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Agar possible contam?
    #26002290 -



Is that mold or something besides the bottom left little myc?


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Re: Agar possible contam? [Re: Enkidu]
    #26002374 -

Not sure exactly what you mean, what do you think is mold? It's hard to tell from that picture, I can see some healthy myc in there, was this a clone or agar to agar transfer? It seems like there's a pretty big mass in the middle that the myc is growing off of, it kind of looks like there may be some bacteria in the middle or something possibly.

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Re: Agar possible contam? [Re: do.what.you.want]
    #26002399 -

It's agar to agar

Guess I wasn't sure if the growth was all myc or only the rhizo was


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Re: Agar possible contam? [Re: Enkidu]
    #26002429 -

It looks like mycelium all around the blob in the middle, how big was the transfer you took out of the previous dish? I try to take transfers no bigger than a dry grain of rice. Pretty sure I got that from RR sometime back.

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Re: Agar possible contam? [Re: Enkidu]
    #26002465 -

Bacteria. Best bet is to take a very very small transfer from the edge of the one ropey growth you have.


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Re: Agar possible contam? [Re: southbounpachyderm]
    #26002474 -

Will do next time

Might just toss the plate, not sure yet


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Re: Agar possible contam? [Re: Enkidu]
    #26002502 -

Take a sample between 8 and 9 from that nice sector.


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Re: Agar possible contam? [Re: Enkidu]
    #26002589 -

It's not a toss imo just make sure you take the edge from between 8 and 9 like said above. specifically where the rhizo quicker growth is. It's out running the bacteria there.


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Re: Agar possible contam? [Re: southbounpachyderm]
    #26002640 -

I agree, I see a clean isolated strain there, just need to transfer again.


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Re: Agar possible contam? [Re: Enkidu]
    #26002937 -

I wouldn't toss it, take a transfer from that rhizo growth, one main reason we do agar is to clean up cultures. if you have a flow hood don't take these transfers infront of it, just use a s.a.b, or even just a clear garbage bag.

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Re: Agar possible contam? [Re: Professor X]
    #26002939 -

Not isolated, but it's in the process of being isolated.

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Re: Agar possible contam? [Re: do.what.you.want]
    #26002966 -

Ok cool I'll do some work with it

Thanks guys


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Re: Agar possible contam? [Re: do.what.you.want]
    #26003066 -

do.what.you.want said:
Not isolated, but it's in the process of being isolated.


If he takes the sample where we told him he will have an isolated strain (single sector).


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Re: Agar possible contam? [Re: Professor X]
    #26005627 -

What are the benefits of that?

Consistency?


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Re: Agar possible contam? [Re: Enkidu]
    #26005631 -

Professor x you're wrong that sector still consists of lots of strains no where near an isolate  .. that plate is fine man dont worry how many transfer are you from the original plate ??


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Re: Agar possible contam? [Re: Boogieman47]
    #26005634 -

thats one transfer out from the original plate i do believe.

Either 1 or maybe two actually.

I cant remember :lol:


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Re: Agar possible contam? [Re: Enkidu]
    #26005638 -

Ok it looks like Ms to me ? If so that's why you have so many crazy growth patterns you have tons of strains fighting on that plate Haha.. dont stress on it just keep transferring the best looking edges and keep on rolling


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Re: Agar possible contam? [Re: Boogieman47]
    #26005646 -

It was an old tissue sample to agar, then I think I transferred it once, maybe twice, can't remember

Its been sitting in my fridge for about a year now :lol:

I just got some some prints of RW recently and I have some other tissue samples starting to grow on agar and about 4 more of these old plates pulled out, i plan to transfer all of them over the next couple weeks or so as they begin growing out


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Re: Agar possible contam? [Re: Enkidu]
    #26005667 -

For sure thats cool it's still viable after a year .. usually you see that off shoot growth from Ms more then clones but clones will push like that as well sometimes also .. man searching for clones is a pain huh??


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Re: Agar possible contam? [Re: Boogieman47]
    #26005681 -

Im just sick of having jars that everyone tells me are bacterial

And these last ones seem to have wet grains and wet spots

Thinking of switching from WBS and trying oats

Sucks

Hoping agar just helps give me some clean spawn for once

Maybe i've never seen clean spawn in my life before


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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