Fun-G said:

You failed to explain to the people interested how a pf cake can be inoculated without a spore syringe.

And then question the use of a control jar used to test a syringe...same damn thing as testing on a petri but apparently even though it's fine for testing its ineffective? That's the definition of a oxymoron attitude.

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Fun-G said:

Contaminates dont hide, they germinate. 

If you get trich or other wise later on after a recovering transfer has already well recovered, around the 7-14 day mark it's been introduced during handling of the jar, whether that be a shaking of the grains or just over exposure to drafts.

And lc's are even more high risk then a syringe to grains, it a impossible to spot mold in a otherwise healthy looking lc unless the LC is tested a 2nd time using agar or a control jar.

If your serious about growing mushrooms prepping grain jars and using one as a control jar while the rest wait shouldn't be a huge issue, they'll remain sterile up until the seal is cracked then evaporation and gas exchange occur so the count down begins.

I dabbled in agar for cleaning up a pe swab someone sent me but that was when the source knew they were possible contaminated....and the swab was clean....only time I bothered.
And a true isolate that's used for testing bio efficiency of substrate medians would come down to two spores.... I dont have the equipment to do that, rr and workman were the only two I knew of that were that dedicated.

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Fun-G said:

If you had read the post you would have seen where I said...

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Fun-G said:

When people get into this hobby they almost always start at pf cakes, then they order a spore syringe from a sponsor. 10 out of 10 times reputable sponsors will ship clean syringes, they monitor their spore sources for contamination in a laboratory grade environment...do you think anyone would recommend a company selling inferior products?

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Fun-G said:

10 out of 10 times reputable sponsors will ship clean syringes, they monitor their spore sources for contamination in a laboratory grade environment...

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I literally can't even