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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: AyePlus]
    #28312466 -

When sterilizing/re-heating agar I leave the lids loose so the air can escape and not burst the bottle. However, at some point I do close the lid tight. After that, if I open the lid at room temp the hiss is just air escaping due to positive pressure, right? Does it dip down to negative pressure after venting and suck air in? Does it matter at all?

Just ignore the hiss and move on with life?


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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Re: Ask Quick Questions, Get Quick Answers [Re: capnnautilus]
    #28312472 -

capnnautilus said:
Back again because a big mistake happened.

The mushroom environment got to 105 degrees overnight, currently I am growing tidal wave and I am scared that they are now dead. Yesterday it also got to 110 but I managed to lower it back to 80 before bed. I don't know if the mushrooms are doomed now.


I think they will be ok. If you look at a few of the countrie's climates they grow in naturally they can get pretty high temps. I do think you run the risk of contamination being greater with those temps but a day shouldn't kill them. You may stunt them for a few days too.


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Pastywhyteโ€™s Ez dialed monotubs
My Miraculix Potency Test Results
The Hitchhiker's Guide to the Shroomery
My Museum
REV'S Tested: Dehydration temperature vs potency





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Re: Ask Quick Questions, Get Quick Answers [Re: altford78] * 1
    #28312473 -

You close the lid when removing from the pc and you open it in your SAB or in front of your LFH.

Or you can just leave it loose, especially if you covered it with foil.


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: Ask Quick Questions, Get Quick Answers [Re: SirPsycho]
    #28312474 -

Takes a little more planning/forethought but if youre running a few varieties simulateously and cycling youโ€™ll never want for more spawn.

Master :g2g 1
Jar: 10 jars
1 jar:6 bags+1 jar jar:6 bags and 1 jar:6bags
60 totes per run off one master for a month easy.

Or cold store a few of the first g2g and bust em out as needed

You could run a whole commercial grow for months off one good a2g if you keep it clean all the way through.


--------------------
Learn about breeding

C10โ€™s agar guide
Good surface conditions = Good pinsets
Read more, post less.
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Re: Ask Quick Questions, Get Quick Answers [Re: altford78] * 2
    #28312475 -

altford78 said:
When sterilizing/re-heating agar I leave the lids loose so the air can escape and not burst the bottle. However, at some point I do close the lid tight. After that, if I open the lid at room temp the hiss is just air escaping due to positive pressure, right? Does it dip down to negative pressure after venting and suck air in? Does it matter at all?

Just ignore the hiss and move on with life?


If you close the lid when hot, that hiss is the vacuum equalizing to room pressure. If youโ€™re doing this anywhere but in front of a flow hood, itโ€™s sucking in nasty air.


--------------------

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Re: Ask Quick Questions, Get Quick Answers [Re: Pandaskis]
    #28312493 -

Pandaskis said:
johnukguy said:
The idea that spores interfere with growth or pinning is a myth. You can safely leave the mature fruits until more have caught up, or just harvest them individually. Whichever you prefer.


I have heard certain TC's say that spores dropping increase chances of contam, since there are certain molds that enjoy eating the spores, not sure how true this actually is, or if the mold is rare enough that it doesnt actually matter


Thanks guys, I'll leave them for a couple more days.  I'm not too worried about possible extra contamination as they're from BRF jars.


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๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ

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Re: Ask Quick Questions, Get Quick Answers [Re: AyePlus]
    #28312503 -

SirPsycho said:
AyePlus said:
stockw said:
My LC's seem to keep fucking me despite following the usual safety procedures (testing on agar, looking healthy but still getting bacterial jars) so I've decided to cut them out & just go agar to grain as my petris are rarely bacterial nowadays.

Anyone else do this? Is it viable / a good option for bulk grows? I'm thinking 20 dishes for 40 jars as I've got a healthy & fast growing isolate currently.

Probably also worth mentioning I was using fairly low quality oats (30m boil, dry & 2 hour PC) which I now understand can cause issues due to endospores so I'm now going with better quality wheat for my next try & a much more thorough tek.

Anyone with experience with the above methods, if you've any advice it'd be greatly appreciated.


Never liked LCโ€™s, i a2g then g2g until i have enough spawn. The speed/convenience of LC has always been outweighed by the margin of error for me


G2G is the tits.


