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Re: AGAR ENVY! (Anything and All things agar!) [Re: xspak]
    #26982223 -

https://imgur.com/a/yeXda5e

Is this Lion's Mane plate ready for grain? I can't see any growth or development on the surface of the place, just the "internal" growth. I'm worried about it not taking off on grains. What do you think?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: redbee]
    #26982237 -

redbee said:
No-pour day! Filled up a shoebox with these, we'll see how long that lasts me.





And here's a T1 of a TOC clone I took from a fruit that was showing some PE traits (fat knobby stem, small cap, light spore-load.)



I dropped a wedge onto grains, we'll see how it goes. The cloned fruit was on the small side, so hopefully there'll be some size variation.



:homerdrool:


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HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tripdawg420]
    #26982297 -

On todays episode of drunk people doing lab things. Pouring stacks


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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26982419 -

What’s that tool you’re using to cut the sleeve with?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Yeetusdeetus]
    #26982494 -

It came with a pack of sleeves. Sort of like a letter opener.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nogoodnamesleft]
    #26982512 -

nogoodnamesleft said:
Big shoutout to the Shroomery and Myers Mushrooms for teaching me how to do agar.

Got my flowhood unboxed today and poured plates same day! Boom!





that is what we call one bad ass motha fucking flow hood.

like god damn :boobs:



also nice even pours :thumbup:


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"You didn’t come into this world. You came out of it, like a wave from the ocean. You are no stranger here."


Edited by Rafiikii (10/13/20 12:33 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rafiikii]
    #26982578 -

all my xfers are very light these days.. running equal part agar to lme. this is like a 3rd of 4th transfer.

any tips?



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"You didn’t come into this world. You came out of it, like a wave from the ocean. You are no stranger here."


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rafiikii]
    #26982602 -

Blue magnolia pin from a MS plate


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rafiikii]
    #26982605 -

What the heck is up with agar?

This stuff is addicting.

It’s also seeming to be an excellent way to stretch out spores !

I have 40 PFC and 40 RW plates going now and I used about 2mL of each total for all 80 plates

I’m truly amazed.

Stuck some inner tissue from a Golden Teacher cluster on a plate and also swiped some spores from prints I made off the first fruits I’ve harvested in 15 or so years..

Today’s plates were on the seemingly favorite of the Shroomery 2% agar and LME , i supplemented with activated charcoal again. I really think there’s something to germinating spores with the carbon on plates. A porous surface provides a surface for the mycelium to attach to.

I have 25% of the plates on non carbon supplemented LMEA  to compare germination times
—

Also want to throw out for other people just starting in this or even in it for a while ,  buy or build the damn flow hood. Yes you CAN get by other ways, but just don’t do that to yourself.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nogoodnamesleft]
    #26982632 -

Interested to see the effect of the charcoal on germination, I've had two spore syringes I couldn't germ. No more than 6 months old from a reputable vendor. Gonna try softer plates but the charcoal plates also just look dope too :cool:


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:chems:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26982730 -

Cool video D3, almost like being there.
Just curious, did you have a sous vide in that pot of water?
And it looked like you pour around 139F? Not sure it was hard to catch it.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Inthepit]
    #26982766 -

No sous vide, just heat the water to 145. As you see it only takes me less than 10 min from prep to last pour to do a 40 stack. The media bottles are loaded right from pc to water bath. Water bath helps cool them down and maintain a pourable temp. I pour as soon as they are cool enough to handle usually 130’s as I hate when it starts to gel up on me. Since i leave them in front of the hood a few hours most the condensation goes away. I really wanted to capture the resleeve but just picture stacking plates in stacks of 20 and sliding the sleeve they came in back over them.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26982840 -

It's so hard to resleeve in a SAB. I want a flowhood so bad by my space is limited :/ great videos, D3!


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:computertrip:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: RhYzo]
    #26982864 -





Mushroom myc or other? also nogoodnamesleft how much was that flowhood, got a link?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Robin-Forepaper]
    #26982870 -

First plate I’d transfer a tiny tip from about 4:30-5 oclock. Second not sure, pics aren’t really that clear.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26982969 -

noggodnames has a Terra Universal horizontal valueline hood. Model 1688-43A.

my quote from Andre@terrauniversal.com was $2283. Do me a karma solid and buy it from that guy. Offer him $2100 and see what he says. I would have but I wound up going with a SMOKING scratch and dent deal from Air Science, but that Andre at Terra was super cool and responsive and he's working on commish. Would be cool if somebody from the community gave him a sale. They ship out of Cali and usually have that model ready to ship in 1-3 days.

after weeks of research, notwithstanding my Air Science score, if you want a hood NOW and don't want to build one out or wait to have one built and like the idea of an enclosed, mobile-ish hood, the TU seems the way to go.

Didn't mean to hijack the question, nogoods.  But I wanted to take the opportunity to refer a sale to my guy at Terra who I left in the lurch after he worked for it, returned comms afterhours, etc.

Edited by nmd_myco (10/13/20 11:11 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nmd_myco]
    #26983057 -





@ D3 thanks man, you're right those pics did suck / were unclear lol, new at the photography thing.. hopefully this is better. pic 1 is a better depiction of how my transfers seem to turn out even after transferring from an agar pin (like pic2). the pin myc looks fine to take transfers.. so the question is basically am i actually that trash with sterile tek? and am i trying to outrun mold or is that just heavily bacterial (pic1)? also thank you @ nmd_myco if that's what i go with i'll definitely shoot dude an email!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nmd_myco]
    #26983062 -

nmd_myco said:
noggodnames has a Terra Universal horizontal valueline hood. Model 1688-43A.

my quote from Andre@terrauniversal.com was $2283. Do me a karma solid and buy it from that guy. Offer him $2100 and see what he says. I would have but I wound up going with a SMOKING scratch and dent deal from Air Science, but that Andre at Terra was super cool and responsive and he's working on commish. Would be cool if somebody from the community gave him a sale. They ship out of Cali and usually have that model ready to ship in 1-3 days.

after weeks of research, notwithstanding my Air Science score, if you want a hood NOW and don't want to build one out or wait to have one built and like the idea of an enclosed, mobile-ish hood, the TU seems the way to go.

Didn't mean to hijack the question, nogoods.  But I wanted to take the opportunity to refer a sale to my guy at Terra who I left in the lurch after he worked for it, returned comms afterhours, etc.



Thanks for taking point there. A much more concise and helpful answer than my one liner would have been!!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nogoodnamesleft]
    #26983120 -

nogoodnamesleft said:
Thanks for taking point there. A much more concise and helpful answer than my one liner would have been!!



wakeup dabs and words by the bag.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nmd_myco]
    #26983305 -

I know from reading here that it's not advisable to take transfers from overgrown plates because of potential edge contams. Is it frowned upon to take transfers from closer to the center of the culture to avoid that / does that even work?

I want to have some backup plates ready in case my grain gets fucked and I was wondering if I could use some of my overgrown first/second transfer plates.

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Shop: Original Sensible Seeds USA West Coast Strains   North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy   Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Liquid Cultures


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