sporecap said:

@xspak:
Very interesting! Did you sterilize the slide (if so, how) or just took one from a fresh pack?

Just did the poor man's version of the microscopy slide:

-Sterilize scalpel and cut a small well in a new agar dish (a bit off center)
-take a small transfer from the bacterial plate and put it in the well of the new plate
-sterilize scalpel again
-cut a large square agar wedge from the new plate and put it over the well to cover the transferred agar piece.

Will post results as soon as available.

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Here's an update on the agar sandwich method after 4 days. Growth speed was almost not affected, the myc seemed to have no problem to grow through the agar when being confined. In almost all plates it dominantly grew upwards and then emerged on top of the agar wedge, something which I did not expect. I thought it might be easier to squeeze through between the interface of the wedge and the agar plate, but it was definitely slower in that direction. The emerging agar was super thin and whispy, exactly like the translucent growth one sometimes gets from spores germinating on agar. Maybe this is a general feature of myc that actually growths through the agar? Once it stays on the surface it changes into a more rhizomorphic form?
I took transfers by scraping the top of the agar wedge with an inocculation loop, the amount of myc transferred was not detectable by eye (but I'm pretty sure there was some). Let's see what it does next.
If this proves to work well, I will implement it in my workflow: Spores to agar -> Transfer after Germination into Agar sandwich -> Transfer to normal plate.

A.k.a said:

I just realized it could be your coloring too.

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D3monic said:

It’s been found that the magenta and purple can mess with the myc as there’s red #3 which has fungicidal properties. I’m still meaning to do a side by side test on this but going back through all my pics seems to back up that data. All the plates that did weird stuff was on those colors.

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Inthepit said:

Most peeps are on Team Telephone Agar Agar, while some don't
care and others think it's not good enought! 
Anyway it's good to include what Brand yer using also.
Team Telephone FTW! 

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Inthepit said:

Most peeps are on Team Telephone Agar Agar, while some don't
care and others think it's not good enought! 
Anyway it's good to include what Brand yer using also.
Team Telephone FTW! 

---

Inthepit said:

Most peeps are on Team Telephone Agar Agar, while some don't
care and others think it's not good enought! 
Anyway it's good to include what Brand yer using also.
Team Telephone FTW! 

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