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nektar61
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Re: AGAR ENVY! (Anything and All things agar!) *DELETED*

[Re: Southerner]
#27580931 - 12/14/21 11:15 AM (4 years, 10 months ago)

Post deleted by nektar61

Reason for deletion: misunderstood post.

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Southerner
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: nektar61]
#27580933 - 12/14/21 11:21 AM (4 years, 10 months ago)

That’s one massive ball of pleurotus mycelium in a liquid culture. I’m saying if .1% can create that much growth doesn’t 2% or 20x more nutrients for agar seem overkill? But maybe using in addition to agar would require more to disperse evenly and have nutrients available throughout where as with LC we can swirl to make contact. Just spitballing.

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m0n0id

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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Southerner]
#27580938 - 12/14/21 11:32 AM (4 years, 10 months ago)

My guess at the reason for factor of ~10 diff in nutrients between typical agar and LC recipes is that in LC the nutrients are much more available than agar. LC is a 3D environment and nutrients can diffuse evenly through the liquid, where in agar nutrients are fixed in place by agar and the grown happens largely on a 2D plane so many nutrients are 'locked' in the body of the agar.

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sandman420
Saint PP

Registered: 06/17/04
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Southerner]
#27580941 - 12/14/21 11:33 AM (4 years, 10 months ago)

Probably has to do with it growing inside of vs on the surface. You can use less than 2% though, I see a lot of people are using around 1-1.5% recipes of MEA.

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A.k.a
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: sandman420]

3
#27581022 - 12/14/21 12:34 PM (4 years, 10 months ago)

I had no idea people were straining agar.

Using lme I only ever get sediment if I didn’t heat it enough.

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Brian Jones
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: aNewBeginning]
#27581043 - 12/14/21 12:50 PM (4 years, 10 months ago)

aNewBeginning said:

Thanks. Yes, I meant LME/Yeast sediment

Coffee filters work, but once I switched to LME it's never needed filtering.

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nektar61
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Brian Jones]
#27582246 - 12/15/21 11:57 AM (4 years, 10 months ago)

Maza T3. Sometimes I think rhizo looks like a bug at this stage.

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Drboomer
The lord magnificent

Registered: 09/22/19
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: nektar61]
#27582690 - 12/15/21 05:18 PM (4 years, 10 months ago)

Not trying to be insulting at all bud but is that smeared consistency just finger oils on the container or is your agar surface bacterial?

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Big_Dub
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Re: AGAR ENVY! (Anything and All things agar!)

1
#27582727 - 12/15/21 06:12 PM (4 years, 10 months ago)

Looks like scratched plastic. Maybe a reusable petri

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bigfootscreepyuncl
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Big_Dub]
#27582741 - 12/15/21 06:28 PM (4 years, 10 months ago)

Quote:

Big_Dub

<< you know what it is :cool:

:cool:

agar pic tax:

PFRS germ plate that needs a damn transfer

T2 plate that's lookin alright

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Yeatster
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Registered: 08/07/21
Posts: 8,281

Re: AGAR ENVY! (Anything and All things agar!)

[Re: bigfootscreepyuncl]

1
#27582817 - 12/15/21 07:49 PM (4 years, 10 months ago)

I would like to use 1 small wedge in 2 separate LC's. I like one for the uniformity yet I like the other for aggressiveness.

Could someone give me an opinion on which is a better candidate? Or should I do 1 of each?

Please and thank you!

Edited by Yeatster (11/06/22 02:39 PM)

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Big_Dub
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: bigfootscreepyuncl]
#27582845 - 12/15/21 08:17 PM (4 years, 10 months ago)

bigfootscreepyuncl said:

Quote:

Big_Dub

<< you know what it is

agar pic tax:

PFRS germ plate that needs a damn transfer

T2 plate that's lookin alright

Nice! That lpeu culture is awesome

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nektar61
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Drboomer]
#27582899 - 12/15/21 08:58 PM (4 years, 10 months ago)

Drboomer said:

Not trying to be insulting at all bud but is that smeared consistency just finger oils on the container or is your agar surface bacterial?

haha. Tape residue on the lid. After I pour plates, I tape them in stacks, and leave them a week to see if any are contammed. This one was on the top.

