cronicr said:

10kVisions said:

thanks for all the continued opinions and help everyone.  i have made new plates using ALL syinges i have (6 total).
from each syringe i did:
1 drop plate
1 Z spread plate

then i also have to agar plates that i didn't knock
1 remained sealed with poly fill
1 i opened inside the SAB for 3 seconds and then closed it again
i figured i would do this as a control for the agar.

i'll keep evyeone posted on the process here.

on a side note, i have ordered new syringes from 2 new vendors and will see how they look when get them. (everyone was out of spore prints)

thanks again
10k

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Nobody is out of prints lol I have looked at most major vendors

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micelio said:

Just a suggestion 10k..

Order yourself some synthetic jar filter disc. cut them down to the size of a quarter. I think you should get a dozen out of every jar disc.
Silicon them to your lids. You won't have to mess with poly fill or Micro tape. They'll last for years without touching them. Use clear silicon..

They don't cost much and you get allot out of one disc..
Poly fill can wick bacteria into your jar when wet or damp..

filter disc can be use for your grain jars and agar dish..

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10kVisions said:

from each syringe i did:
1 drop plate
1 Z spread plate

then i also have to agar plates that i didn't knock
1 remained sealed with poly fill
1 i opened inside the SAB for 3 seconds and then closed it again
i figured i would do this as a control for the agar.

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I think you'll find your culpret with this methodology. Good work on isolating your issues.

Also, can I recommend looking at the market place for prints? You could even likely get one free with a giveaway of some kind. I've ordered from a shop once since I joined the forums here because the community is amazing Hell, normally I'd offer to send one out to you, but I did a giveaway last month that cleaned me out of prints complete.

hexkrak said:

All of those agar plates look completely unsalvageable to me.

Yes... people around here love to argue. Take what you will from it and do what you can not to take it personally. Everyone is arguing from their own point of experience, what worked for them, but as you can see from your own experience, the same tek might not always work for everyone. Do your best to forget what you know worked before, because obviously something is going wrong here, and until you can isolate it... nothing you're doing works.

Unless you're licking the agar plates before inoculating them, my best guess is that you have contaminated syringes. If not, then something in your inoculation process must be to blame (Innoc loop, dirty gloves, bad SAB tek like irratic movements or hovering over open containers, etc).

Along with getting some prints/changing your spore source, you might try deep cleaning your space, then going heavy with the lysol over everything. Some people have reported that this helped them a lot, but it's not a cure-all.

Also, bacteria seems to spread much more slowly over BRF pucks, so you might try those over agar and see if you can get a clean transfer from there when the myc outruns the contams.

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ShaperDreaming said:

10kVisions said:

from each syringe i did:
1 drop plate
1 Z spread plate

then i also have to agar plates that i didn't knock
1 remained sealed with poly fill
1 i opened inside the SAB for 3 seconds and then closed it again
i figured i would do this as a control for the agar.

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I think you'll find your culpret with this methodology. Good work on isolating your issues.

Also, can I recommend looking at the market place for prints? You could even likely get one free with a giveaway of some kind. I've ordered from a shop once since I joined the forums here because the community is amazing Hell, normally I'd offer to send one out to you, but I did a giveaway last month that cleaned me out of prints complete.

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Mad Season said:

For reals tho.. the people saying to review sterile tek.. the dude has the same type of bacteria in literally every plate. If this was sterile tek, he'd have a whole array of contams, not the same contam in tons of plates.. This is clearly an innoculant problem. Notice the people saying to get a proper print are all people with experience. If you don't want this shit to happen again, please get a print. Unlike a syringe that shakes everything up together, a print won't have things mixed around, and you can actually get clean spores swiped on a plate.

In the meantime, let's see how multiple syringes with control dishes work. This will once and for all lay this shit to rest, and you can finally have the first part down.. clean innoculant.

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JHOVA said:

hey make yourself a good inoculation loop from vape coil via bods tek.

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Mad Season said:

the dude has the same type of bacteria in literally every plate. If this was sterile tek, he'd have a whole array of contams, not the same contam in tons of plates.. This is clearly an innoculant problem.

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Professor X said:

JHOVA said:

hey make yourself a good inoculation loop from vape coil via bods tek.

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I made mine out of dab tools lol. Drilled a 1/16th hole in the round end. I don't even use them with syringes anymore, I just push a drop to the end then streak, I usually don't even leave the water behind.

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Ya'll, I always see people saying do this or that. Seriously, an inoculation loop from amazon with 50 replacement heads is only $10. That's $0.20 per session if you don't reuse the heads. Makes no sense to not just buy them to me

ShaperDreaming said:

Mad Season said:

the dude has the same type of bacteria in literally every plate. If this was sterile tek, he'd have a whole array of contams, not the same contam in tons of plates.. This is clearly an innoculant problem.

