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nektar61
Just a smear in the shale

Registered: 07/04/20
Posts: 4,607
Loc: Hollywoo

Last seen: 6 hours, 20 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Mycoind]
#27856821 - 07/10/22 01:52 AM (4 years, 3 months ago)

Mycoind said:

This is something that's been sitting for a month and came up on a AA+ plate. Can someone identify this contam?

Maybe yeast. Or penicillin about to turn green. Or anything, but those two spring to mind. The wrinkly center shape really looks like penicillin.

I'd tape it shut, see if it turns green, post a photo, then toss that cup without opening it. Trash it as is. Cups are cheap.
=--=-=-=-=-

Barry nye said:

Rite people I need some advice

I have changed my agar plate recipe from
From potato flake -agar to LM agar

I've had good results with this
https://www.shroomery.org/forums/showflat.php/Number/27551422
and who needs clear plates when you can have black plates, and the black is also a nutrient?

--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (07/10/22 01:59 AM)

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Smellyhobbit
Amateur Wizard

Registered: 04/01/22
Posts: 18,560
Loc: Booger Central
Last seen: 3 hours, 10 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]
#27856971 - 07/10/22 08:32 AM (4 years, 3 months ago)

I came across this thread while looking into my agar situation:

https://www.shroomery.org/forums/showflat.php/Number/26308964

I also looked into the town where I’m living, and it has particularly hard water. Any opinions? Anyone think this might be causing me problems? This person seemed to be having the same problem as me.

--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:

:kodama:

Anyone can get a canopy. But can you get what you want?

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Rotnpins
🤮 Rotten-Pins 🍄

Registered: 01/11/22
Posts: 4,738
Loc: in (front of) the hood
Last seen: 3 years, 9 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]

2
#27856982 - 07/10/22 08:49 AM (4 years, 3 months ago)

FP+ T2

Due to condensation from putting my grow stuff in the garage for a day, it's hard to tell, but looks pretty damn clean to me :rockon:

:rockon:

TW plate pin

--------------------
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Spore to Grain
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Surface Conditions

Edited by Rotnpins (07/10/22 08:55 AM)

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Smellyhobbit
Amateur Wizard

Registered: 04/01/22
Posts: 18,560
Loc: Booger Central
Last seen: 3 hours, 10 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]
#27856985 - 07/10/22 08:57 AM (4 years, 3 months ago)

Sexy

--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:

:kodama:

Anyone can get a canopy. But can you get what you want?

Extras:

wild_aussie
Punk rocker til I die

Registered: 01/05/22
Posts: 1,007
Loc: Skatepark grinding hard
Last seen: 6 months, 28 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: BlueAndOrange]
#27857087 - 07/10/22 11:42 AM (4 years, 3 months ago)

BlueAndOrange said:

wild_aussie said:

sandman420 said:

wild_aussie said:

So a question about activated charcoal. Is there a specific kind of charcoal to use or not to use? I wanted to try charcoal in my agar so I used activated charcoal capsules and did it turn out weird. It kind of separated in PC and even some plates ended up looking like donuts with holes in center
So there's that. Also every one of them that I put anything on went weird AF also.

Any ideas? Oh ya every plate did this but did noncharcoal plates at the same time in the SAB and they came out clean?

I saw someone else post similar pics (to your top pic) once a while ago.

I have no explanation but you must have done something crazy like not mix it at all or used a whole lot or something?

I only use the smallest little bit these days. Works great.

That might be it I put like 12 capsules in 600 ml of media

I had results similar to this. Cutting back on charcoal and stirring it in in a pot on the stove to fully dissolve it gave great results.

Thanks

--------------------
BEWARE OF PICK POCKETS AND LOOSE WOMEN!!
"Life shouldn't be a journey to the grave with the intentions of arriving safely in a well preserved body, but rather skid in broadside in a cloud of smoke throughly used up totally worn out and loudly proclaiming WOW WHAT A RIDE!"
Hunter S. Thompson
"I want to die peacefully like my grandfather in his sleep, not yelling and screaming like his passengers." Bob Monkhouse
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Not Quite Social

Registered: 07/16/10
Posts: 1,418
Loc: Midwest

Re: AGAR ENVY! (Anything and All things agar!)

[Re: wild_aussie]

1
#27857154 - 07/10/22 12:36 PM (4 years, 3 months ago)

Okay, we're going to see how these iffy plates grow on millet.

I'll compare these three jars to the previous seven jars I inoculated with plates of myc that were not so sectored and suspect looking.

