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Re: Ask Quick Questions, Get Quick Answers [Re: StophJS]
    #28929168 -

You can also try to take closeups of some agar plates, as zoomed in as possible while still being clear, including how the mycelium looks from the bottom of the plate. Bacteria can be very well hidden, and with all your cultures coming from the same source, it's not unreasonable to think that they might have the same contamination affecting different cultures. Have you ever tried genetics from any other source?

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Re: Ask Quick Questions, Get Quick Answers [Re: StophJS]
    #28929173 -

Are you living at high altitude? Even if at sea level, personally I would go longer than 90 minutes at 15psi for quarts. Looks like that's already on your list of next steps though. :thumbup:

If you have lids you haven't modified yet, it would be good to try those to rule out the filters as a cause. I've had pretty awful bacterial grains from failed filters (in my case failed adhesion).

Hope someone here can spot whatever is tripping you up.

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon] * 1
    #28929187 -

So far there’s many factors that could be causing failure:

- Sterile technique
- Grain choice
- PC cycle
- Inoculant
- Poor lid modification

I wouldn’t even pick a single one of these to change. I’d fix all of it right away and I bet your problems end.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #28929194 -

I'll try to follow up on the various questions. I'm only at about 900ft above sea level, but we will see if another 30 minutes on the PC helps me out in any case. I have not tried genetics from any other source other than when I injected spores onto my grains as a total novice, which ironically to this day has been my only successful grow because it's the only one where I used premade spawn.

As far as SAB technique, I must admit I flame sterilized only up front. But again, same results when injecting with LC and no discernible difference between any jars. I think I'm cautious about hovering over open jars, and I absolutely try to minimize contact altogether and certainly don't touch the inside of the jars as they're open.

Am I off base in thinking that introducing bacteria alongside the mycelium rather than it being present within all the grains from the outset would probably still result in the mycelium being allowed to grow out a bit while the bacteria and mycelium duke it out?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #28929200 -

Smellyhobbit said:
So far there’s many factors that could be causing failure:

- Sterile technique
- Grain choice
- PC cycle
- Inoculant
- Poor lid modification

I wouldn’t even pick a single one of these to change. I’d fix all of it right away and I bet your problems end.



This is what I have to do because I don't think I would even piss away the money or time on trying to alter one variable at a time at this point. I desperately hope I can get this figured out and not be relegated to buying premade spawn forever. That's a lame way to be fuckin kicked out of this hobby.

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Re: Ask Quick Questions, Get Quick Answers [Re: Seriously_trippin]
    #28929223 -

Seriously_trippin said:
How do i have 15k posts and not know what coir is? Fair question honestly.That's actually an interesting story sort of. Firstly when I registered no company would sell LCs to California so for a majority of that time I just thought I'd never be able to grow :frown:. Plus I was younger and had access to many shroom dealers.

I started here because I found mushrooms near me house, I picked about 10lbs of it and was "drying it" under a ceiling fan. I had been posting on the shroomery about other psychedelic adventures and alot of pill based posts. And someone here saved my life told me that 10lbs of mushrooms I picked was extremely poisonous. I instantly recognized the value of this site as a resource for unintended harm reduction.

Just about a month ago I checked more sites and sponsored vendors kind of out of desperation because I'm so depressed and found that now Lcs can be shipped to Cali :smile: So from that moment on I've been trying to learn all I can.

It is kind of insane I've been a member of a site called "the shroomery" for so long and never learned how to grow shrooms :lol: I just loved the community and whenever I had harm reduction questions for other drugs I always git great advice here but it's never too late to learn and I've been here so long I know to take the advice because I've grown to really really trust this community over the years and honestly I think that's why I've been so successful so far.

Also I think it's worth noting I started this journey 25 days ago lol so I have alot of learning to do



Dude best of luck to ya!

I too just started a couple months ago. First batch I grew were ridiculously weak. I had to travel so took a 5lb actively fruiting block on a 12 hour drive and my hunch is all that stress (myc was BLUE when I got there lol) freaked em out and caused em to be lackluster. All good though, got another batch almost fully colonized now, and it's been fun learning!

Keep us posted.

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Re: Ask Quick Questions, Get Quick Answers [Re: StophJS] * 1
    #28929277 -

StophJS said:
As far as SAB technique, I must admit I flame sterilized only up front. But again, same results when injecting with LC and no discernible difference between any jars. I think I'm cautious about hovering over open jars, and I absolutely try to minimize contact altogether and certainly don't touch the inside of the jars as they're open.

