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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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I seriously don't get it.
    #26011093 -

Soaking WBS 24hours
PC'ing 2 hours at 19psi (I'm at 10k feet)
knocking jars In SAB from Agar that looks great.

Green mold

Same methods
syringe Knock inside SAB

Green mold (yes I know this isn't the proper way to do things)

previous to my move my grows were great.  never short of 5 bulk tubs maybe 1 out of a group would go bad.

in the last year now I've only been able to get 2 tubs to grow out of probably 50+ that I've attempted.

everyone say's... its your seed its your seed.. well what do I try now??  cause I've tried everything that has been suggested to me on the forums.


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Re: I seriously don't get it. [Re: 10kVisions]
    #26011102 -

Are you making at least one transfer from agar?


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.-Non sibi sed patriae-.

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Re: I seriously don't get it. [Re: MechaLegend]
    #26011137 -

Multispore or LC?


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Question everything, it's very responsive.
culture storage

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Re: I seriously don't get it. [Re: 10kVisions]
    #26011141 -

I'm guessing your increased pressure isn't enough to compensate for the change in altitude. Easiest test I can think of is running a few jars without inoculating them and seeing if they still trich out.

How many transfers are you doing prior to inoculating your grain?

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Re: I seriously don't get it. [Re: Psilo_citizen]
    #26011143 -

Pics of agar and grain would help. You could always do mini monos to diversify your risk.


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"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:

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Re: I seriously don't get it. [Re: Psilo_citizen] * 2
    #26011145 -

Psilo_citizen said:
I'm guessing your increased pressure isn't enough to compensate for the change in altitude. Easiest test I can think of is running a few jars without inoculating them and seeing if they still trich out.

How many transfers are you doing prior to inoculating your grain?


"Easiest test I can think of is running a few jars without inoculating them and seeing if they still trich out.":rollsafe:


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Question everything, it's very responsive.
culture storage

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Re: I seriously don't get it. [Re: MechaLegend]
    #26011155 -

MechaLegend said:
Are you making at least one transfer from agar?



agar is 3 if not 4 transfers before applying to grains


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Re: I seriously don't get it. [Re: 10kVisions]
    #26011165 -

Actually alot of people will drip to grain after one transfer I have even dropped wiped plates to grain after I transfer a wedge ..


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Re: I seriously don't get it. [Re: Psilo_citizen]
    #26011171 -

i should know what this means but i get lost in all the abbreviations for things on the forum...


I adjusted my PC pressure accord to a couple of people on the forums who helped me do the math.  i've even tried going up to 2.5 hours and same result
as far as letting jars sit without anything. i've done this and nothing happens. jars just sit and seed dries out.

here are my last couple of agar pics.  they sat a little longer than i wanted them too cause i had to go out of town but this is after the 4th transfer.






My agar grows great most of the time. I'm using Pasty Whites agar tek with glass ball jars.  i usually knock 5 to 10 and end up with 2-3 that look good then i transfer 3-4 times depending from the "good" looking myc.  all the growth is "crawly" and clean no "rings" or anything.


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Re: I seriously don't get it. [Re: 10kVisions]
    #26011178 -

Are those your transfer wedges ? If so those are huge Haha.. we shoot for a wedge the size of a grain of rice


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Re: I seriously don't get it. [Re: Boogieman47]
    #26011185 -

ya its hard to do small ones with teh ball mini jars cause of how deep they are.  does that matter though?  if im transferring until i see clean growth wouldn't that be the same?


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Re: I seriously don't get it. [Re: 10kVisions]
    #26011189 -

Small transfers, mean less area for a contam spore to stick on.

I dont like that growth either. Anything that doesn't follow the regular mycelium organization is a red flag.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:

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Re: I seriously don't get it. [Re: 10kVisions]
    #26011191 -

and 2ndly when this all worked great for me (before i moved) i did straightening syringe knocks.  never did agar till now and never had any MAJOR issues... this on the other hand is insane.  it seems not matter what i do i get mold.

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Re: I seriously don't get it. [Re: 10kVisions]
    #26011198 -

It's just your technique .. you need to work on your transfers stop using ball jars to do agar .. if you have pasty plates use those and make smaller transfers ..

If you use spore solution on agar use one or 2 drops on a plate .. but only one in each spot


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Re: I seriously don't get it. [Re: Boogieman47]
    #26011210 -

What's your SAB design like? What's your personal technique, what steps you take to open your ball agar jars and slice the wedge and inoculate the next plate? You wear gloves? Use ISO? Got a torch? What's the size of SAB? HAve a proper scalpel?