Will potentially unhealthy plates yield unhealthy grain spawn, or will it all turn out about the same?

We shall see.

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wild_aussie
Punk rocker til I die

Registered: 01/05/22
Posts: 1,007
Loc: Skatepark grinding hard
Last seen: 6 months, 28 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]
#27857178 - 07/10/22 12:54 PM (4 years, 3 months ago)

How necessary is agar to a lc? I've been reading in the archives and most of them don't mention agar( I know they are old info) one was just karo and water and another was the norm lme and dextrose. When I tried making lc with agar it formed a thin layer of agar(petri dish like) floating on the top. Do I want this? Then when I swirl it breaks up the agar disk and is chunks of agar floating inside. Is this right? What am I doing wrong? Can I just use sterilized soak water and drop a piece of colonized agar into it? Thanks

--------------------
BEWARE OF PICK POCKETS AND LOOSE WOMEN!!
"Life shouldn't be a journey to the grave with the intentions of arriving safely in a well preserved body, but rather skid in broadside in a cloud of smoke throughly used up totally worn out and loudly proclaiming WOW WHAT A RIDE!"
Hunter S. Thompson
"I want to die peacefully like my grandfather in his sleep, not yelling and screaming like his passengers." Bob Monkhouse
" Sticking feathers up your ass does not make you a chicken!" Tyler Durden
"He who makes a beast of himself gets rid the pain of being a man." Samuel Johnson
The noob forum
Crack Plates
Fastfreds media cookbook 60 agar/lc recipes
Water tub fruiting chamber Top layer/Pseudo casing Complete agar resource guide
Trusted teks and links SEED "PETRI" TEK Vent ur PC

Extras:

Smellyhobbit
Amateur Wizard

Registered: 04/01/22
Posts: 18,560
Loc: Booger Central
Last seen: 3 hours, 10 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: wild_aussie]

3
#27857183 - 07/10/22 01:00 PM (4 years, 3 months ago)

You don’t add agar to an LC, you inoculate LC with a clean transfer from an agar culture.

Agar is not an ingredient in broth.

--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:

:kodama:

Anyone can get a canopy. But can you get what you want?

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Guerrilla
Original Asshole

Registered: 01/30/21
Posts: 3,218
Loc: United Kingdom

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Smellyhobbit]
#27857191 - 07/10/22 01:05 PM (4 years, 3 months ago)

Only add agar if you're following this semi-solid liquid culture Tek.

https://www.shroomery.org/forums/showflat.php/Number/26689470

--------------------
Being pissed on does not make you a real man.

...OR DOES IT?

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Wall.E
Bacteria's Bitch

Registered: 06/05/20
Posts: 3,075
Loc: The Axiom

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Smellyhobbit]

1
#27857356 - 07/10/22 03:12 PM (4 years, 3 months ago)

Smellyhobbit said:

You don’t add agar to an LC, you inoculate LC with a clean transfer from an agar culture.

Agar is not an ingredient in broth.

A straight lc, you’re right. But sslc’s are much better in my experience. They coat the grains rather than just pooling at the bottom

--------------------
Mehhh

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Padaone
Mycophage

Registered: 07/03/22
Posts: 68
Last seen: 2 years, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Wall.E]
#27857457 - 07/10/22 04:55 PM (4 years, 3 months ago)

I'm wanting to get into agar, seem to be having success so far but have bought deli cups. Wondering if the holes people drill in them are primarily for equilibrium of pressure vs for GE?

I'm wanting to try pouring these ones to see how it compares with the no pour that I have used up until now.

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cozmyc
gentle modern ape

Registered: 06/20/21
Posts: 2,164
Last seen: 2 months, 14 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Padaone]
#27857459 - 07/10/22 04:57 PM (4 years, 3 months ago)

Padaone said:

I'm wanting to get into agar, seem to be having success so far but have bought deli cups. Wondering if the holes people drill in them are primarily for equilibrium of pressure vs for GE?

I'm wanting to try pouring these ones to see how it compares with the no pour that I have used up until now.

If your kids on the deli cups are PP your golden. I bought some once, the top and bottoms were diff plastics and the lids shrunk. No good.b Yup, I just use a 1/8" bit.

--------------------
You're conscious population 2 stardust
----------------------
Nothing matters so everything does :sunny:

:sunny:

Edited by cozmyc (07/10/22 04:58 PM)

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Padaone
Mycophage

Registered: 07/03/22
Posts: 68
Last seen: 2 years, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: cozmyc]
#27857464 - 07/10/22 05:00 PM (4 years, 3 months ago)

Thanks. So if I am pouring as opposed to no pour theres not a need for the hole then?

