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Invisiblemicelio
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Registered: 04/22/14
Posts: 1,547
Re: I seriously don't get it. [Re: 10kVisions]
    #26014288 - 05/26/19 12:24 PM (4 years, 9 months ago)

Quote:

Professor X said:
Have you done drop plates in your home? Sounds like an extreme spore load. Excuse me if it's extremely hard to believe that mp tape is giving you a 0.1% success rate. If that is the case it's probably an environmental issue.




My 2 cents..
The problem is you'll not visually seeing Trichoderma until It pops up it's ugly head from deep down in your sub...
What I was trying to say earlier is you don't need filters in your jelly jars. You can cover your 1/4" hole on top of your plastic lid with masking tape. Then back off the lid a quarter turn for the pc cycle to equalize the pressure. Tighten the lids after pc run is finish and dishes are still warm. It doesn't get simpler than that. Or you can use your micropore tape for the pc cycle and cover the microspore tape with masking tape. The masking tape is to keep the unwanted green out..
There's plenty of air in your jelly jars, you don't need gas exchange..
Also you may want to consider bringing in some new spore print from a outside source.. I too used micropore tape until I notice mold under the filter hole. More than once..


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Invisible10kVisions
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Registered: 04/08/18
Posts: 188
Re: I seriously don't get it. [Re: Professor X]
    #26014295 - 05/26/19 12:29 PM (4 years, 9 months ago)

ya Im at such a loss at this point that im trying literally anything that might give me results.  no hate, no "UGH WHY" or anything... give me advice, I'll give it a shot lol. I love doing this but man is it frustrating when you run into problems as big as mine.  im really hoping it turns out to be something simple like MP Tape and I can just smack myself and get going again.

and now boys and girls, our best friend! Patience


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InvisiblemushboyMDiscord
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Re: I seriously don't get it. [Re: 10kVisions] * 4
    #26014296 - 05/26/19 12:31 PM (4 years, 9 months ago)

Step 1: dont listen to prof x
Step 2: change filters
Step 3: review sterile tek
Step 4: grow shrooms.

Easy

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Invisible10kVisions
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Registered: 04/08/18
Posts: 188
Re: I seriously don't get it. [Re: mushboy] * 1
    #26014307 - 05/26/19 12:38 PM (4 years, 9 months ago)

Quote:

mushboy said:
Step 1: dont listen to prof x
Step 2: change filters
Step 3: review sterile tek
Step 4: grow shrooms.

Easy




lol nice.

1. thats between you guys
2. working on that as we speak
3. reviewed and following closely
4. fingers crossed!
cheers


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Invisiblemicelio
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Registered: 04/22/14
Posts: 1,547
Re: I seriously don't get it. [Re: 10kVisions]
    #26014685 - 05/26/19 04:43 PM (4 years, 9 months ago)

Hey..!
My fingers are also cross for you.. I know the feeling...

There's not many places in the US. where you can live at 10,000 ft.

John Holliday started Aloha Medicinals in Carson City Nv. because of the 6,000 ft. altitude that has ideal climate conditions for growing mushrooms.


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InvisibleJHOVA
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Re: I seriously don't get it. [Re: 10kVisions]
    #26015337 - 05/27/19 12:56 AM (4 years, 9 months ago)

Quote:

10kVisions said:
so another thing i would like to point out is that the Pasty White tek for agar calls for MP tape.  hence why i was using it.  following his tek exactly and just adjusting the PC pressure for my alt.




Pasty plates have an entire sheet of paper towel folded up and placed over the top. Its not mp by itself.


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Invisible10kVisions
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Registered: 04/08/18
Posts: 188
Re: I seriously don't get it. [Re: JHOVA]
    #26016456 - 05/27/19 02:38 PM (4 years, 9 months ago)

Quote:

JHOVA said:
Quote:

10kVisions said:
so another thing i would like to point out is that the Pasty White tek for agar calls for MP tape.  hence why i was using it.  following his tek exactly and just adjusting the PC pressure for my alt.




Pasty plates have an entire sheet of paper towel folded up and placed over the top. Its not mp by itself.




ya I noticed that.  I always assumed he removed it but upon looking over his tek again I saw that he never removes it.  Once again pointing out the teh MP Tape is becoming more and more likely my issue.

appreciate the input.


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Invisible10kVisions
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Re: I seriously don't get it. [Re: 10kVisions]
    #26016486 - 05/27/19 02:52 PM (4 years, 9 months ago)

UPDATE:


The Agar Setup  and PC.


Purging


PC Temp



Jars PC'd at 19psi for 2 Hours. Plain, no knock.  we'll see if anything grows


Agar using Polyfil instead of MP Tape.  PC'd 19psi for 45 min.  knocked with BHT exactly teh same way I did the set with MP Tape on them.


