Fun-G said:

A hot water bath 99% of the time will suffice  for pasteurization so long as the bulk materials dont float to the surface and stay fully submerged.

What did you use for bulk? It looks like a straw based substrate?

I wouldn't say all hope is lost, they still look ok enough to bury outside as suggested.
Here's a pic of a recent tub I did op, it defiantly had a small bacteria contaminate present in it but went on to produce 7.5oz dry in 3flushes, you still might yield on yours.

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ChardRich said:

Fun-G said:

A hot water bath 99% of the time will suffice  for pasteurization so long as the bulk materials dont float to the surface and stay fully submerged.

What did you use for bulk? It looks like a straw based substrate?

I wouldn't say all hope is lost, they still look ok enough to bury outside as suggested.
Here's a pic of a recent tub I did op, it defiantly had a small bacteria contaminate present in it but went on to produce 7.5oz dry in 3flushes, you still might yield on yours.

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You give garbage advice and tell bs stories.

OP, forget that tub, fix your spawn prep to eliminate bacteria and don't add shit to your coir.

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Fun-G said:

Do you have holes for fA.e and if so how many and roughly how big?

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Fun-G said:

.. hey now, let's tell the experienced grower to shut up while we go read more tutorials and think we cultivate mushrooms but probably dont even know how to make a clean syringe.....which is common place on here

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badsponge said:

Fun-G said:

.. hey now, let's tell the experienced grower to shut up while we go read more tutorials and think we cultivate mushrooms but probably dont even know how to make a clean syringe.....which is common place on here

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Yeah, but then you use those syringes to 'noc grain and then wonder why it's contam'ed, so...that destroys any credibility for me.

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Derailing ops thread but I have to comment.

If you had read the post you would have seen where I said I had expected to lose some jars due to my pressure cooker not sealing, so the psi hovers between 14-14.5psi when grain requires 15psi to force the heat to the core of the jars, the only reason I'm having success with 11 of 13 is because I redistributed the grains and pc'd again or else all the rye jars would have wet spot which is actually a archane bacteria found mostly in grain products.

Mushroom caps do not grow with that type of bacteria, mold spores are the only airborne contaminate that could contaminate a print but that's not the problem (nor has it ever been a issue) unless someone leaves there prints sitting out in the open in a draft then there is little chance of mold spores landing on then... my problem wasnt a dirty syringe since bacteria replicates itself very quickly, if it was bacteria introduced by a dirty syringe then all 23L of popcorn/rye would be contaminated as I stated in that thread and if it was a mold contaminate then it would be the same thing.

If all spore syringes were dirty then pf cakea would be useless, you cant innoc a cake with a agar wedge and that would be my point...dont argue or you'll group yourself into the misguided.

This is RR's advice being deformed to a unhealthy level of paranoia. He was the one that said to treat all syringes as if they were dirty...he never said all syringes were dirty but he wanted everyone to ensure success by making transfers on agar "just in case" the syringe was dirty.

Again, read what I said about pf cakes...

Another method of testing a syringe was by using a control jar... does no one use a control jar that they monitor for the days leading up to germination? Bacteria and mold will germinate side by side with cubensis spores....you dont need agar to test. No one ever brings up the control jar testing

Another thing relevant to ops post is that overlay is hardened mycelium that turns yellow...bacteria bubbles and festers when its dominate over mycelium. OP only has yellowing of the mycelium, if the tubs do not have a odor of sweet/foul then it is defiantly just a overlay issue.... I need to start collecting pictures of bacterial tubs so I have something to un fuck some peoples heads with.

Every time I post a tub of mine i get the response "bacteria" even though its healthy and fruit fine, ms genetics will give up some crazy mycelium sometimes and that gets confused for a bubbling bacteria contaminate which like I said would produce a odor, if op fans and mists and it smells like fresh mushrooms or earthy then all my money is on a overlay issue which I cant tell where to pinpoint the problem since he/she claims to mist and fan regularly.... stress and to high of a humidity would cause it... I never mist my tubs all the humidity comes from water evaporation from the substrate... invitro fruiting in bags is the same deal.

My apologies to you OP, I dont like being questioned by people that cant make a clean syringe or have zero pics of their own grows. In experience speaks for itself especially when most accounts follow that myth that all prints are contaminated... and not "to treat all prints like they are contaminated" two different definitions that have been misinterpreted.

Hopefully you try a deep scratch, if it recovers then the mycelium is healthy, if it falls to contamination then come on here and tell me that I was wrong but I'll rest assure that I've seen tubs like yours on my end before...over misting was the culprit.

Fun-G said:

You failed to explain to the people interested how a pf cake can be inoculated without a spore syringe.

And then question the use of a control jar used to test a syringe...same damn thing as testing on a petri but apparently even though it's fine for testing its ineffective? That's the definition of a oxymoron attitude.

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Fun-G said:

Contaminates dont hide, they germinate. 

If you get trich or other wise later on after a recovering transfer has already well recovered, around the 7-14 day mark it's been introduced during handling of the jar, whether that be a shaking of the grains or just over exposure to drafts.

And lc's are even more high risk then a syringe to grains, it a impossible to spot mold in a otherwise healthy looking lc unless the LC is tested a 2nd time using agar or a control jar.

If your serious about growing mushrooms prepping grain jars and using one as a control jar while the rest wait shouldn't be a huge issue, they'll remain sterile up until the seal is cracked then evaporation and gas exchange occur so the count down begins.

I dabbled in agar for cleaning up a pe swab someone sent me but that was when the source knew they were possible contaminated....and the swab was clean....only time I bothered.
And a true isolate that's used for testing bio efficiency of substrate medians would come down to two spores.... I dont have the equipment to do that, rr and workman were the only two I knew of that were that dedicated.

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Fun-G said:

If you had read the post you would have seen where I said...

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Fun-G said:

When people get into this hobby they almost always start at pf cakes, then they order a spore syringe from a sponsor. 10 out of 10 times reputable sponsors will ship clean syringes, they monitor their spore sources for contamination in a laboratory grade environment...do you think anyone would recommend a company selling inferior products?

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Fun-G said:

10 out of 10 times reputable sponsors will ship clean syringes, they monitor their spore sources for contamination in a laboratory grade environment...

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I literally can't even

Fun-G said:

I had a dehydrator.....

Until a police swat team took a battering ram to my door and ordered me to surrender or die. The landlord/mob boss/whatever went into either pissed off rage or panick mode and ordered all contents thrown out....clothes,guitar,dentures,incriminating evidence etcetc

Oh but the good news is I didnt get shot dead and they missed the mushroom patch in full bloom out back, good thing they were there on unrelated matters.

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jcm4620 said:

lol here we go back to scratch it with a fork jesus christ man. you shoulda  used your 3 month vacation to fucking study cultivation.

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jcm4620 said:

if u were a tc ever ud never have spouted this laughable shit

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