5 oclock is just agar that had a little ripple from moving it while still cooling to put tape on.

This is very close to very bright light, and I increased contrast to see the mycelium better.

I'll post a pic of this later when I open it up.

I wouldn't be insulted to have someone point out contam I missed.

--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (12/15/21 09:12 PM)

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Yeatster
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Posts: 8,281

Re: AGAR ENVY! (Anything and All things agar!)

1
#27582955 - 12/15/21 09:53 PM (4 years, 10 months ago)

Yeatster said:

I would like to use 1 small wedge in 2 separate LC's. I like one for the uniformity yet I like the other for aggressiveness.

Could someone give me an opinion on which is a better candidate? Or should I do 1 of each?

Please and thank you!

I decided on the 2nd pic. 1:30 and 4:30. I'm still interested in opinions tho, if anyone cares to chime in.

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Doctor Mario
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Registered: 08/07/20
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Yeatster]
#27583025 - 12/15/21 11:06 PM (4 years, 10 months ago)

It all looks healthy to me. How much LC are you making?

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Yeatster
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Doctor Mario]
#27583144 - 12/16/21 02:27 AM (4 years, 10 months ago)

Doctor Mario said:

It all looks healthy to me. How much LC are you making?

Thank you! That's a relief. I made (2) 500ml media bottles with 250ml of LC each. 1 of them I did 1g LME/0250ml. The other I did 2.5g LME/250ml. I have the heavier nute LC on a magnetic stirplate with a stirbar @100 running constant. The lighter LC left to rest.

I read Mushboys LC tek a couple years ago and started one but had to pull the plug. I read it again today and decided from the comments I will do 1 his way (1g/250ml) and 1 with a commentary idea of more LME and a stir plate, for faster growth. Should be fun to compare either way.

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Doctor Mario
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Yeatster]
#27583309 - 12/16/21 08:00 AM (4 years, 10 months ago)

I confess that I've never used traditional LC. I've been using SSLC and it's worked so well that I've just stuck with it.

I've been cutting a 100mm plate into quarters and dropping each quarter into the SSLC media. Put it on a magnetic stir plate for 24 hours and let it sit for about a week. Usually, a thick layer of myc starts to form on the surface of the media. When that happens, I put it back on the stir plate to break the top layer into little pieces and use it nocc jars with it right away.

Lately, I've only been making media batches of 100-125mL. When I use a smaller amount of media like that, I just drop the wedge into the media and blend it for 24 hours and use it right after. Ive been using 25g of media per quart jar and that usually reaches full colonization in 5-7 days.

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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Doctor Mario]
#27583488 - 12/16/21 11:16 AM (4 years, 10 months ago)

With small amounts of sslc just dropping a wedge and letting it sit on the stirrer for 24 hours makes it mixed enough to finish jars in a week?

That’s pretty cool, like no blender LI.

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Doctor Mario
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27583522 - 12/16/21 11:52 AM (4 years, 10 months ago)

It's been working for me. The wedge doesn't really dissolve at that point but the stirrer shreds most of the myc off of the surface and mixes it up in the media.

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milkboy
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Registered: 05/01/21
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Doctor Mario]
#27583590 - 12/16/21 12:52 PM (4 years, 10 months ago)

I made some SSLC recently, one came out just fine, the other became a jello brick with some water around it. I shook the shit out of it and it broke up ok but is a very different consistency then the other. Same recipe for both, wtf happened? Any ideas? Did I not mix it up enough? No way I was that high to fuck up the recipe that bad, or maybe I was. The jar I shook now has a permanent layer of bubbles about an inch thick .

Id post pics but not in the Lab at the moment.

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