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Re: Ask Quick Questions, Get Quick Answers [Re: AspectOfTheCreator] * 2
    #28310171 -

AspectOfTheCreator said:
How much do you have to let cultures on agar grow out before using it to inoculate grain? Does it even matter as long as it's clean?



Theoretically one strand would work. I use a whole plate in each jar because then they colonize so damn fast :rocket:

Edit: cut up plate, not a puck

Cheers


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Expanding my mind until I can join the collective

Edited by herbstation (05/07/23 01:26 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28310206 -

I made a post but I'll ask here too. I'm growing in minute rice cups, and Leafly basically says to introduce fruiting conditions right after making the cakes. Should I do that? Or try and let the mycelium grow on the top first like you would with a shoebox or monotub


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What seems to be the officer, problem?

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Re: Ask Quick Questions, Get Quick Answers [Re: Buckdog]
    #28310210 -

Can I get an opinion on a couple of agar plates?
#1


#2


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Re: Ask Quick Questions, Get Quick Answers [Re: Buckdog] * 1
    #28310217 -

As in your using pre made rice? Whatever you already read go ahead and forget it all then look for PFTEK Shroomery.


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πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


Help US help YOU TEK

2023 Dehydrator TEK

Edited by Screwup (05/07/23 02:01 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Screwup]
    #28310312 -

T1 seems a little off. Any red flags?
10g agar, 10g lme, 475ml h20 & a pinch of yeast. 
Appreciate teh halp!



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Edited by Atomsplit (05/07/23 03:48 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Atomsplit] * 3
    #28310316 -

Looks ok to me. Is that low nutrient agar? Thats what my plates look like on 1% LMEA



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Re: Ask Quick Questions, Get Quick Answers [Re: AspectOfTheCreator]
    #28310379 -

Is it possible to have increased contam rates if I don’t clean out my Petri dishes at all( with soap or water that is), dump agar in them, and then run them through the PC for 25-30 mins @ 15 PSI with 15-20 min venting?


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πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


Help US help YOU TEK

2023 Dehydrator TEK

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Re: Ask Quick Questions, Get Quick Answers [Re: Screwup]
    #28310461 -

Transferred a crumb of Pan mycelium on PF tek to a grain jar. Obviously I will find out for myself in time, but what are others opinions? Is this doomed to failure or does it have a shot at success? Haven't found too much to read on pf tek to grains.


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Re: Ask Quick Questions Here [Re: AspectOfTheCreator]
    #28310469 -

Question about plating:

1. I had issues with contamination with my oat grain so I plated fresh spores
- I plated my Penis Envy spores which I had (several months old, January 2023) as well as old Golden Teachers which I had (several years old). I plated 6 plates each of the two
- Only 1/6 of the Penis Envy plated. The remaining plates for both the PE and GTs are empty... is this normal? The growing plate looks normal but I am planning to sector and isolate over the next several weeks

- Several weeks ago I made plates. The plates I did not use, I just left in the SAB
- I ended up purchasing new Penis Envy Uncut and Golden Teachers because I thought maybe these won't grow
- Yesterday, I plated PEU and GTs on the remaining plates I left in my SAB. I have no growth but I also think it's unreasonable to think that I will get growth in one day?

- How long are the spore syringes (which I purchased from Sporeworks) good for? Where should I store them?

Thank you

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Re: Ask Quick Questions, Get Quick Answers [Re: AspectOfTheCreator] * 1
    #28310474 -

It depends on whether or not you are confident in your technique. If you had a clean live culture that went in the cake, and assuming the cake remained clean and free from contamination, you could move mycelium from that scenario to grain successfully. Is it likely to be a good method, no. You can move any clean mycelium to any new food source.

I hope it works out for you.


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Question everything, it's very responsive.


Culture Storage

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] * 1
    #28310623 -

I don't yet have a media bottle.   

Until I can get one, what are viable and cheap alternatives?


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How to post pictures to shroomery TEK

Shroomery Trusted Cultivator And Member YouTube Channels.

β€œEvey Hammond: Who are you?
V: Who? Who is but the form following the function of what and what I am is a man in a mask.
Evey Hammond: Well I can see that.
V: Of course you can. I'm not questioning your powers of observation I'm merely remarking upon the paradox of asking a masked man who he is”


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Re: Ask Quick Questions, Get Quick Answers [Re: johnukguy] * 1
    #28310626 -

I used to just use a pint jar.


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Re: Ask Quick Questions, Get Quick Answers [Re: AspectOfTheCreator] * 1
    #28310632 -

I went from no pour wide mouth half pint jars, to media bottle pouring in glass dishes, and now going back to no pours but in pp5 petri dishes while using a stainless steel can petri dish sterilizer.


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Links For Beginners And Beyond:mspoil:

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Re: Ask Quick Questions, Get Quick Answers [Re: johnukguy] * 2
    #28310644 -

johnukguy said:
I don't yet have a media bottle.   

Until I can get one, what are viable and cheap alternatives?



I would say, literally any liquid tight vessel that won’t melt or crack in a PC!


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Re: Ask Quick Questions, Get Quick Answers [Re: San Pedro Girl] * 1
    #28310646 -

Understood but I'm also wanting something that has at least one clear, accurate, useful and visible measurement on it if possible? If this makes sense? Or I'm just overthinking things.


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How to post pictures to shroomery TEK

Shroomery Trusted Cultivator And Member YouTube Channels.

β€œEvey Hammond: Who are you?
V: Who? Who is but the form following the function of what and what I am is a man in a mask.
Evey Hammond: Well I can see that.
V: Of course you can. I'm not questioning your powers of observation I'm merely remarking upon the paradox of asking a masked man who he is”


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Re: Ask Quick Questions, Get Quick Answers [Re: johnukguy] * 2
    #28310653 -

Ye ol' reliable ball quart jar


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Re: Ask Quick Questions, Get Quick Answers [Re: San Pedro Girl]
    #28310654 -

San Pedro Girl said:
crack in a PC!




Mmmmm crack in a PC


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Links For Beginners And Beyond:mspoil:

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Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69]
    #28310745 -

How do you get a drop or two out of a syringe? I pushed gently then about a cc squirted out :blush:

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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122] * 1
    #28310749 -

Proposition 122 said:
How do you get a drop or two out of a syringe? I pushed gently then about a cc squirted out :blush:



2ml pipette with pump.πŸ˜‰


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Re: Ask Quick Questions, Get Quick Answers [Re: San Pedro Girl] * 1
    #28310753 -

Can you eat baby-boomers πŸ˜† lol

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