Have you run any controls where you don't touch them whatsoever after a PC cycle to see if your jars are still contaminating (if those contaminate, could help pointing towards issue during sterilization like maybe PC isn't reaching the proper PSI, nd issues are unrelated to your incoculant)


Depth of agar plate shouldn't really make much difference in the size of the wedge you can cut. Sure the depth/thickness of it will always be the thickness of the plate but you can even do some slight scalpel moves and slice only the top half of the agar plate wedge off, leaving half the thickness behind. Aim for a grain of rice sized or smaller as mentioned earlier.


With smaller wedges taken from leading edges that you can visually inspect and know have healthy mycelium, you are more likely to be transferring just mycelium and leaving the nasties behind. That's what you want. I wouldn't give up on agar just cause you're having issues, it might seem cumbersome or tricky or a pain in the ass but you need to improve technique and agar will make you very happy. Saves a lot of hassle and money being able to do agar right.

Those plates do look funky to me, but there is good growth there. Try to grab a small ass wedge and make transfer but I would also get some spores germinating on agar again if I were you, just in case you can't get that cleaned up

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Re: I seriously don't get it. [Re: Nichrome]
    #26011215 -

Nichrome said:
Psilo_citizen said:
I'm guessing your increased pressure isn't enough to compensate for the change in altitude. Easiest test I can think of is running a few jars without inoculating them and seeing if they still trich out.

How many transfers are you doing prior to inoculating your grain?


"Easiest test I can think of is running a few jars without inoculating them and seeing if they still trich out.":rollsafe:



Didn't see this post. Yes. Controls are your friend OP.


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Re: I seriously don't get it. [Re: sh4d0ws]
    #26011218 -

I just meant try not to transfer the entire donor plate Haha.. well put sh4dows


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Re: I seriously don't get it. [Re: 10kVisions]
    #26011223 -

Im with those saying it's your agar technique. Those jars don't look like anything id even consider putting to grain. Switch to containers that are easier for you to work with(mini rounds and pp5 deli cups are the most popular as far as im concerned) and take MUCH smaller transfers earlier on.


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"I members.... do you members"
Memberberries circa 2016


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Re: I seriously don't get it. [Re: Psilo_citizen]
    #26011230 -

Actual petri dishes are cheap. I got 500 for around 80 bucks.


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Question everything, it's very responsive.
culture storage

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Re: I seriously don't get it. [Re: sh4d0ws]
    #26011490 -

sh4d0ws said:
What's your SAB design like? What's your personal technique, what steps you take to open your ball agar jars and slice the wedge and inoculate the next plate? You wear gloves? Use ISO? Got a torch? What's the size of SAB? HAve a proper scalpel?



Have you run any controls where you don't touch them whatsoever after a PC cycle to see if your jars are still contaminating (if those contaminate, could help pointing towards issue during sterilization like maybe PC isn't reaching the proper PSI, nd issues are unrelated to your incoculant)


Depth of agar plate shouldn't really make much difference in the size of the wedge you can cut. Sure the depth/thickness of it will always be the thickness of the plate but you can even do some slight scalpel moves and slice only the top half of the agar plate wedge off, leaving half the thickness behind. Aim for a grain of rice sized or smaller as mentioned earlier.


With smaller wedges taken from leading edges that you can visually inspect and know have healthy mycelium, you are more likely to be transferring just mycelium and leaving the nasties behind. That's what you want. I wouldn't give up on agar just cause you're having issues, it might seem cumbersome or tricky or a pain in the ass but you need to improve technique and agar will make you very happy. Saves a lot of hassle and money being able to do agar right.

Those plates do look funky to me, but there is good growth there. Try to grab a small ass wedge and make transfer but I would also get some spores germinating on agar again if I were you, just in case you can't get that cleaned up


OK so as stated in the first thing i posted.  this is NOT my first rodeo. I've been at this for over 5 years now and have had VERY successful grows...
SO,
gloves, SAB (big tub with holes cut Bodhi's tek), torch, exacto knife, yes i use the torch on every scalpel between anything im working with along with wiping it with alcohol after torched and letting git sit in alcohol after using it (watch bodhi's videos)

transfers are simple, put 4 jars into sab, loosen lids but not remove, open agar cut wedges.  remove wedge (after torch and alcohol wipe) then lift lid and drop wedge in. 

i got better grows knocking straight from syringes in ALL my previous grows up until now.

so what changed?

the altitude. i went from 5 to 10k feet.  so i have to adjust my PC temp to make up the difference.
Is 19psi for 2 hours not long enough?  according to a few mathematicians on here that's what i should be doing.  if anyone thinks im not going long enough then please break out the math and let me know im open to ANY suggestions.

I knocked with staring syringes the last go around alone side my Agar and got the same result, i did this just to try it out cause i was so successful with it previously.

what im not understanding about the agar is that what im transferring looks great.  has the crawly growth etc.. even these looked like that before becoming overgrown after sitting too long.


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