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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: I seriously don't get it. [Re: KapnDank] * 1
    #26038669 -

That's not a great sign that your "3 second rule" plate is growing things. That means you're getting contam in your SAB.

I don't know about a towel, but a wire rack seems like a better idea. If your contam falls to the ground and gets locked into a wet towel, then anything that touches that is contamiated. If you use one put a wire rack (for like cooling cookies) over it. Keep your shit away from contam.


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My Shoebox Factory Beginner's Tek | Shoebox Assembly Tek | Methodology/teks I use | How I Store Bulk Items

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Re: I seriously don't get it. [Re: ShaperDreaming]
    #26038691 -

Why not both

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Re: I seriously don't get it. [Re: gizmo1]
    #26038711 -



I use a towel and a cookie rack.


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Re: I seriously don't get it. [Re: JHOVA]
    #26038717 -

I need to make my arm holes bigger. Small ones cause contam problems.

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Re: I seriously don't get it. [Re: gizmo1]
    #26038743 -

gizmo1 said:
I need to make my arm holes bigger. Small ones cause contam problems.


Same.

Also, I was trying to recommend both, but don't use a wet towel myself.


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My Shoebox Factory Beginner's Tek | Shoebox Assembly Tek | Methodology/teks I use | How I Store Bulk Items

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Re: I seriously don't get it. [Re: ShaperDreaming]
    #26038877 -

hominy, coffee, tomato, and paint cans should do the trick. whatever is available.


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Re: I seriously don't get it. [Re: JHOVA]
    #26038886 -

Seems like big metal cans are getting more and more rare everyone is going to plastic. I cut mine with a wood burner and cutting tip but ive also used a dremel harbor freight sells a nifty little dremel type tool you can catch on sale for like 8 bucks.

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Re: I seriously don't get it. [Re: gizmo1]
    #26038987 -

big lots or mexican/ethnic market has em 100%


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Re: I seriously don't get it. [Re: JHOVA]
    #26039140 -

I recently had three excellent-looking oat jars with vigorous, healthy growth.  I decided to use each of them for G2G.  One by one, on separate days and using my SAB, I dumped a parent into three receiving jars for a total of nine receiving jars.

Every. Single. One. Contaminated.

I thought what the hell could have happened?  I kept going over my technique to try to identify what could have possible resulted in such a catastrophic fucking failure.  Nothing ever stood out to me, so I chalked it up to terrible luck, or the polyfill lids, or some kind of sneaky mold that didn't reveal itself until late in the game. 

The thing is that I always leave just a little bit of oat in the parent jar when doing G2Gs in order to see if anything funky grows out.  Nothing ever did in any of the parent jars in this case - so I knew it had to be something that I did during the G2G transfer.

Well, while getting ready to do some agar work yesterday I began pulling all of my gear out of my tub.  I use pieces of a cut-up t-shirt to dip into 70% alcohol and wipe down equipment, jars, etc., and when I'm finished with them I cram them into a plastic petri dish sleeve to keep them clean.  Well, this time, when I pulled that bag out, I immediately smelled mold.  I reached in, pulled out the damp t-shirt scraps, and recognized little green dots of mold covering them.

Turns out that the one thing I thought was clean (because it was soaked in alcohol when in use) and that was being rubbed all over my jars and tools had actually become a fucking contam nightmare.

This is all to say that when it comes to a reoccurring contam problem, it is necessary to examine every minute detail of the process - or perhaps to even start completely from scratch.  In my case, if the damp rag-in-a-bag hadn't started to show little dots of mold, who knows how much longer I would have continued to rub mold all over everything before recognizing the source of the problem.

Good luck!

Edited by teaker (06/07/19 09:10 PM)

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Re: I seriously don't get it. [Re: teaker]
    #26039180 -

Did you purge all the air out of your pressure cooker. I always go 15 min. from the time I see steam and hear the water at a rapid boil.
I also always go 2 hours on my steam cycle for grain jars..
Air in the pc acts as a insulator.


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Re: I seriously don't get it. [Re: KapnDank]
    #26040142 -

KapnDank said:
I also agree with the wet towel. I used to lay one down soaked with 10% bleach solution. If the spore load is high in your house it wouldn't hurt to get some Ozium, not Lysol you want something with Triethylene Glycol(TEG),  and spray around your work area to kill some of the spores floating around. Make sure there's no moving air in the room. Fans, air conditioner, hyperactive cat:lol:, etc


thanks everyone.  regardless of peoples Tek's allot of them aren't very thorough about the setup... just what to use and how to use it.
the room I'm in i buit, its dead.  4 walls and ceiling. no fans no windows etc.  one door in and out so threre is virtually NO air movement in it.  i have another control idea to do inside the sab.

