way]
    #28321758 -

Sterilizing some PP5 slime cups in the PC today, was wondering what would be a good way to dry them after the cycle? They're wrapped in foil so I was thinking of throwing them in the oven at 250 for 30 mins or something like that.

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Re: Get Quick Answers [Re: Way] * 1
    #28321856 -

Way said:
Horsewithnoname said:
Does trich/mold always manifest itself during spawn colonization or is it possible that it was "dormant" and only manifested itself 2 weeks after spawning to bulk?

Trying to figure out where I went wrong on a recently contaminated mini tub. Spawn looked clean and subtrate seemed to colonize nicely for 1.5-2 weeks until mold started eating it up. Wondering if it was the coir/handling or something while spawning or if it was the spawn that wasn't clean to begin with.


It doesn't always manifest itself during spawn colonization. Couple of possibilities to consider...

Your spawn had it and it went unnoticed. Early stage trich can be very hard to see in jars, especially if it isn't at the surface.

Or it could have been the coir / handling as you said. Trich can lay dormant in coir with no signs, and then once it gets a nutrient source (grains), it pops up.


On this topic Way, for people that have the latter issue & their spawn is clean, do you think sterilising their Coir is the best way to go? Or would Bucket Tek be sufficient to deal with it?

I'm just thinking about the countless times I've read TC's saying spawning to bulk is a non-sterile practice, which I understand. But cleanliness should still come into play to minimise chances of contamination is what you're saying or?

Edited by stockw (05/16/23 01:15 PM)

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Re: Get Quick Answers [Re: stockw] * 2
    #28321867 -

stockw said:
Way said:
Horsewithnoname said:
Does trich/mold always manifest itself during spawn colonization or is it possible that it was "dormant" and only manifested itself 2 weeks after spawning to bulk?

Trying to figure out where I went wrong on a recently contaminated mini tub. Spawn looked clean and subtrate seemed to colonize nicely for 1.5-2 weeks until mold started eating it up. Wondering if it was the coir/handling or something while spawning or if it was the spawn that wasn't clean to begin with.


It doesn't always manifest itself during spawn colonization. Couple of possibilities to consider...

Your spawn had it and it went unnoticed. Early stage trich can be very hard to see in jars, especially if it isn't at the surface.

Or it could have been the coir / handling as you said. Trich can lay dormant in coir with no signs, and then once it gets a nutrient source (grains), it pops up.


On this topic Way, for people that have the latter issue & their spawn is clean, do you think sterilising their Coir is the best way to go? Or would Bucket Tek be sufficient to deal with it?

I'm just thinking about the countless times I've read TC's saying spawning to bulk is a non-sterile practice, which I understand. But cleanliness should still come into play to minimise chances of contamination is what you're saying or?


Check out this thread. He shows that 130f is when trich starts to die.

He also shows that trich that is in the coir for whatever reason, can remain dormant until grains are introduced.

I still use bucket tek with boiling water. I'd say give it a shot if you think it may be an issue.

As for cleanliness when spawning to bulk, I think common sense stuff such as washing your  hands and maybe wiping down the tub should be sufficient. I don't really do much more and dont have issues. You just don't need to be in SAB or flowhood conditions where you worry about a single stray particle contaminating your shit.


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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.

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Re: Get Quick Answers [Re: Way]
    #28321885 -

Hey friends,

I got distracted with life and let my WBS soak for 48 hours, should I just toss it or is there hope for it maybe if I allow it to dry on some sheets for an extended period?


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Generally Confused

Consistently Enthused




Maza Cluster From My First Harvest

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Re: Ask Quick Questions, Get Quick Answers [Re: Kunta Kente]
    #28321916 -

Kunta Kente said:
Roger. I didn't at first as I was more asking a "is some post break humidity something to just always expect."

Bag 1



Bag 2



The mycelium development is uniform and I don't see any weird patches. The bag 2 pic 1 central grain was always darker from initial boil. Bag 2 picture 2 has a single grain that looks shiney but that's just from the flashlight and touching the bag to it accidentally when moving the bag just now. The dark above the grain line is a shadow not grain or discoloration

Just wondering if humidity buildup in bags (these were on the drier side initially during initial in inoculation and colonization) after the break and shake is typical.

Nocc 4/30 with 7cc clean (agar confirmed) LC, both I believe 3.5lb of oats, break 5/12.


Bump


--------------------
My posts are long winded and dense.
Supplemented coir experiments< Currently ongoing spring 2026.

~Agar~ 1000mL water = 1000g water. use ~1% agar...1% of 1000g is 10g.  For cubes, use between 1-2% nutes (10-20g). For pans use 0.5% nutes (5g).

Everything I have typed is purely fiction, and is purely for personal amusement.
All images I've posted are not mine and I am simply posting interesting things I found on the Google.

