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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Post Your New Tools Of The Trade. [Re: SpaceBaby]
    #27611443 -

SpaceBaby said:
LOL dude speccifically says read me cover to cover :rolleyes:
i can no longer even read a complete chapter of a book. because, iphone ipad imac macbook



I'll be honest, I was a bit disappointed in the book.  While there is some good info in there, there's a fair portion of woo as well.  It's not the best compilation of information, but it is a really cool book none the less.


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🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Post Your New Tools Of The Trade. [Re: Mr Piggy]
    #27612664 -

bongoman said:
Got a bunch of these — do you think they might work for longer term storage of liquid culture?

Or maybe as slants? They are 50ml.




As long as they're sterile. The problem with a lot of plastic tubes is that the tubes are autoclaveable but the lids aren't. PC one tube to test test see what happens. If they're sterile and from a reputable source, you should be good to go.

I picked up some I'd these 50mL self standing tubes from Cell Treat for the same reason. 50mL distilled water and 100mg LME.




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Re: Post Your New Tools Of The Trade. [Re: Doctor Mario]
    #27612773 -

Quote:
I picked up some I'd these 50mL self standing tubes from Cell Treat for the same reason. 50mL distilled water and 100mg LME.





Are you using them as your standard LC container for short term storage/usage or are you using the tubes also for long term storage of an LC? If yes - how long you are keeping the LCs and at which temps?

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Re: Post Your New Tools Of The Trade. [Re: bongoman]
    #27612899 -

Be careful I got a 50 pack of those from celltreat. The lids were not autoclavable. I tried to do no pour slants.

They are sterile and can be poured.

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Re: Post Your New Tools Of The Trade. [Re: Drboomer]
    #27613207 -

MrTambourine said:
Are you using them as your standard LC container for short term storage/usage or are you using the tubes also for long term storage of an LC? If yes - how long you are keeping the LCs and at which temps?


The plan is to use the LC to inoculate larger containers of SSLC. I don't know how long it will stay good for but I imagine that it would be a very long time. A few weeks ago, I used a jar of SSLC that is almost a year old to inoculate grain. Mycelium had colonized the surface of the SSLC and gone dormant. It kicked off on grain right away like nothing had ever happened. That jar has seen temps anywhere from 50°F-80°F.

Drboomer said:
Be careful I got a 50 pack of those from celltreat. The lids were not autoclavable. I tried to do no pour slants.

They are sterile and can be poured.


I assumed that the lids would melt because Cell Treat's website only mentions that the tubes are autoclaveable and says nothing about the lids. I sterilized 500mL of LME LC and used a pipettor to squeeze 50mL of the LC into those tubes.


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Re: Post Your New Tools Of The Trade. [Re: Doctor Mario]
    #27613526 -

For long term storage I would go with just sterile water in the tube and keep them refrigerated.

If you use a sugar broth the culture would excrete the digested sugars and toxify the liquid. (Not good living conditions):lol:


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Re: Post Your New Tools Of The Trade. [Re: Doctor Mario] * 1
    #27613770 -

Doctor Mario said:
MrTambourine said:
Are you using them as your standard LC container for short term storage/usage or are you using the tubes also for long term storage of an LC? If yes - how long you are keeping the LCs and at which temps?


The plan is to use the LC to inoculate larger containers of SSLC. I don't know how long it will stay good for but I imagine that it would be a very long time. A few weeks ago, I used a jar of SSLC that is almost a year old to inoculate grain. Mycelium had colonized the surface of the SSLC and gone dormant. It kicked off on grain right away like nothing had ever happened. That jar has seen temps anywhere from 50°F-80°F.

Drboomer said:
Be careful I got a 50 pack of those from celltreat. The lids were not autoclavable. I tried to do no pour slants.

They are sterile and can be poured.


I assumed that the lids would melt because Cell Treat's website only mentions that the tubes are autoclaveable and says nothing about the lids. I sterilized 500mL of LME LC and used a pipettor to squeeze 50mL of the LC into those tubes.


If you need some autoclaveable slants PM me good sir.

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Re: Post Your New Tools Of The Trade. [Re: Doctor Mario]
    #27613836 -

They actually sell autoclavable lids for the tubes. You just have to order them separately.

They work well and come in bags of 50

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Re: Post Your New Tools Of The Trade. [Re: Drboomer]
    #27613871 -

micelio said:
If you use a sugar broth the culture would excrete the digested sugars and toxify the liquid. (Not good living conditions):lol:


Can you clarify this statement for me please, or provide a source?