AyePlus said:
Takes a little more planning/forethought but if youre running a few varieties simulateously and cycling youโ€™ll never want for more spawn.

Master :g2g 1
Jar: 10 jars
1 jar:6 bags+1 jar jar:6 bags and 1 jar:6bags
60 totes per run off one master for a month easy.

Or cold store a few of the first g2g and bust em out as needed

You could run a whole commercial grow for months off one good a2g if you keep it clean all the way through.


Honestly man, it's done me dirty so many times but I kept at it as I thought I was learning from a mistake each time & would crack it next.

Feeling optimistic about the a2g however & I'll definitely do some g2g included.

Cheers & all the bestest to ye

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Re: Ask Quick Questions, Get Quick Answers [Re: San Pedro Girl] * 1
    #28312543 -

SirPsycho said:
You close the lid when removing from the pc and you open it in your SAB or in front of your LFH.

Or you can just leave it loose, especially if you covered it with foil.


Thanks!

I think best option for me right now is to leave the lid loose. I'll have to take the foil of to reheat it but that should still be ok.


San Pedro Girl said:
If you close the lid when hot, that hiss is the vacuum equalizing to room pressure. If youโ€™re doing this anywhere but in front of a flow hood, itโ€™s sucking in nasty air.


Thanks, I'll just let the lid loose until it drops to room temp.

Just so my smol brain gets this right, when the temp drops inside the sealed bottle, the expanded gas contracts and is at lower pressure that ambient pressure? For some reason it feels like it should be the other way around, that the hiss would be air coming out of the bottle but my thermodynamics is like kindergarden level.


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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Re: Ask Quick Questions, Get Quick Answers [Re: altford78]
    #28312583 -

I want to top fruit a Pf cake which pinned early (maybe too bacterial) in a ziplock bag.

I've read Bod's easy AF cheapest way to get started by yourself no need for a pressure cooker and that tek tells me to leave the cake in the jar and just use a ziplock bag on top.

Why do i have to put it in the jar again? I have a lot of side pins and i want to directly put it in the ziplock bag so harvesting would be easier.

Is this possible? What's the purpose of the jar after birthing the cake?



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:heart: ๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผย ย  ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ :heart:


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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 1
    #28312593 -

Very possible

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Re: Ask Quick Questions, Get Quick Answers [Re: Prolific Koi] * 1
    #28312602 -

Thanks.

The teks are good and work but they don't explain WHY you do stuff.

So i wasn't sure if i can do it without the jar or not.

:utterconfusion:


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:heart: ๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผย ย  ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ :heart:


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Get Quick Answers [Re: Prolific Koi]
    #28312605 -

I am certain I am not a forward thinker regarding this...

I have access to a pasture. How about if I were to inoculate a cow paddy?
Spore drop or LC?? would I be planting a garden that I could return to time and time again?


--------------------
As it turns out I guess I have been cultivating fungus since they gave me a gym locker in third grade...

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Re: Get Quick Answers [Re: WhatEyeFind]
    #28312606 -

This does not work.
See several threads on that topic.


--------------------
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Re: Get Quick Answers [Re: Yahra]
    #28312632 -

Hey all, was looking for some confirmation that my first monotub is fucked.

Followed bods unmodified tub and oat prep.

Primary source of fuck up was probably prepping all jars at once (grains to inoculate + jars for g2g) and keeping the g2g jars in fridge for the 3 weeks while mother jars readied. Will avoid that in the future.

Anyways, 19 days since spawn. No pins.



One corner has looked weird and dry for a long time. Won't take on any water from misting. Noticing very small blue dots near that corner which I imagine is tich but it hasn't grown in 5 days or so. Tub doesn't look green at all in real life, but the photo is perhaps showing it's true colors.



Temp fluctuations are large, 63-78 on a given day. Should even out as the days get warmer but I'm sure that it hasn't helped.

Going to start from scratch and start over but going to watch this tub for a couple more days to see what happens.

Also, if this tub predictively goes to hell, can I use it as nutrients in a raised bed vegetable garden?

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Re: Get Quick Answers [Re: mrbones]
    #28312662 -

mrbones said:
Hey all, was looking for some confirmation that my first monotub is fucked.

Followed bods unmodified tub and oat prep.

