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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
    #27357438 -

Yeh that's what has me worried, the transfers are also quite wispy and don't give me much confidence but i'qll see what happens

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27357439 -

That's always the best move. Either way, it's a learning opportunity. Go for it and school us after.


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
    #27357442 -

I'm wondering if it's the batch of agar but ill only know once I make a new batch and change up the ingredients.
This last batch was grain water, lme and yeast, going to change it up to straight lme agar and see how the culture responds.

Edited by DERRAYLD (06/21/21 01:01 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27357444 -

Logical.


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
    #27357606 -



Very nice rhizomorphic T1 growth from the albino Louisiana but pretty faint, fluffy mycelium from the TAT I put on the same plate. It doesn't look like they're overlapping yet but the plate is clean so Im happy. The melmak plate is the same I posted yesterday, also looking good. I've noticed that my older plates turn a brownish color. I don't think this is contamination as there are no other signs of it so I'm guessing it has to do with the food coloring.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mandelbro]
    #27357610 -

mandelbro said:


Very nice rhizomorphic T1 growth from the albino Louisiana but pretty faint, fluffy mycelium from the TAT I put on the same plate. It doesn't look like they're overlapping yet but the plate is clean so Im happy. The melmak plate is the same I posted yesterday, also looking good. I've noticed that my older plates turn a brownish color. I don't think this is contamination as there are no other signs of it so I'm guessing it has to do with the food coloring.


It’s way harder to get them to cross this way


--------------------
Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Eugene Gesuale]
    #27357619 -

Oh is it? I would have thought it would be more direct than just mixing grain spawn. I am planning to do that anyway with this plate so I'll have another chance then.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mandelbro]
    #27357646 -

Iirc when you mix spawn from 2 ms cultures you have more opportunities for dilaryons to share genetic info. Giving you a more diverse flush. Someone correct me if I’m wrong.
If you mixed spores from each substrain you are more likely to get spores to germ, then the monokaryons to meet up and mate one with another from each strain


--------------------
Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Fresh Prints]
    #27357655 -

The Fresh Prints said:



Okay, enough of this AGAR PORN, with your perfectly round samples...

The Fresh Prints, D3monic, Crackatoa... you guys gotta show me your tools... do you cut and modify the SS straws with a handle or do you just use them "bareback" and risk burning your fingers?

Do tell, pics appreciated! I've got a 6 pack ready to go...

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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVandalx]
    #27357670 -

Pretty sure they're using leather hole punches.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVandalx]
    #27357679 -

I’ll be holding a contest soon once I’ve fulfilled all my other mailing obligations for one these bad boys




Along with some other goodies.


--------------------

D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27357694 -

Mines just a straw straight from the package. Still has the little silicon thing for the mouth piece.


--------------------

Crack's Tek's and Shit


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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27357794 -

D3monic said:
I’ll be holding a contest soon once I’ve fulfilled all my other mailing obligations for one these bad boys




Along with some other goodies.


Wow, I feel really jealous now... thanks for show and tell!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVandalx]
    #27357801 -

Those are pretty neat D3. I thought of making one like that with a 90 bent on the end just cause I don't have a hood and keep the lid over my petri but it's hard to justify the work when a straw works well.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Drboomer]
    #27357835 -

How do you force the segment out of the straw?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mandelbro]
    #27357844 -

Almost always it dosnt stick in the straw. It's just basically a cookie cutter then you transfer with a scalpel.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Drboomer]
    #27357853 -

Ah, I see. I'll give this a try, thanks.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mandelbro]
    #27357891 -

DERRAYLD said:
I'm not liking the satellite ring or the look of the mycelium but I can't decide if it's contamination at this stage.


many of my plates show this going on too.  Try making half / half plates one low nutrition one high nutrition and transfer out some of that clear halo only. For me personally it did't change much and a lot of the plates just grew weak myc no matter- but at least you'll be sure it's not your agar. I think its just genetics at this point because i've had problems with transparency and fuzzy myc past 2 months and it seems of all my plates like 20-30% of the bunch are always very weak looking.

mandelbro said:
Seeing strong mycelium on this T2 Melmak plate.




many of my plates have that fuzz especially growing off the transfer piece, so i take it they are all normal? i was under the impression that its white molds meshed in


--------------------

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Re: AGAR ENVY! (Anything and All things agar!) [Re: moshe1]
    #27357906 -

That fuzz is mycelium.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: moshe1]
    #27357910 -



This clone has grown out pretty much all the way today, this pic is from a few days ago. Its from an invitro pin.

This is my best looking plate so far, and also the only clone plate that i have to work with atm. I want to process it properly and not lose a potentially good culture. The current plan is to xfer out of this plate to new some new dishes and put this one in the fridge just incase things go wrong.

The fact that clones have genetic variation really confuses me for slanting them- since transfers hold smaller portions of a clones complete genetic make up, it worries me that you could easily lose your good genetics after a couple grow cycles.


mandelbro said:
That fuzz is mycelium.


Nice, thats music to my ears cause i've gotten a lot of mixed answer for fuzz. Heres a contam plate i never put any myc spores on, that white fuzz is all contam. I am convinced its what got into a lot of my plates; it makes it really hard to discern between it and tementose myc. Never changes color or form.



Edited by moshe1 (06/21/21 07:46 PM)

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Shop: Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Substrate Bags   Myyco.com APE Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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