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cozmyc
gentle modern ape

Registered: 06/20/21
Posts: 2,164
Last seen: 2 months, 14 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Padaone]
#27857468 - 07/10/22 05:01 PM (4 years, 3 months ago)

Padaone said:

Thanks. So if I am pouring as opposed to no pour theres not a need for the hole then?

Indeed, yes!

--------------------
You're conscious population 2 stardust
----------------------
Nothing matters so everything does :sunny:

:sunny:

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Bigworm

Registered: 03/19/22
Posts: 3,666
Loc: My happy place.

Re: AGAR ENVY! (Anything and All things agar!)

[Re: cozmyc]
#27857638 - 07/10/22 07:00 PM (4 years, 3 months ago)

I'm looking to send one of these to a master slant and grain. Which of these 2 look the best? These are my best 2 of 10. Should I do another transfer before doing a master? These are 2nd transfer MS Tidalwave. The denser one looks like it has knots forming close to the center. I'm trying to cut down on the amount of duplicate cultures I have, so one gets to grow the other disappears.

--------------------
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The Hitchhiker's Guide to the Shroomery
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REV'S Tested: Dehydration temperature vs potency

Edited by Bigworm (07/10/22 07:04 PM)

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TheBlackFien
Stranger

Registered: 07/10/22
Posts: 2
Last seen: 4 years, 3 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Bigworm]
#27857784 - 07/10/22 08:23 PM (4 years, 3 months ago)

Hey Guys!

I have a quick question if anyone can answer real quick please lol. It might sound dumb but I'm cloning my first mushroom with agar, and I'm confused on the process. If I put a raw mushroom on the agar plate wont the mushroom become moldy, and ruin the plate? I watched videos, and everyone is just cutting the inside of the mushroom and leaving it on the plate, but I just don't understand how the plate doesn't become moldy due to the shroom rotting just after a couple days without being dried.

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Smellyhobbit
Amateur Wizard

Registered: 04/01/22
Posts: 18,560
Loc: Booger Central
Last seen: 3 hours, 10 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: TheBlackFien]

3
#27857786 - 07/10/22 08:26 PM (4 years, 3 months ago)

TheBlackFien said:

Hey Guys!

I have a quick question if anyone can answer real quick please lol. It might sound dumb but I'm cloning my first mushroom with agar, and I'm confused on the process. If I put a raw mushroom on the agar plate wont the mushroom become moldy, and ruin the plate? I watched videos, and everyone is just cutting the inside of the mushroom and leaving it on the plate, but I just don't understand how the plate doesn't become moldy due to the shroom rotting just after a couple days without being dried.

That tissue sample doesn’t mold because it doesn’t die. You’re taking internal tissue, which isn’t (as) exposed to bacteria and external contamination and putting it in a clean environment to grow. Just like when you break up mycelium for spawn it doesn’t die and mold, it grows and reconnects. The fruit body tissue will form new mycelium.

--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:

:kodama:

Anyone can get a canopy. But can you get what you want?

Extras:

Boozie
I like beer.

Registered: 02/18/10
Posts: 1,272
Loc: :ↄo⅃

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Bigworm]

2
#27857875 - 07/10/22 09:48 PM (4 years, 3 months ago)

Bigworm said:

I'm looking to send one of these to a master slant and grain. Which of these 2 look the best? These are my best 2 of 10. Should I do another transfer before doing a master? These are 2nd transfer MS Tidalwave. The denser one looks like it has knots forming close to the center. I'm trying to cut down on the amount of duplicate cultures I have, so one gets to grow the other disappears.

The bottom one, without question.

--------------------

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Bigworm

Registered: 03/19/22
Posts: 3,666
Loc: My happy place.

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Boozie]
#27857902 - 07/10/22 10:13 PM (4 years, 3 months ago)

Thanks boozie! That's what I was thinking as well.

--------------------
Pastywhyte’s Ez dialed monotubs
My Miraculix Potency Test Results
The Hitchhiker's Guide to the Shroomery
My Museum
REV'S Tested: Dehydration temperature vs potency

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TheBlackFien
Stranger

Registered: 07/10/22
Posts: 2
Last seen: 4 years, 3 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Smellyhobbit]

1
#27857910 - 07/10/22 10:23 PM (4 years, 3 months ago)

Thank you so much

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