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OfflineRaccoon
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Registered: 04/08/19
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Re: I seriously don't get it. [Re: 10kVisions]
    #26016847 - 05/27/19 06:21 PM (4 years, 9 months ago)

Good luck, brotha.


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Invisible10kVisions
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Posts: 188
Re: I seriously don't get it. [Re: Raccoon]
    #26017170 - 05/27/19 09:07 PM (4 years, 9 months ago)

Quote:

Raccoon said:
Good luck, brotha.




LOL!  thanks!


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Invisible10kVisions
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Posts: 188
Re: I seriously don't get it. [Re: 10kVisions]
    #26025610 - 05/31/19 06:51 PM (4 years, 9 months ago)

UPDATE:

WBS Plain (no knock) jars
so far the jars aren't growing anything both PT and Polyfil jars look the same. so thats good news.  at least i know the PC'ing is doing its job.

Agar
The Polyfil jars are developing faster than the PT jars are for sure. i've seen the same ammout of growth that took 5 days in the PT jars happen in only 2 days in the Poly jars.  agar is the same... technic, PC all that is exactly the same.
Here are the Agar Photos so far.



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InvisibleJHOVA
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Re: I seriously don't get it. [Re: 10kVisions]
    #26025671 - 05/31/19 07:16 PM (4 years, 9 months ago)

take a pic like this inside your sab. its easier if you have the lid on a cookie rack upside down.


i cant tell if you have contams or not from the pics. :cookiemonster:


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Invisible10kVisions
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Registered: 04/08/18
Posts: 188
Re: I seriously don't get it. [Re: JHOVA]
    #26025682 - 05/31/19 07:22 PM (4 years, 9 months ago)

Quote:

JHOVA said:
take a pic like this inside your sab. its easier if you have the lid on a cookie rack upside down.


i cant tell if you have contams or not from the pics. :cookiemonster:




good call i'll work on that tomorrow


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InvisibleMad Season
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Re: I seriously don't get it. [Re: 10kVisions] * 1
    #26026799 - 06/01/19 09:11 AM (4 years, 9 months ago)

Those look so fuckin sketch. Doesn't even look like cube myc to me..


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OfflineProfessor X
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Registered: 04/18/19
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Re: I seriously don't get it. [Re: Mad Season]
    #26026804 - 06/01/19 09:16 AM (4 years, 9 months ago)

Sure doesn't look like cube myc. It looks like trichoderma. What are you inoculating with? Might want to start completely fresh, new prints.

By the way, waaay too much agar in those dishes. Just barely cover the bottom.

Cube mycelium doesn't grow all weird on agar like that it grows in circle like formations.


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Invisiblemicelio
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Registered: 04/22/14
Posts: 1,547
Re: I seriously don't get it. [Re: 10kVisions] * 2
    #26026852 - 06/01/19 09:36 AM (4 years, 9 months ago)

Quote:

10kVisions said:
UPDATE:

WBS Plain (no knock) jars
so far the jars aren't growing anything both PT and Polyfil jars look the same. so thats good news.  at least i know the PC'ing is doing its job.

Agar
The Polyfil jars are developing faster than the PT jars are for sure. i've seen the same ammout of growth that took 5 days in the PT jars happen in only 2 days in the Poly jars.  agar is the same... technic, PC all that is exactly the same.
Here are the Agar Photos so far.





I assume These pics are growth for three to four days.
To me It looks like bacteria on green agar.. It's hard to tell for sure what I'm looking at.  Just my 2 cents..

:shrug:


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InvisibleMad Season
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Re: I seriously don't get it. [Re: micelio] * 1
    #26026906 - 06/01/19 10:10 AM (4 years, 9 months ago)

Yeah that is bacteria. There's also molds in there too. Look at pic 3. Sterile tek is obviously lacking here.


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

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InvisibleJHOVA
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Re: I seriously don't get it. [Re: Mad Season]
    #26026963 - 06/01/19 10:36 AM (4 years, 9 months ago)

Its too fast to have that much growth. Mycelium grows kinda slow from a tiny wedge.


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Invisiblemicelio
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Registered: 04/22/14
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Re: I seriously don't get it. [Re: Mad Season]
    #26026986 - 06/01/19 10:51 AM (4 years, 9 months ago)

Good eye on pic. 3 Mad season..
Pic. 1&2 has poly fill filter. Pic. 3&4 Micropore tape.
On pic. 3 the mold spores fell right through the Micropore tape as if it was the Grand Canyon.. If people are going to use Micropore tape on their dishes, I suggest to put each dish into a zip lock sandwich bag while they're hot right out of the pc.


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OfflinecronicrFacebook
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Re: I seriously don't get it. [Re: micelio]
    #26027111 - 06/01/19 12:41 PM (4 years, 9 months ago)

The mold is coming from technique or filters/ innoculant, molds die even at pasteurizing temps.


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I'm tired do me a favor

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