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100% agree with this. Also, it'll be good to know for the OP's peace of mind that it's not the technique when those test trays turn up good work.

Professor X said:

JHOVA said:

hey make yourself a good inoculation loop from vape coil via bods tek.

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I made mine out of dab tools lol. Drilled a 1/16th hole in the round end. I don't even use them with syringes anymore, I just push a drop to the end then streak, I usually don't even leave the water behind.

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Ya'll, I always see people saying do this or that. Seriously, an inoculation loop from amazon with 50 replacement heads is only $10. That's $0.20 per session if you don't reuse the heads. Makes no sense to not just buy them to me

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I was going to stay out of It, but I couldn't resist.
I tell you how fast and easy it is to make one,
A Twist tie on a loaf of bread or use in produce like celery, have a wire that runs down the center. Peal the wire off the paper/plastic and wrap the wire around a pencil to take the shape of a loop.
Fasten the loop to what ever you want for a small handle.
Remember It probably be in your hands for less than a minute.. No need to make a big deal out of It.. I been making my own for a few years now,and they work just find..
It's the same dia. and alloy as one you would buy..

micelio said:

A Twist tie on a loaf of bread

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... I can't remember the last time I saw a twist tie on one of these They're all those shitty plastic clips these days. (Also I'm fucking weird and make my own bread, so I don't pay a lot of attention to that stuff)

But! That's kinda a great idea.

Anyways, whatever ya'll want. I've only gone through 3 inoculation loops in the past 6 months since I got it (I replace when they melt... or just kinda fail when flame sterlizing. Melt isn't a good enough word, maybe pop?).

Fine fine fine fine. Ya'll make them at home. Normally I don't pony up cash for anything, but these were so cheap Seemed silly to go to a vape store, get an extra handle, twist tie that shit etc. Ignore my comments

I've never done dabs

ShaperDreaming said:

micelio said:

A Twist tie on a loaf of bread

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... I can't remember the last time I saw a twist tie on one of these They're all those shitty plastic clips these days. (Also I'm fucking weird and make my own bread, so I don't pay a lot of attention to that stuff)

But! That's kinda a great idea.

Anyways, whatever ya'll want. I've only gone through 3 inoculation loops in the past 6 months since I got it (I replace when they melt... or just kinda fail when flame sterlizing. Melt isn't a good enough word, maybe pop?).

Fine fine fine fine. Ya'll make them at home. Normally I don't pony up cash for anything, but these were so cheap Seemed silly to go to a vape store, get an extra handle, twist tie that shit etc. Ignore my comments

I've never done dabs

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KapnDank said:

ShaperDreaming said:

micelio said:

A Twist tie on a loaf of bread

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... I can't remember the last time I saw a twist tie on one of these They're all those shitty plastic clips these days. (Also I'm fucking weird and make my own bread, so I don't pay a lot of attention to that stuff)

But! That's kinda a great idea.

Anyways, whatever ya'll want. I've only gone through 3 inoculation loops in the past 6 months since I got it (I replace when they melt... or just kinda fail when flame sterlizing. Melt isn't a good enough word, maybe pop?).

Fine fine fine fine. Ya'll make them at home. Normally I don't pony up cash for anything, but these were so cheap Seemed silly to go to a vape store, get an extra handle, twist tie that shit etc. Ignore my comments

I've never done dabs

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I feel ya man. Personally I love the loop I bought. Came with a bunch of nichrome wire replacements. The way it screws down on the wire makes replacing the wire easy AF! Idk I guess I like it for the same reason I built a flow hood. I just don't like my equipment fighting me lol

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im all abou just buying them shits if I can. I always end up fucking shit up when I try to make it. and im with some of the others. I can't tell ya the last time I bought bread with a twist tie on it.  I also shop at organic markets so that might have something to do with it lol.

OK SO UPDATE!

A couple of the "Z" spread plates are showing the same contams again but not all, so maybe there's hope for 1 or 2 of the syringes!? we'll see in another few days.

on the flip side though, one of my "control" plates is showing signs of growth (pic below).  as you can see the one on the left that I did NOT open is fine... noting seems to be affecting it.  BUT the one on the right (if you recall) I opened it for about 3 seconds inside the SAB and then closed it and its showing growth signs.  thoughts?


SAB Tek
Large clear plastic tub, with holes cut on 1 side.
I lay down a fresh black trash back on the table adn spray it with 70% alcohol then I spray the inside of the SAB adn place it on the table and let both sit for around 5 min.  after that I bring in all of my plates etc and wipe them all down inside the SAB with alcohol and begin the process.