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Re: Ask Quick Questions, Get Quick Answers [Re: Kunta Kente]
    #28321926 -

Kunta Kente said:
Kunta Kente said:
Roger. I didn't at first as I was more asking a "is some post break humidity something to just always expect."

Bag 1



Bag 2



The mycelium development is uniform and I don't see any weird patches. The bag 2 pic 1 central grain was always darker from initial boil. Bag 2 picture 2 has a single grain that looks shiney but that's just from the flashlight and touching the bag to it accidentally when moving the bag just now. The dark above the grain line is a shadow not grain or discoloration

Just wondering if humidity buildup in bags (these were on the drier side initially during initial in inoculation and colonization) after the break and shake is typical.

Nocc 4/30 with 7cc clean (agar confirmed) LC, both I believe 3.5lb of oats, break 5/12.


Bump


I think it's one of those things where it's a scale. Some build up after a shake is natural from the mycelium doing its thing. If you get excessive moisture after it could be indicative of another colony inside.


--------------------

That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.

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Re: Get Quick Answers [Re: Way]
    #28321930 -

Way said:
Horsewithnoname said:
Does trich/mold always manifest itself during spawn colonization or is it possible that it was "dormant" and only manifested itself 2 weeks after spawning to bulk?

Trying to figure out where I went wrong on a recently contaminated mini tub. Spawn looked clean and subtrate seemed to colonize nicely for 1.5-2 weeks until mold started eating it up. Wondering if it was the coir/handling or something while spawning or if it was the spawn that wasn't clean to begin with.


It doesn't always manifest itself during spawn colonization. Couple of possibilities to consider...

Your spawn had it and it went unnoticed. Early stage trich can be very hard to see in jars, especially if it isn't at the surface.

Or it could have been the coir / handling as you said. Trich can lay dormant in coir with no signs, and then once it gets a nutrient source (grains), it pops up.


Thanks for the reply.

I did use boiling water, but no bucket. Just put it on a big metal bowl until it was cold. What I did forget to do was clean up the tub, which held some stuff before using it.

Hmmm not sure which one it may have been. The bottle I did in parallel is starting to look iffy, so maybe I fucked up the inoculation.


--------------------
---------------------------

Horsie's Distilled TEKs
Grow log 2023: New beginnings
Homemade malt extract because what you bought was not malt extract but malted barley in some form TEK

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Re: Get Quick Answers [Re: Horsewithnoname]
    #28321971 -

Hello again,
Does this looks okay for you?
Its my first from spore via agar and lc to fruits- grow in life.

I had puerto rico and fiji spores but they didnt act successfull... so all on every picture is the A-strain

I have 7 cakes in a "not real water tub" with seramis and all are growing... i give them Fae 3-4 times for 30min ( i crack the lid then)

And its npt BRF its r rye cakes...

Now they are in the tub since 11 days, and first pins was saw at day 5 to day 6.

So now its day 5 in fruiting with pins, and 11 days in the tub

Also i have as you can see 2 jars with growing pins at the side and on top. The one jar is in an bottle and the other jar in a plastic bag.
Its just for fun (experimenting) because i was not sure, if one of the jars was maybe contaminated

Here the pictures



Whats your opinion on this?

P.s. i took the one cake out and i saw, that the one shroom his lost his "veile? The thin mushroom skin between head to stem"

So its  sign that hes already near finish? Bcuz he is so small... :frown:

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Re: Get Quick Answers [Re: BirkenSaft] * 1
    #28321988 -

Maybe just follow a tek people have experience with. There are way too many oddities for me to give advice.

"not real water tub"
"seramis"
"rye cakes"
"wires"

:whattefuck2:


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:heart: 🅃 🄴 🄰 🄼   🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿 :heart:


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Re: Get Quick Answers [Re: Yahra]
    #28322001 -

My second attempt of BRF cakes have been going and produced way more than my first attempt. However while browsing the forum I think I realized that I have a vert problem on them and probably had it on my first attempt. It isn't anything serious, I just get a little brown splotching on the stipes.

My cakes are currently dunking for flush two. If i do have vert should I even both to attempt flush two or will the vert just explode in growth making the cakes useless?

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Re: Get Quick Answers [Re: DelianSK13]
    #28322003 -

If you mean green (vert is French), so yes, toss them.

Moldy cakes can't be saved.


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:heart: 🅃 🄴 🄰 🄼   🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿 :heart:


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Re: Get Quick Answers [Re: Yahra]
    #28322005 -

Sorry, I meant Verticillium. I just wasn't sure how to spell it all out :grin:

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Re: Get Quick Answers [Re: Yahra]
    #28322010 -

I make the real watertub tek, when the first flush is over and the cakes get rehydrate.



My question was, how my pictures look like?

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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