Am I off base in thinking that introducing bacteria alongside the mycelium rather than it being present within all the grains from the outset would probably still result in the mycelium being allowed to grow out a bit while the bacteria and mycelium duke it out?



There’s the biggest red flag right there. You need to sterilize between inoculations. Also you need to make yourself some real lids imo. These SHIPS and patches aren’t doing you any favors.

If your grains are hydrated and theyre in a properly vented PC for 120 minutes then the problem isn’t your grains. I mean you’re not seeing mold. Just bacteria. And considering the lack of sterility in your process I’d say that’s most likely


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 2
    #28929299 -

StophJS said:
Smellyhobbit said:
So far there’s many factors that could be causing failure:

- Sterile technique
- Grain choice
- PC cycle
- Inoculant
- Poor lid modification

I wouldn’t even pick a single one of these to change. I’d fix all of it right away and I bet your problems end.



This is what I have to do because I don't think I would even piss away the money or time on trying to alter one variable at a time at this point. I desperately hope I can get this figured out and not be relegated to buying premade spawn forever. That's a lame way to be fuckin kicked out of this hobby.



Smellyhobbit said:
StophJS said:
As far as SAB technique, I must admit I flame sterilized only up front. But again, same results when injecting with LC and no discernible difference between any jars. I think I'm cautious about hovering over open jars, and I absolutely try to minimize contact altogether and certainly don't touch the inside of the jars as they're open.

Am I off base in thinking that introducing bacteria alongside the mycelium rather than it being present within all the grains from the outset would probably still result in the mycelium being allowed to grow out a bit while the bacteria and mycelium duke it out?



There’s the biggest red flag right there. You need to sterilize between inoculations. Also you need to make yourself some real lids imo. These SHIPS and patches aren’t doing you any favors.

If your grains are hydrated and theyre in a properly vented PC for 120 minutes then the problem isn’t your grains. I mean you’re not seeing mold. Just bacteria. And considering the lack of sterility in your process I’d say that’s most likely



:hereyougo:


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: Ask Quick Questions, Get Quick Answers [Re: Ben Phartin]
    #28929322 -

Ben Phartin said:
Magspy said:


Hi, new grower here, just checking to make sure that everything is going fine with my grow. I thought it was fully colonised so I misted it and moved it to a cooler more well lit environment, but it's been about a week and a half now and it hasn't really changed at all. The images above are pretty much exactly how it looked a week ago.



Thats a bag, dont open or mist it anymore. Close it up, throw it somewhere and let it do its thing. As soon as you put a bag in fruiting conditions dont touch it. Be patient and leave it alone. Lights really dont matter. Keep the temps close to 75.



Got it, I’ll do that, thanks for the response! Just been a bit worried I’d done something wrong since it hasn’t changed.

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Re: Ask Quick Questions, Get Quick Answers [Re: hamloaf]
    #28929399 -

"Properly Vented" "Make sure you Vent your PC"

As someone probably a week or so away from his first PC Cycle, I could use some clarification this subject. I see it a lot but I'm not sure I've ever read details.

I understand that the intention is to keep the PC at a set psi for a set time, but venting would lower the psi, wouldn't it?

so.. why? when? how many times?

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Re: Ask Quick Questions, Get Quick Answers [Re: Azhiaaziam] * 2
    #28929403 -

Azhiaaziam said:
"Properly Vented" "Make sure you Vent your PC"

As someone probably a week or so away from his first PC Cycle, I could use some clarification this subject. I see it a lot but I'm not sure I've ever read details.

I understand that the intention is to keep the PC at a set psi for a set time, but venting would lower the psi, wouldn't it?

so.. why? when? how many times?



You 'vent' the PC by leaving the pressure regulator off while the PC comes up to temp, and until the only thing being expelled from the pressure relief vent is steam.

The goal is to remove all the air in the PC and replace it with steam before sealing the PC and increasing the pressure. Any air left in the PC will dramatically lower the temperature even at high psi, leading to incomplete sterilization.

Common recommendation is to leave the cap off for ~10 minutes at the beginning, but it sometimes takes mine 20ish minutes to completely vent all the air and only produce steam.

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Re: Ask Quick Questions, Get Quick Answers [Re: notthatsorry]
    #28929411 -

notthatsorry said:
Azhiaaziam said:
"Properly Vented" "Make sure you Vent your PC"

As someone probably a week or so away from his first PC Cycle, I could use some clarification this subject. I see it a lot but I'm not sure I've ever read details.

