Olympus said:

I’ll put this here since it’s related.

I have not been able to get full colonization to oats. I’m assuming spore syringe to grain is just a bad technique.  I’ve recently started an agar to grain run so I will be able to conclude my theory soon.

I’m assuming these are bacterial. G2G transferred this batch 2 weeks ago. Shook 1 week ago at 25%+/-.  So far the myc runs look good until I shake. They recover quickly after the shake and then stall out and will stay in this half colonized state.

Have yet to see any contamination in my agar with the same syringe.

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That doesn't look "half colonized to me." It might be slightly bacterial. Compare to mine in this thread. The density looks similar, except mine has more bacterial nastiness. The fact that it recovers quickly means it's okay in my book. Spawn to a shoebox and find out. Update the thread when you get those sweet mushies

ShaperDreaming said:

Just going to pop in and say that I don't consider minor bacteria to be a "tossable" offence. I spawn bacterial shit all the time and just put it in a box with a frowny face drawn on it. It has yet to affect most of my yields, even when letting them go out to 3-4 flushes, they do fine.

There is an unacceptable level of bacteria, when it's pooling yellow or preventing colonization in larger-than-part-of-a-grain radius then I avoid them. A little bacteria is kinda in everything doesn't mean it's going to fuck mush up. If I was running a commercial mushroom farm I may be giving different advice.

I always tell people to spawn that shit while noting that it is bacterial so they know what it looks like. Good work racoon, I would have expected as much if I spawned them jars. How many flushes are you getting?

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Raccoon said:

I'm struggling to get the conditions correct with my second flush and it looks stalled. It has not contamed yet, but the conditions were atrocious. Wet and dry at the same time. They were pretty dry after harvest so I dunked 2 tubs (not long enough) and heavily misted the others. I kept having to mist the tubs because they would dry out. The surface was sopping wet, all the pins looked glistening for a few days, the condensation on the lids was non existent, and the sub looked dry after a few hours. Yesterday I decided to bottom soak them all (pouring water down the side and draining after a few hours). Now the conditions look pretty good. I assume the pins will abort (if they haven't already), and I hope new pins will start.

I live in the high desert, so it's pretty dry. I think I got lucky by accidentally going slightly over field capacity on my first flush. Dunking properly will probably be necessary for subsequent flushes in the future.

There's no flush tek After this debockle I think I got it figured out but it'd be nice to not have to fail to learn.

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Raccoon said:

I live in the high desert, so it's pretty dry. I think I got lucky by accidentally going slightly over field capacity on my first flush. Dunking properly will probably be necessary for subsequent flushes in the future.

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So, here's how I do shoeboxes, but I live in the mid-west, so I have 40% RH most of the year. But I find that the best route for these is a set-and-forget route. If you're fussing with them more than once every 3-5 days, try a different approach.

What would you want to see in a "flush tek"? I'm still working on the above tek, and plan to include a soaking section for what to do between flushes. This is how long I soak, but people seem to think I'm doing it too long. Screw it, this works for me.

Olympus said:

Here are a couple pictures of the shoeboxes. This is 10 days in I popped the lid to photo. They are currently at day 14 and no signs (that I know of) of pinning.  No  pseudo top layer but I will next time.



Is the grain stalling issue caused by bacterial or simply multi strain genetics via spore syringe? Does this translate to inability to pin or slow to pin?

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Olympus said:

My Current theory: Do multi strain genetics lead to mycelium stress, which causes jars to stall, which then promotes a bacterial environment?

This is where my inexperienced beginner deductive reasoning has led me.

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