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Re: super wacky semi projectt in my slums log [Re: meaculpaUIO]
    #26270029 -

Guess what Q tips suck ass for tall jars.

Comments:

- Shaky, fiddly in the beginning.
- A few times I hold my hands over shit, getting more conscious of that.
- Obviously the short length of the swabs betrayed me this round, but hey at least Im not using them for anything else than cleaning up the spore print.
- Torched my finger
- Its not a good idea to cool this inoculation loop in agar, because it will
actually bring agar back with it to the spore print
- having to clean up agar spill on your spore print obviously isnt a great idea
- double dipping the last jar

Edited by meaculpaUIO (10/22/19 03:35 PM)

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Re: super wacky semi projectt in my slums log [Re: meaculpaUIO]
    #26272314 -

You are improving and it sounds like you are re-watching your videos for where you messed up :thumbup:  Second video - don't put your bare wrist over the spore print and scrap the whole Q-tip thing completely.  In my opinion they don't have any place with what you are trying to do. 

It doesn't take very many spores to inoculate a plate.  If you are concerned about contaminating your print with agar from your loop, one option is to tear the foil and the print into smaller sections (while inside the SAB).  This way you can store away the larger print and just work off of the smaller piece you tore off.

P.s. Sorry for calling you a troll, but when I watched the video with the Q-tips and the jar of water I genuinely thought you were screwing with us.  Still looking forward to seeing you succeed.


--------------------

Last Project: LAGM: Stones From Scratch in 2020


Edited by DcevLearn (10/23/19 05:40 PM)

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Re: super wacky semi projectt in my slums log [Re: DcevLearn]
    #26272381 -

Thank you a lot, that means something to me. Made some transfers today, having a ball with buying these glass jars with metal lids at the local convenience store, tomato paste 0.70$, taco sauce even cheaper 0.55.

There is now a mason jar without any visible contams, and with seemingly strong mycelium.

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Re: super wacky semi projectt in my slums log [Re: meaculpaUIO]
    #26272391 -



Is this getting ready for transfers to other agar or jars, team Shroomery?

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Re: super wacky semi projectt in my slums log [Re: meaculpaUIO]
    #26272489 -

If you got white growth, and it bruises blue, you can be sure right?

The reason I am asking this, is because when I worked with Agar
transfer today in the SAB, I noticed the agar plate mycelium
that I was messing with, started to bruise several places.



RogerRabbit said:
A colonizing cake won't bruise blue.  It's very easy to tell mold from bruising.  Bruising is the color of the mycelium, while mold spores will look like a layer of colored sand on the surface of the mycelium.
RR


Does this mean mycelium on agar cant bruise blue?

Edited by meaculpaUIO (10/23/19 06:54 PM)

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Re: super wacky semi projectt in my slums log [Re: meaculpaUIO]
    #26272658 -

It depends on the stage of growth. If the mycelium is past its vegetative/colonization stage, it can bruise blue, inevitable of what it has grown on. RR was speaking specifically of colonizing.

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Re: super wacky semi projectt in my slums log [Re: footpath]
    #26273700 -

Yes, myc can bruise blue when transferring.  I often swipe the whole surface of a plate just to watch it turn after my transfers are finished.  It can take anywhere from a few seconds to 15 minutes, in my experience


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Ὡς οὖν εἴπεν αὐτοῖς ὅτι Ἐγώ εἰμι, ἀπῆλθον εἰς τὰ ὀπίσω, καὶ ἔπεσον χαμαί.

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Re: super wacky semi projectt in my slums log [Re: naturalistic123]
    #26273717 -

interesting, I've never tried that. I'll have to give it a go.


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: super wacky semi projectt in my slums log [Re: verum subsequentis]
    #26273735 -

You have that big damn wall of petris and have never just smudged your finger across one you are gonna throw out!?

I feel like it's something everyone should do.  It also gives you that fresh mush smell!


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Ὡς οὖν εἴπεν αὐτοῖς ὅτι Ἐγώ εἰμι, ἀπῆλθον εἰς τὰ ὀπίσω, καὶ ἔπεσον χαμαί.

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Re: super wacky semi projectt in my slums log [Re: naturalistic123]
    #26273821 -




There ya go.  This one took a couple seconds.


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Ὡς οὖν εἴπεν αὐτοῖς ὅτι Ἐγώ εἰμι, ἀπῆλθον εἰς τὰ ὀπίσω, καὶ ἔπεσον χαμαί.

