Goatrider said:

altford78 said:

germination plate -> 3 wedges -> T1 plate
Or
germination plate -> 1 wedge -> T1 plate

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I usually transfer promising myc to seperate plates,
so always one transfer to each.
Possibly a T1 will go to grains.
Multiple transfers on a plate may rule each other out,
so you want to transfer again then.

Got it, thanks!

altford78 said:

Also, there is no specific thing to look for in a transfer candidate right? Just grab whatever myc has developed the most?

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Just fast moving from the outer edge,
well organized at it´s best.

         

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altford78 said:

JFC I'm going to have so many plates running in parallel.

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Welcome to the hobby!

Yahra said:

Yes.

But unfortunately, this means that your point is invalid.

There are no fruiting conditions, only grow conditions.

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omg, do i need to spell it out for you? i was being nice.

3 mushrooms on a brf cake? and you call that an abundant flush?
There is a BIG difference between growing mushrooms and growing mushrooms. But for the moment you are busy celebrating your successful grow and i will not rain on your parade.

Anyways.. just know that you have merely scratched the surface. Read more on the role of co2 in growing mushrooms or simply repeat what many others are saying . your choice
with cubensis you will get away with almost anything anyways...

This is what happens when you tell noobs that there are no fruiting conditions. Are you all happy now?

MushyMom said:

I’m leaving on a road trip after work so I am hoping to see where this one goes in the next few hours. These 2 pics were taken 18 hours apart
If it’s trich it’s trich. Because trich happens. But still so frustrating.

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Throwaway said:

altford78 said:

JFC I'm going to have so many plates running in parallel.

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Welcome to the hobby!

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When you realize the petris are multiplying exponentially



For germ plates I've been using a 1.3% AA and 0.8% LME mix, should I go for the standard 2%/2% for the transfer plates or keep the low nutrient % until T2?

Yahra said:

You don't need to spell it out.

I know what growing conditions are.

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altford78 said:

For germ plates I've been using a 1.3% AA and 0.8% LME mix, should I go for the standard 2%/2% for the transfer plates or keep the low nutrient % until T2?

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I'd just try and keep your recipe consistent from now on. Doesn't really matter what that recipe is. Just make sure it doesn't vary all over the place or you can't really draw any conclusions on if a strain is faster/healthier looking. I would personally still jump to 2%/2% though but that's what I've been using since forever so maybe I'm kinda biased.

Yahra said:

Wild species are contaminated anyway, it doesn't make a difference if the swaps weren't sterile.

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altford78 said:

For germ plates I've been using a 1.3% AA and 0.8% LME mix, should I go for the standard 2%/2% for the transfer plates or keep the low nutrient % until T2?

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I'd just try and keep your recipe consistent from now on. Doesn't really matter what that recipe is. Just make sure it doesn't vary all over the place or you can't really draw any conclusions on if a strain is faster/healthier looking. I would personally still jump to 2%/2% though but that's what I've been using since forever so maybe I'm kinda biased.

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Sure, I'll give the 2% nutrient a go. I could theoretically do both 1% and 2% runs to compare side by side but I think that's too much fucking around for my extreme noob level. I'll do that later once I get more experience.

The softer/less nutrient agar was for print germination so I guess I don't need that anymore since I'm transferring.

Thanks

djbluntmagic said:

In this case how far would you trim? What constitutes excess?

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Quote:

djbluntmagic said:I have not tried another grain. I was hoping to make this one process work with which I know people have found success, and then start experimenting. What is it about oats that leads to this outcome and makes them unsatisfactory? Do you have a tek you can link for your preferred grain? Would I simply be able to substitute it for oats and follow the rest of the process about the same?

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PBJ710 said:

My suspicion was that they are using mold preventatives on the oats but I have no proof other than the tag on TSC oats showing the fungicide they use.

I basically use Bods grain prep with some minor tuning - rinse wheat, dump wheat in boiling water for 20 minutes, turn off heat and let it steep for 20, then dry for a couple hours on screen table before bagging.

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