So:
After i knocked all the individual syringe jars and when i was done with those and they were no longer in the SAB i took out one of my contaminated agar jars and did the transfer into another clean agar.  THEN i opened the untouched agar jar and closed it.  im 99% sure the contams i have inside the control jar came from the contaminated jar i had opened.
another idea to test that i to take 2 more control jars and reset the SAB (alcohol etc) then open them inside without anything else and see if the growth happens again.


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Re: I seriously don't get it. [Re: 10kVisions]
    #26040428 -

Hey..!
You can run a steam cleaner in the room you where describing for 12 hours and problem solved..


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Re: I seriously don't get it. [Re: micelio]
    #26040439 -

micelio said:
Hey..!
You can run a steam cleaner in the room you where describing for 12 hours and problem solved..


steam cleaner for what?  theres no carpet or anything.  linoleum floors....


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Re: I seriously don't get it. [Re: 10kVisions]
    #26040560 -

10kVisions said:
micelio said:
Hey..!
You can run a steam cleaner in the room you where describing for 12 hours and problem solved..


steam cleaner for what?  theres no carpet or anything.  linoleum floors....


I'm not talking about a carpet cleaner..Ha..
A steam generator It put out actual steam.. From what I understand is mushroom farms with contamination problem run the steam generators in their grow area. I imagine all tool and equipment rental would rent one.
They use steam cleaners like a pressure washer to clean parts and engines that all greasy..
I myself wouldn't hesitate to use one to kill all contaminants in every nook and cranny.. What you do is fill the room with steam for a period of time.. Sometime you just have to start over from the ground up and try not to let the mold spores and bacteria get out of hand..


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Re: I seriously don't get it. [Re: micelio]
    #26040635 -

micelio said:
10kVisions said:
micelio said:
Hey..!
You can run a steam cleaner in the room you where describing for 12 hours and problem solved..


steam cleaner for what?  theres no carpet or anything.  linoleum floors....


I'm not talking about a carpet cleaner..Ha..
A steam generator It put out actual steam.. From what I understand is mushroom farms with contamination problem run the steam generators in their grow area. I imagine all tool and equipment rental would rent one.
They use steam cleaners like a pressure washer to clean parts and engines that all greasy..
I myself wouldn't hesitate to use one to kill all contaminants in every nook and cranny.. What you do is fill the room with steam for a period of time.. Sometime you just have to start over from the ground up and try not to let the mold spores and bacteria get out of hand..


This sounds like it would cause mold hot moist air and all. You sure you arent thinking of a fog machine ive heard the fog solution is a disinfectant and I think someone here had a tek for building a fpg machine for growing.

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Re: I seriously don't get it. [Re: gizmo1]
    #26040685 -

Perhaps a fog machine using TEG?:shrug:


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Re: I seriously don't get it. [Re: KapnDank]
    #26040698 -

KapnDank said:
Perhaps a fog machine using TEG?:shrug:


Im not sure what that is did a quick search on the web couldnt find much about it but I found some links that led me to dissinfectant  foggers whos going to order and try them out OP? Lol

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Re: I seriously don't get it. [Re: gizmo1]
    #26040728 -

I'll try this again. If 10k has no windows one door and the room is seal up good with no air draft and If you could pump straight steam into the room for 12 hours there will be nothing living in that room. You can make a generator out of a fifty gallon drum and a big propane burner under the drum.
Drake89 TC. Has a edible mushroom farm. He has one room that he loads up with racks of bags for growing edibles. He pipes in the steam for twelve or more hours to sterilize the bags of sawdust.. It's one atmosphere sterilizing.


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Re: I seriously don't get it. [Re: micelio]
    #26041030 -

Is it likely that the room is set up to handle excessive moisture like chemlite sheeting overtop of mold resistive coating overtop of green mold resitant sheetrock? Take a bathroom coated in sheet rock for instance over time the excessive amounts of steam will cause mold growth. In all my years in construction I have never demolitioned a room exposed to constant steam that didnt have mold growth even if it wasnt visible. Ever torn out a shower kit or tile in a bathroom?

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Re: I seriously don't get it. [Re: gizmo1]
    #26041033 -

Wtf is demolitioned I hope thats a real word lol.
Edit: ignore what im saying mold on the wall doesnt matter when it comes to what your talking about. It also wont benefeit op as soon as steam stops being pumped into room its no longer sterile no point in arguing that lol.

Edited by gizmo1 (06/09/19 12:14 AM)

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