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Re: super wacky semi projectt in my slums log [Re: naturalistic123]
    #26273852 -

Hell yeah that means I have real mycelium :thumbup:

Edited by meaculpaUIO (10/24/19 11:12 AM)

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Re: super wacky semi projectt in my slums log [Re: meaculpaUIO]
    #26273856 -

you may, but then again, correlation does NOT equal causation, as any good scientist knows.


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: super wacky semi projectt in my slums log [Re: verum subsequentis]
    #26273879 -

And you might have other stuff in there too.  Transfer until youre sure there's only myc there


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Ὡς οὖν εἴπεν αὐτοῖς ὅτι Ἐγώ εἰμι, ἀπῆλθον εἰς τὰ ὀπίσω, καὶ ἔπεσον χαμαί.

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Re: super wacky semi projectt in my slums log [Re: naturalistic123]
    #26274462 -

Smoked 1/4 of a joint
Made 8 transfers using a steel wire 
Since the pore popper is missin. Made sure to give
it a good and thorough burning with my bic lighter.
Wearing face mask and gloves, but no ISO sanitizing,
this is because the burning of all the hair on my forearms
earlier today.

Im getting a camera for better pictures so we can have
more informed discussions on the growth I'm seeing in
my agar jars - ETA on this is next sunday.

Im experimenting with being generous with the amount of
colonized agar I transfer to new agar jars or to PF tek
jars. Everything I have read makes me believe that its
advantageous to the impatient and to the beginner
to use as much spawn as possible. The reasons
for this is contaminations, which the mycelium
receives a really big head start on.

Im also feeling more comfortable with my 6 L PC
because Im seeing that control jars or PCed content
stays not visibly contaminated as long as I dont
open the lids on any of my stuff.

Smoked the rest of the joint.
This is from my reading:

Dawgy Bag it!
I have aquired a few bags for this kind of use.
Are you guys against me attempting this kind of tek
as a side project?


Edited by meaculpaUIO (10/24/19 06:17 PM)

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Re: super wacky semi projectt in my slums log [Re: meaculpaUIO]
    #26275902 -

Many agar jars now exhibit what I believe to be clean, white, puffy and uncontested mycelium. There is even a PF tek jar style jar, that is about 10% into colonization. Things are looking up and I'm feeling better about the project. 

Today's task is to PC 3 jars of coir only, which I plan to make agar piece transfers into when new agar jars are colonized.

I left the coir in a bucket with water for 1H, and now Im PCing it for 60 minutes. The lids will be tightened when the PC is done, and the jars moved out of the PC to cool, so I can make some more agar jars.

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Re: super wacky semi projectt in my slums log [Re: meaculpaUIO]
    #26275907 -

DO NOT PUT AGAR INTO JARS OF ONLY COIR


EDIT -You must transfer agar into a nutritious media, which coir is not.  Grains are my choice, however you can do a multitude of things, but not coir.  Wild bird seed is cheapest for me and you can find many teks on many different things to use as a nutritious media with which to transfer your agar to.

After these have colonized, you can transfer these to coir.


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Ὡς οὖν εἴπεν αὐτοῖς ὅτι Ἐγώ εἰμι, ἀπῆλθον εἰς τὰ ὀπίσω, καὶ ἔπεσον χαμαί.

Edited by naturalistic123 (10/25/19 10:19 AM)

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Re: super wacky semi projectt in my slums log [Re: naturalistic123]
    #26275941 -

WWH*

What would happen? Would it colonize?

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Re: super wacky semi projectt in my slums log [Re: meaculpaUIO]
    #26275944 -

No.  It might branch out looking for nutrients, but there is nothing more for it to eat.  On agar, it eats whatever nutrient you have in the agar.  for me its BRF.  There is no nutrients in coir (aside from perhaps microscopic levels that are negligible and not enough for myc to live on)


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Ὡς οὖν εἴπεν αὐτοῖς ὅτι Ἐγώ εἰμι, ἀπῆλθον εἰς τὰ ὀπίσω, καὶ ἔπεσον χαμαί.

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Re: super wacky semi projectt in my slums log [Re: naturalistic123]
    #26275953 -

Coir is semi-nutritious.

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Re: super wacky semi projectt in my slums log [Re: naturalistic123]
    #26275959 -

Well good luck colonizing a jar of it with an agar wedge


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Ὡς οὖν εἴπεν αὐτοῖς ὅτι Ἐγώ εἰμι, ἀπῆλθον εἰς τὰ ὀπίσω, καὶ ἔπεσον χαμαί.

Edited by naturalistic123 (10/25/19 10:51 AM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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