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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: SpunkyMonkey88]
    #26412189 -

lol here’s some for sure bacteria I had.

Interestingly it seems like leaving it in the cold for a week let the myc kill it and take over everything.
This plate looks awesome now from the top, from the back you can see the old bacteria river.


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LAGM2020

Edited by A.k.a (01/02/20 09:36 AM)

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: A.k.a]
    #26412222 -

A.k.a said:
lol here’s some for sure bacteria I had.

Interestingly it seems like leaving it in the cold for a week let the myc kill it and take over everything.
This plate looks awesome now from the top, from the back you can see the old bacteria river.



Eww dude that looks like jizz lol

Well mine definitely doesn't look like that but its definitely some kind of clearish cloudy contam that the stopped the mycelium dead in its tracks...

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: SpunkyMonkey88]
    #26412242 -

Lol yeah that plate was shocking.

I think it has to be bacteria causing the clear/thin looking growth. I had a couple plates like that where they get the Pac-Man mouth.


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LAGM2020

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: A.k.a]
    #26412393 -

Would there be any issue with me freezing plates or just the agar (after PC) for storage?


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CBM's 2020 Psilocybe Aztecorum Grow (any advice, info, links, guidance welcome here) Journal

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: Colorblind-Myc]
    #26412552 -

I’m pretty sure agar stays good for a long time, but you could always throw a plate in the freezer for a few hours and see.


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LAGM2020

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: A.k.a]
    #26412591 -

Put them in the fridge, not the freezer. The water inside the agar will freeze, but the rest of the material won't freeze, and when it warms back up, it's not quite the same. There is a high chance of condensation forming, and when that falls back onto the agar surface, it'll try to suck it all back up, making it really soupy.

Edited by MLPismyOPSEC (01/02/20 01:59 PM)

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: MLPismyOPSEC]
    #26412626 -

Really? I've been freezing mine for a long time never noticed any problems.
It does seperate and then has to be heated back up to remix but I never got any soupy agar. The difference if any hasn't been noticeable for me.
I definitely wouldn't freeze plates though.


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Trade List
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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: gizmo1]
    #26412658 -

gizmo1 said:
Really? I've been freezing mine for a long time never noticed any problems.
It does seperate and then has to be heated back up to remix but I never got any soupy agar. The difference if any hasn't been noticeable for me.
I definitely wouldn't freeze plates though.


Ya I think he's talking about freezing plates...

Freezing and reheating agar shouldnt matter though...

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: SpunkyMonkey88]
    #26412691 -

Caps - Seems totally plausible that (depending on the culture) one cud continue in the fashion you do for some time without much of an issue, but the act of narrowing genetics in that way is still very real.

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: SpunkyMonkey88]
    #26412721 -

SpunkyMonkey88 said:
gizmo1 said:
Really? I've been freezing mine for a long time never noticed any problems.
It does seperate and then has to be heated back up to remix but I never got any soupy agar. The difference if any hasn't been noticeable for me.
I definitely wouldn't freeze plates though.


Ya I think he's talking about freezing plates...

Freezing and reheating agar shouldnt matter though...


So plates probably not. Was planning on making a few different agar mixes, save the excess


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CBM's 2020 Psilocybe Aztecorum Grow (any advice, info, links, guidance welcome here) Journal

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: Colorblind-Myc]
    #26412760 -

Colorblind-Myc said:
SpunkyMonkey88 said:
gizmo1 said:
Really? I've been freezing mine for a long time never noticed any problems.
It does seperate and then has to be heated back up to remix but I never got any soupy agar. The difference if any hasn't been noticeable for me.
I definitely wouldn't freeze plates though.


Ya I think he's talking about freezing plates...

Freezing and reheating agar shouldnt matter though...


So plates probably not. Was planning on making a few different agar mixes, save the excess


Ya but if it's in sealed media bottle itll keep forever in the fridge too... or probably even in a cupboard as long as u didnt contaminate it..