Primary source of fuck up was probably prepping all jars at once (grains to inoculate + jars for g2g) and keeping the g2g jars in fridge for the 3 weeks while mother jars readied. Will avoid that in the future.

Anyways, 19 days since spawn. No pins.



One corner has looked weird and dry for a long time. Won't take on any water from misting. Noticing very small blue dots near that corner which I imagine is tich but it hasn't grown in 5 days or so. Tub doesn't look green at all in real life, but the photo is perhaps showing it's true colors.



Temp fluctuations are large, 63-78 on a given day. Should even out as the days get warmer but I'm sure that it hasn't helped.

Going to start from scratch and start over but going to watch this tub for a couple more days to see what happens.

Also, if this tub predictively goes to hell, can I use it as nutrients in a raised bed vegetable garden?


Hey I'm still rather new but I had a similar problem with my recent first grow. What worked for me was laying a couple layers of saran wrap on top of my colonized sub which triggered pinning. You can also case it with some fresh coir/verm for a similar effect. Again, I'm new and I would listen to more experienced members if their opinions or views diverge from mine.


--------------------
Generally Confused

Consistently Enthused




Maza Cluster From My First Harvest

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Re: Get Quick Answers [Re: Yahra]
    #28312684 -

Yahra said:
This does not work.
See several threads on that topic.


I read some of your suggested readings and I am not convinced that you were informed enough to just shoot me down. Does anyone else have any guidance? It seems like a viable substrate in conducive growing conditions should work.

Of course if a mycelium strain has already 'claimed the territory' my introduction will likely not overpower the resident force.

Of course, I am just learning and ask the questions as they come to mind...

Cheers


--------------------
As it turns out I guess I have been cultivating fungus since they gave me a gym locker in third grade...

Edited by WhatEyeFind (05/09/23 02:16 PM)

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Re: Get Quick Answers [Re: WhatEyeFind]
    #28312689 -

I also didn't know how to use this forum correctly and I asked a question on Yahara question as opposed to starting a new line of questioning of my own...


--------------------
As it turns out I guess I have been cultivating fungus since they gave me a gym locker in third grade...

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Re: Get Quick Answers [Re: mrbones]
    #28312691 -

mrbones said:
Hey all, was looking for some confirmation that my first monotub is fucked.

Followed bods unmodified tub and oat prep.

Primary source of fuck up was probably prepping all jars at once (grains to inoculate + jars for g2g) and keeping the g2g jars in fridge for the 3 weeks while mother jars readied. Will avoid that in the future.

Anyways, 19 days since spawn. No pins.



One corner has looked weird and dry for a long time. Won't take on any water from misting. Noticing very small blue dots near that corner which I imagine is tich but it hasn't grown in 5 days or so. Tub doesn't look green at all in real life, but the photo is perhaps showing it's true colors.



Temp fluctuations are large, 63-78 on a given day. Should even out as the days get warmer but I'm sure that it hasn't helped.

Going to start from scratch and start over but going to watch this tub for a couple more days to see what happens.

Also, if this tub predictively goes to hell, can I use it as nutrients in a raised bed vegetable garden?


What is your humidity level?
How is your air exchange?
IMH(A)O it seems like you only likely need to make minor change to encourage fruiting...


--------------------
As it turns out I guess I have been cultivating fungus since they gave me a gym locker in third grade...

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Re: Get Quick Answers [Re: WhatEyeFind]
    #28312693 -

Thereโ€™s a reason why people follow teks, theyโ€™re proven to work but whoโ€™s to say you canโ€™t tweak a tek to your liking. The bag is still providing a similar environment, should be fine and youโ€™re learning something new.

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Re: Get Quick Answers [Re: WhatEyeFind]
    #28312714 -

WhatEyeFind said:
What is your humidity level?
How is your air exchange?
IMH(A)O it seems like you only likely need to make minor change to encourage fruiting...



Thanks for the response!

At the moment the top upside down on the tub. There's no fan or other holes to increase airflow. A couple days ago I tried removing the cover for a couple hours at a time while misting but nothing happened. Humidity seems OK at the moment but it may have been a little dry those days experimenting with taking the top off.





Edit: Eh it might be dry. Been concerned about swamping it. Just gave it a good spray.

Should I try more air again to encourage pinning?

Edited by mrbones (05/09/23 02:52 PM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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