I understand that the intention is to keep the PC at a set psi for a set time, but venting would lower the psi, wouldn't it?

so.. why? when? how many times?



You 'vent' the PC by leaving the pressure regulator off while the PC comes up to temp, and until the only thing being expelled from the pressure relief vent is steam.

The goal is to remove all the air in the PC and replace it with steam before sealing the PC and increasing the pressure. Any air left in the PC will dramatically lower the temperature even at high psi, leading to incomplete sterilization.

Common recommendation is to leave the cap off for ~10 minutes at the beginning, but it sometimes takes mine 20ish minutes to completely vent all the air and only produce steam.




Excellent.  Thank you!

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28929438 -

Smellyhobbit said:
StophJS said:
As far as SAB technique, I must admit I flame sterilized only up front. But again, same results when injecting with LC and no discernible difference between any jars. I think I'm cautious about hovering over open jars, and I absolutely try to minimize contact altogether and certainly don't touch the inside of the jars as they're open.

Am I off base in thinking that introducing bacteria alongside the mycelium rather than it being present within all the grains from the outset would probably still result in the mycelium being allowed to grow out a bit while the bacteria and mycelium duke it out?



There’s the biggest red flag right there. You need to sterilize between inoculations. Also you need to make yourself some real lids imo. These SHIPS and patches aren’t doing you any favors.

If your grains are hydrated and theyre in a properly vented PC for 120 minutes then the problem isn’t your grains. I mean you’re not seeing mold. Just bacteria. And considering the lack of sterility in your process I’d say that’s most likely



I have the metal lids. Would you just drill and polyfill?

I just pulled one of those agar wedges with gummy grains attached out of a jar and dumped it. It smells 100% like fresh cube mycelium and more so when crumbling it in the fingers.

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Re: Ask Quick Questions, Get Quick Answers [Re: StophJS] * 1
    #28929447 -

Smell isn’t a reliable indicator. Don’t worry about testing for smell.

I thought you had plastic 1 piece lids? You need to follow Benson’s lid mod writeup. It’s the best contemporary post on the subject.

If you want to completely remove lids as a variable, make those. Use real materials, not polyfill or stickers.

The single piece plastic RTV’d filter disk lids are basically un-fuckup-able. They outmatch everything in ease of use and effectiveness.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28929449 -

Thanks for the tip on the tek. What I meant is that I ALSO have the metal lids which are as of yet unmodified. The jars came with both. The kids I've used so far are plastic and have SHIP and .3 micron SFD.

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Re: Ask Quick Questions, Get Quick Answers [Re: StophJS]
    #28929453 -

Can I use 2 tables put together to put my ffu on? Is the slight gap between them going to cause issues?


Getting a 4x2 ffu and not finding a ton of tables with the dimensions I want, but can put 2 steel tables together for perfection

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Re: Ask Quick Questions, Get Quick Answers [Re: StophJS]
    #28929459 -

StophJS said:
Thanks for the tip on the tek. What I meant is that I ALSO have the metal lids which are as of yet unmodified. The jars came with both. The kids I've used so far are plastic and have SHIP and .3 micron SFD.



Personally if you’re having trouble I’d stick to one piece. 2 piece lids are harder even if they’re not technically hard or unreliable. But they’re not as straightforward for someone for whom the process isn’t already fluid.

SFDs or SFD stickers? Because I wouldn’t bother with the stickers and the SHIPs unfortunately have no value for someone interested in agar wedge inoculations. I use 70% LQ and I just pour it from the media bottle into the grains. No point in adding a syringe, needle, and ship as contam vectors.

Toss the SHIPs or put them in a drawer if that feels wasteful. They’re not useful.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: mishro]
    #28929473 -

mishro said:
Can I use 2 tables put together to put my ffu on? Is the slight gap between them going to cause issues?


Getting a 4x2 ffu and not finding a ton of tables with the dimensions I want, but can put 2 steel tables together for perfection



I have an old table with a split in the middle for a leaf, all I did was place a strip of packaging tape over it.


--------------------
O son of Kunti, I am the taste of water, the light of the sun and the moon, the syllable om in the Vedic mantras; I am the sound in ether and ability in man.

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Re: Ask Quick Questions, Get Quick Answers [Re: yoosername]
    #28929510 -

Brilliant, Thank you! And thanks home depot for a random deal on steel tables!

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Re: Ask Quick Questions, Get Quick Answers [Re: mishro]
    #28929530 -

Which plates look cleanest for grain spawn? They all seem good to me but I’m still learning on pointing out 🦠 contamination. Thanks in advance for your help

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