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: SpunkyMonkey88]
    #26414042 -

Hey guys. Just looking for some feedback on these plates.
Dont mind the mold spots :lol:



The PM#1 and AC#2 look pretty good to me but I am un sure about the LGT plates... LGT#3 looks like it might be ok?


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Tek's I use

LAGM2020

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: staytrippy420] * 2
    #26414086 -

Its obvious where to transfer from from the good plates but id transfer to a new plate first sign of growth if they have mold spores now.

The LG plates just need more transfers.  I take it this is from spores or a print?  Sometimes if I cant get a culture clean I try to get a colonised jar from a good section and then clone the first mushrooms grown in a shoebox  OR leave a suspect plate till it pins and transfer that to anew plate.

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: staytrippy420] * 1
    #26414137 -

All LGT's are probably 2-3 transfers away from looking really good.
LGT1 - 4:00-5:00
LGT2 - 7:00-9:00 on the smaller cluster
LGT3 - either 12:00-2:00 or 4:00-5:00.

PM+AC plates are 2-3 transfers away from looking hella dope.
PM1 - that 12:00-1:00 is hot, 11:00-12:00 would be really uniform on the next plate, but literally anywhere from 7:00-2:00 is good
AC1 - give some more time, but 8:00-9:00 or 4:00-5:00 look the most promising
AC2 - anywhere in that 8:00-11:30 area. looks sooo gooooood

LGT2, PM1, and AC2 look like they have some bacteria (hence the thin/void-looking areas). LGT3 looks good to go to grain personally, but i'd give it time.

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: MLPismyOPSEC]
    #26414155 -

Right on. Thanks guys. :rockon:
They are all from prints.

I'll prepare the sab and take some transfers away from the moldy plates.
And I'll leave LGT#3 and AC#1 for a few more days.


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Tek's I use

LAGM2020

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: staytrippy420] * 1
    #26414202 -

staytrippy420 said:
Right on. Thanks guys. :rockon:
They are all from prints.

I'll prepare the sab and take some transfers away from the moldy plates.
And I'll leave LGT#3 and AC#1 for a few more days.


Move slowly with these and as said have a 2 plate clean up. Transfer and first slightest growth transfer again.  U can alway take 2 plates each culture and just leave one to see what happens.

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: Edmunter]
    #26414310 -

Are these little circles what it looks like when a single spore or spot of myc grows by itself??


Took me a few tries to get the chunk off the blade but it looks like everyplace that I touched grew.

I’m also looking for input on these plates, I’m definitely making progress.



What’s the deal with the dark areas surrounding the transfer chunk?? It seems to be culture dependent, all my maz and gt have it but not PE or Burma.


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LAGM2020

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: A.k.a] * 1
    #26414477 -

A.k.a said:
Are these little circles what it looks like when a single spore or spot of myc grows by itself??


Short answer; not necessarily, and probably no. with just how tiny those microscopic spores are, you would be hard pressed to actually select just one, even with a microscope and correct tools. Agar/transfers/spore prints would be essentially impossible to get one spore. Plus i believe you need two spores to start any growth at all, but that's being a little nitpicky.

A.k.a said:
Took me a few tries to get the chunk off the blade but it looks like everyplace that I touched grew.


:laugh2: it happens brah! I had a wedge that rolled over when i was wrapping the plate, and there were two very distinct origins.

From starting agar a month ago to this, you are kicking ass man! The pic of three plates all look good, i wouldn't be afraid to put those on grain. About your last question, I believe it's just thin mycelium, i have a ton of plates that look just like that.

Edited by MLPismyOPSEC (01/03/20 02:27 PM)

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: MLPismyOPSEC]
    #26414489 -

Slow mexicana growth? These ps mexicana plates are now 2 weeks old and seem very slow to grow.

Temperatures here are great for this species, agar is MEA 10:10:500ml

Plates seem clean, just slow!

Am considering transferring to lower nute plates.

Any thoughts?


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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: bongoman]
    #26414516 -

Yeah go down to 8g nutes per 500ml

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