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Re: AGAR ENVY! (Anything and All things agar!) [Re: Lectric]
    #27377937 -

Thank you lenz

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Re: AGAR ENVY! (Anything and All things agar!) [Re: B H O]
    #27377946 -

B H O said:
I wait a whole day sometimes longer before I use my plates. I don’t always use all my prepared plates in one shot and have some sitting for a few days at a time.

Also yea you wanna cool into the receiving agar and use immediately. Sometimes I use the top stacked plate that has condensation and use that as my cooling plate.



Oh wow, alright i'll slow down next time. Just hoping i didn't jeopardize samples.

I actually started using one plate for cooling, then thought that plate could get contams from being opened/closed so often, so i switched to cooling in receiving plate.

Thanks man!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Lectric]
    #27378010 -

I hate when water pools around the wedge. Only happened a few times.


I never use all the plates at once either, I usually have 20-30 wrapped in stacks of four on deck.


--------------------
LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #27378261 -

I try to pour enough to last me several weeks but I’ve also slowed way down on my agar to grain and doing more g2g of decent cultures

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27378267 -

Went through a patch of terrible outbreaks on agar and think I finally got to the bottom of it...thanks reddit stickers.

Germing PE trinity and Pan Estero. and working with a cube variety to see if I can overwinter/naturalize it...Failed hispanica and mexicana this year and want to revisit those, but tend to get overwhelmed. Also eyeballing sempervia.

Cult, what should I germ? Semp, gal-mexs, or hisp

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rockyfungus]
    #27378280 -



Any reishi experts? It’s my first time and don’t know what I’m looking at. Mycelium looks kinda different compared to cubes. The one labeled #2 I missed the center with the transfer so I slide it into place and so I think that’s that little patch of growth.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rockyfungus]
    #27378296 -

rockyfungus said:
Went through a patch of terrible outbreaks on agar and think I finally got to the bottom of it...thanks reddit stickers.

Germing PE trinity and Pan Estero. and working with a cube variety to see if I can overwinter/naturalize it...Failed hispanica and mexicana this year and want to revisit those, but tend to get overwhelmed. Also eyeballing sempervia.

Cult, what should I germ? Semp, gal-mexs, or hisp



Both :rasta:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: B H O]
    #27378301 -

B H O said:


Any reishi experts? It’s my first time and don’t know what I’m looking at. Mycelium looks kinda different compared to cubes. The one labeled #2 I missed the center with the transfer so I slide it into place and so I think that’s that little patch of growth.



The first picture looks like it could use a transfer but the others look good to me.

I wouldn’t say I’m an expert by any means but I’ve gotten 2 bags to fruit, so I’m not totally unfamiliar.

Love this cluster


--------------------
Mehhh



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Re: AGAR ENVY! (Anything and All things agar!) [Re: Wall.E]
    #27378327 -

Thanks! That is a beautiful cluster!!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Wall.E]
    #27378335 -

Found some old plates/pictures I forgot in my basement. Photodump...





transferred a pin, got zipped down really quickly

said pin x-fered

Edited by rockyfungus (07/08/21 02:04 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27378488 -

A.k.a said:
I hate when water pools around the wedge. Only happened a few times.


I never use all the plates at once either, I usually have 20-30 wrapped in stacks of four on deck.



I like that idea of wrapping stacks, I'll try that when i buy seran wrap after para is all gone :laugh:

parafilm is incredible, but i dislike cutting individual pieces/separating. i actually can't wait till i run out...lol


D3monic said:
I try to pour enough to last me several weeks but I’ve also slowed way down on my agar to grain and doing more g2g of decent cultures



for sure man, i definitely look forward to g2g!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Lectric]
    #27378753 -

Damn I'm still getting satellites around the edges of my petris. Only ones I've used the wrapped ones are good..

:notsureif:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushboy]
    #27378789 -

how do u store ur plates after pouring mushboy?
do you wrap them all individually or put it back in its og sleeve or saran wrap on the whole stack

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Re: AGAR ENVY! (Anything and All things agar!) [Re: monkey_accident]
    #27378806 -

After pouring I wrap the stacks in cling wrap. Those are good. No issues.

It's the petris I'm inoculating/transferring too. Those are all getting contamination right at the edges..I use parafilm to wrap them. I really dont want to have to store them in bags or something that's lame as fuck:mad2:

In Sab days I left my petris unwrapped inside the sab and rarely had a problem.

Just looking thru my stacks of throw away old petris they damn near all have the same yellow sporulating mold. I'll take pics at home.

:oldman:

Edited by mushboy (07/08/21 10:39 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushboy]
    #27378808 -

I love parafilm and am very thankful for it.


--------------------
Question everything, it's very responsive.


Culture Storage

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Re: AGAR ENVY! (Anything and All things agar!) [Re: B H O]
    #27378813 -

ok. Thank you. I'm going to transfer some to some new plates. I'm going to drop a few wedges in some jars and make some syringes for a few other jars just to try everything out. No work today so I've got plenty of time to play. I made some grain jars last night. I couldn't find rye so I ended up using farro. It's bassically like a wheat berry. I think I overcooked it a bit. I can't really shake up the grain. It's basically stuck in jar/ brick form. It moves around in the jar but doesn't break up. Is that going to be a problem? should i start over on the grain? I appreciate all the help. I realize this isn't the newb section but you guys are very responsive.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Sweetdog760]
    #27378829 -

That's weird mushboy, just around the edges and only after transferring to/from them... hmmmmm
beats me I hope u figure it out thats frustrating for sure

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Re: AGAR ENVY! (Anything and All things agar!) [Re: monkey_accident]
    #27379000 -


Sexiest ms plate to date. May not be much but shes mine. 10 day old APE

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Re: AGAR ENVY! (Anything and All things agar!) [Re: monkey_accident] * 3
    #27379030 -

It puts the lotion on its skin or else it gets the contams again.

Mark the side of the plate your angle of approach was. See if it coincides with your contams. Will give you an idea if it’s falling onto plate from hand or scalpel.  Or if it’s in the 12oclock from you then good possibility it’s getting blown in. Where're you setting lid while you’re xfering?

I’m just helping you think, you’re seasoned I know none this new to you but sometimes a little mental prodding gives us the direction we need for that ahha! Moment

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27379055 -

Right on bruh. Good tips:rockon:

I've been around for awhile but flow stuff I'm a total noob so anything even remotely obvious is greatly appreciated. All my inoculated jars are looking good so considering its only(so far) on plates around the edges its gotta be something I'm doing.

If my poured wrapped stacks mold I'll really panic
:tryingnottodie:

Edited by mushboy (07/08/21 01:42 PM)

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