As far as I'm aware, hyhae do not produce toxic (toxic to itself) metabolic waste products other than c02, and an array of secondary metabolites that are utilized for defense and "digestion".

Consider that slants are long term storage and contain nutrients from complex carbs (sugars).

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Re: Post Your New Tools Of The Trade. [Re: Stipe-n Cap] * 2
    #27613962 -

Okay, laugh if you like. My son thought I was hilarious with these on. I think they are great.


https://smile.amazon.com/dp/B076Q22RHW/

I originally got them for my wife for model building using the low magnification lenses. I like the high magnification for looking at plates. They also help for taking close up pictures with a phone.

PE plate | 20x lens | 25x lens


Lions Mane | 25x lens | 25x with side light


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Post Your New Tools Of The Trade. [Re: ReverendMyc] * 4
    #27613993 -

:bubbles:


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:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: Post Your New Tools Of The Trade. [Re: LotKid]
    #27614047 -

I'll look for It tonight. I found the write up in the Mycopia forum over a year ago.

The subject was long term culture storage (up to ten years) in sterile water in tubes.


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Re: Post Your New Tools Of The Trade. [Re: micelio]
    #27614082 -

https://mycotopia.net/topic/99685-reviving-h20-suspended-mycelium/
Think that was needles, 2-4 year old cultures. Gourmets.

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Re: Post Your New Tools Of The Trade. [Re: micelio]
    #27614092 -

Got a few more links to PDFs on the topic of distilled water storage and other methods.

https://www.fpl.fs.fed.us/documnts/pdf1994/burds94a.pdf

https://www.hardydiagnostics.com/wp-content/uploads/2016/05/Saving-fungal-Cultures-Sterile-Water-By-Harris.pdf

https://docksci.com/preservation-of-live-cultures-of-basidiomycetes-recent-methods_5b00e351d64ab2d6e0d927a1.html


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🍄PANAEOLUS IN MONOTUBS🍄
🍄OFFICIAL SEMILANCEATA THREAD🍄



⭐Baba's Write Ups and Link Collection⭐

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Re: Post Your New Tools Of The Trade. [Re: Baba Yaga]
    #27614122 -

Every plain corn syrup liquid culture I’ve made has lasted at least two years in the fridge.  I think my oyster is goin on five years old now.


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Asante’s woodlover patch
Moricz cellar
D3’s agar tek
A woodlover genetic theory
holofracatl's Woodlover grows
Tweeq's woodlover grow
Liloldme's grow

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Re: Post Your New Tools Of The Trade. [Re: micelio]
    #27614164 -

Yes I'm aware of long term storage methods, I was curious about your statement regarding the byproducts produced by the presence of sugar that harms the mycelium.

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Re: Post Your New Tools Of The Trade. [Re: Stipe-n Cap]
    #27614435 -

I can't find the particular write-up So I would say, I'm bad...

Right now I'm not finding It.. The file went south when my hard drive went south..

The write-up was on five and up to up to ten years in vacutainers using sterile water for culture long term storage.

In the write-up I remember how they would use a syringe and needle for putting a vacuum in the tube.

They definitely wrote on any kind of sugar broth would not be inducive for long term storage because of what they excrete.

Excrete: of a living organism or cell) separate and expel as waste (a substance, especially a product of metabolism).

When I think of long term storage In sterile water I think of five seven ten years.

Why Is it you never hear of lc. storage for five or more years?

I'm not a trusted cultivator or a mycologist, So I don't totally understand allot of this..


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Ticaretin Yeni Araçlarını Yayınlayın. [Re: pacmanbreed]
    #27615402 -

pacmanbreed said:
Van brother i swear by this diy mini ozone generator of mine inside a sab. Working so far 1 contam out of 160 dishes in a month. Im now testing it in super dry sab work.
Only needs a glass pipe, alum foil, nichrome wire & ccfl drive.





Hi buddy.
While researching about plasma sterilizers, I saw that you were interested in this topic.

Did you post a thread about H2O2 plasma sterilizer?.

Can you share your knowledge about this technique?

please open a topic.
it would be very exciting

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Re: Ticaretin Yeni Araçlarını Yayınlayın. [Re: cekoxa]
    #27618847 -

Crosslock tweezers (thanks smartattck) for fahts gandalf swabs that came in pieces. Unfortunately the swabs broke near the swab tip. Boooooo!



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Re: Ticaretin Yeni Araçlarını Yayınlayın. [Re: hamloaf]
    #27618855 -

We don't change the threads names, do we?


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SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Shop: North Spore Injection Grain Bag   Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Sporeworks.EU Spores for European Microscopy

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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