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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa]
    #27337381 -

This the one I’m using, haven’t tried it on foods yet but does have sort of a cheesy smell. Myc been loving it.

Anthony's Premium Nutritional Yeast Flakes, 10 oz, Non Fortified, Batch Tested Gluten Free, Non GMO https://www.amazon.com/dp/B07JVLCGYX/ref=cm_sw_r_cp_api_glt_i_4PD0BNBPDV84D11FZGMS?_encoding=UTF8&psc=1

Makes agar cloudy so been using the charcoal for my yeast plates.

https://www.amazon.com/dp/B07DWCMRQ6/ref=cm_sw_r_cp_awdb_imm_0RHQ8AV68D61TYP54ZVJ?_encoding=UTF8&psc=1

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27337428 -




Just getting started so this is my first post.
Cloned Pleurotus eryngii that I bought in the local store. So far doesn't look that bad. The sample was taken on 23rd May, so it has been growing for 2 weeks already.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SterlingShroom]
    #27338081 -

I may have put too many spores on this plate.

Is it too soon to make a transfer if so any recommendations on a good area?

Looks chaotic through my eyes.


Thank you


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Atomsplit]
    #27338094 -

You could  Definitely make a transfer now

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Big_Dub]
    #27338101 -

Big_Dub said:
You could  Definitely make a transfer now



Would 6 o'clock & 8 o clock be a bad spot?

Thank$!


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.

Edited by Atomsplit (06/06/21 11:26 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Atomsplit]
    #27338105 -

make a bunch of plates from a bunch of different spots 

This will help you learn what to look for

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Big_Dub]
    #27338137 -

Big_Dub said:
make a bunch of plates from a bunch of different spots 

This will help you learn what to look for



Thank you Big Dub I'll try my best to decipher it!


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Atomsplit]
    #27338198 -

Couple of plates two hours ago.





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SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27338209 -

You got several good places for transfers. Looking good SB.


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Crack's Tek's and Shit


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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27338341 -

DERRAYLD said:
There are guys that have purchased or made biopsy tools like that with an internal rod to push the slug out.




D3monic said:
Out of several thousand transfers I’ve had them stick maybe 3-4 times. Usually if tool too hot or if your tool has a bit of wall thickness.



thank you for the replies.
i will try the suggested method first and maybe buy or make a tool later down the line for convenience. 3-4 out of thousands is not too bad. i might even have a metal straw lying around, just got to remember where.

thank you for the help

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Re: AGAR ENVY! (Anything and All things agar!) [Re: greenladel]
    #27338620 -

This was an isolated cube according to the guy that gave it to me.....
Isolated you say?



Sorry pp is not nice for photos.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27338644 -

Almost anyone who says they have an isolate is confused. Not all, but many.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: verum subsequentis]
    #27338751 -

At least the lc was clean, ill give him that.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD] * 1
    #27338962 -

Would be very very hard to determine an isolate from mycelial patterning. The pattern is not always indicative. Even an isolate will express it's self differently depending on conditions. For example the density or nutrient content of the agar media could vary from centimeter to centimeter causing a culture to appear conflicting or running growth next to tommy growth etc.

That is a very pretty culture regardless and it should prove to be be a prosperous endeavor working with it. Stoke.:super:


--------------------
Question everything, it's very responsive.


Culture Storage

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome]
    #27339053 -

Few of my plates. One got the lumpy shit at the end of pouring my plates . Growing fine right over the lumps

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Culliganman360]
    #27339300 -


couple of pretty plates, that pink buffalo and pes-Hawaii are some of the best looking germ plates I've had in awhile gonna transfer those now
the t3 brazil went to grain already, gonna g2g that soon -  and the boobs plate was a t4 of B+ that melted off my scalpel mid transfer :lol:


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helpful links
my FH build

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Re: AGAR ENVY! (Anything and All things agar!) [Re: monkey_accident]
    #27339306 -

x3 P. natalensis clones and x1 P. allenii clone. :grin:



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PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear


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Re: AGAR ENVY! (Anything and All things agar!) [Re: monkey_accident]
    #27339309 -

One of a few t1 plates that look similar to the others. Would any of you feel comfortable with throwing this to grain? Only thing I'm not sure about is the little tufts that reach up, but if looked at with the light it just looks like myc fanning out and up





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Re: AGAR ENVY! (Anything and All things agar!) [Re: GandalfTheWhite]
    #27339386 -

MELMAK T1s i havent touched yet, i think they look good?



Blue = 5G LME to 10G AGAR    Green = 7G LME and 1G Yest to 10G AGAR



more fuzzy growth i cant figure out



PE6 still showing weak thin growth


Edited by moshe1 (06/07/21 09:27 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: moshe1]
    #27339399 -

The Mak T-1's have some good places to transfer from and places I'd snag a piece from and throw to SSLC.
The fluffy ones I would let grow out some more, I have some that start like that but rhizo out by the edge of the plate for transfer's. I feel like something is riding along with your PE6, what's your PE6 germ plate look like?


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Crack's Tek's and Shit


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