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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: blindingleaf]
    #19351449 - 12/31/13 02:55 PM (10 years, 29 days ago)

Quote:

blindingleaf said:
pasty i blame u for having too many plates to work with.






:lolsy: Welcome to my world :lol:

Quote:

blindingleaf said:
what is ur opinion on taking a wedge from the edge of these PP5's vs a regular petri?  am i just lucky or are the higher walls offering a better barrier?




I have done it and not experienced signigificant issues, not sure if I was just lucky or what. Most bacteria spreads through contact and I find the little handle on the lids helps keep me from touching the lids too close to where they close. This might be the reason for my luck with transfers near the edge :shrug: Its probably not the best practice but its not certain doom if you do.


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InvisibleHypnotoadCroaked
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19351631 - 12/31/13 03:44 PM (10 years, 29 days ago)

I have been quite surprised with how little "edge" contamination I have had.  The only times I have seen bacteria around edges is when I was working with OD prints and clone flesh.  I have yet to see any random "Hey I did not put that there".  I dunno what I am doing right, but thus far agar has been a god-send.  I have had no issues letting the mycelium poke up the sides, and simply just rubbing a few "feather ends" and poking them into another dish. 

Mileage may vary.


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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19351661 - 12/31/13 03:52 PM (10 years, 29 days ago)

yea i seem to have better luck with agar work than g2g.  none of my wedge to grain jars contam ever, but one in 15 jars of g2g will contam.  i am using postal tyvek on these, one layer over the lid, secured down by rim, and have done this with success before.  i think i need a taller SAB because many times, the last of the master jars grain will be difficult to shake in the last g2 jar without jostling around a lot in the say, and letting the two jar rims touch. can't wait to get electric drill to make the plastic lids.  i have sfd's but only six, so when they are in use, i go tyvek until i can turn those six into 36


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MICROBIAL HUSBANDRY!!!!

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InvisibleMudaFuka
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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19351675 - 12/31/13 03:57 PM (10 years, 29 days ago)

this thread is awesome. I never even new about the twist top PP5s before. they are way better to work with than the pop tops.
EDIT I no longer prefer screw tops I posted this back before I knew what the fuck I was doing.


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AMU
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Liquid Inoculant Tek                                   
                     


Edited by MudaFuka (08/23/14 12:27 AM)


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OfflinecronicrFacebook
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Re: Pasty Agar Tek [Re: MudaFuka]
    #19351686 - 12/31/13 04:00 PM (10 years, 29 days ago)



i've been using these lately, just to germinate for the most part but there nice and small and i don't waiste alot of agar like i feel i do with the big twist tops


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleMudaFuka
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Re: Pasty Agar Tek [Re: cronicr]
    #19351842 - 12/31/13 04:45 PM (10 years, 29 days ago)

Quote:

cronicr said:


i've been using these lately, just to germinate for the most part but there nice and small and i don't waiste alot of agar like i feel i do with the big twist tops



do you have to add any holes to those things for GE


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     


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OfflinecronicrFacebook
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Re: Pasty Agar Tek [Re: MudaFuka]
    #19351858 - 12/31/13 04:49 PM (10 years, 29 days ago)

yep theres a hole with poly there:thumbup:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflinecronicrFacebook
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Re: Pasty Agar Tek [Re: cronicr]
    #19351863 - 12/31/13 04:50 PM (10 years, 29 days ago)

and pasty i'm gonna try get this added to the info section if you don't mind:thumbup:
gathering links up right now for some updated teks


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: cronicr]
    #19351916 - 12/31/13 05:02 PM (10 years, 29 days ago)

Quote:

cronicr said:
and pasty i'm gonna try get this added to the info section if you don't mind:thumbup:
gathering links up right now for some updated teks



Not at all, more exposure = more people doing agar :awesomenod:


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InvisibleViolet
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Re: Pasty Agar Tek [Re: cronicr]
    #19352715 - 12/31/13 08:37 PM (10 years, 29 days ago)

Quote:

cronicr said:


i've been using these lately, just to germinate for the most part but there nice and small and i don't waiste alot of agar like i feel i do with the big twist tops




20-25mL, just like petris...
500mL still makes 20+


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
• Violet's Teks and Posts •


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OfflinecronicrFacebook
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Re: Pasty Agar Tek [Re: Violet]
    #19352719 - 12/31/13 08:40 PM (10 years, 29 days ago)

yes i could have said the twist tops and petris and or any of them lol, i like the tiny ones to start on, good watching out violet and happy new years:thumbup:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleViolet
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Re: Pasty Agar Tek [Re: cronicr]
    #19352726 - 12/31/13 08:41 PM (10 years, 29 days ago)

Happy new year chron, pasty, everyone!


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
• Violet's Teks and Posts •


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OfflineForresterM
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Re: Pasty Agar Tek [Re: Violet]
    #19353126 - 12/31/13 10:57 PM (10 years, 29 days ago)

:rockon: :headbang3: :rockon:


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Re: Pasty Agar Tek [Re: Pastywhyte] * 2
    #19354743 - 01/01/14 01:30 PM (10 years, 28 days ago)

Quote:

Pastywhyte said:
Finally I spoon in the agar mix into my glad mini rounds. I like to just give them a wipe with a damp paper towel first to clear out any dust or debris. No need to be spraying them with alcohol or nothing. Place the lids on the top and close. Finally I like to fold up a square of paper towel and place on top. Condensation can be a bit of an issue and you don't want to get the micro pore tape too wet. The paper towel helps with this. I have found as well that the paper towel will be too wet if you bring the pressure up to fast in the PC, which will lead to the agar boiling up on the sides. This is a great indication of how good your technique is for this. Here is the agar in the PP5's and how I do the paper towel.




hey bro you should try letting your agar get to room temp and firm before putting your lid on and loading, helps a bunch with the condensation, i barely ever get any:thumbup:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Edited by cronicr (01/01/14 01:37 PM)


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OfflineForresterM
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Re: Pasty Agar Tek [Re: cronicr]
    #19354845 - 01/01/14 01:59 PM (10 years, 28 days ago)

Quote:

cronicr said:
hey bro you should try letting your agar get to room temp and firm before putting your lid on and loading, helps a bunch with the condensation, i barely ever get any:thumbup:




No shit?  I gotta try that.  So you heat it up to dissolve everything in, then let cool, then PC?


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Re: Pasty Agar Tek [Re: Forrester] * 1
    #19354852 - 01/01/14 02:01 PM (10 years, 28 days ago)

yep:thumbup:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Re: Pasty Agar Tek [Re: cronicr]
    #19354855 - 01/01/14 02:02 PM (10 years, 28 days ago)


i've been getting none with my spice jars and very little/none on my pp5's


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineForresterM
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Re: Pasty Agar Tek [Re: cronicr]
    #19355110 - 01/01/14 03:27 PM (10 years, 28 days ago)

Nice!  I'll give it a try next time I do mine :cool:


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: cronicr] * 1
    #19355333 - 01/01/14 04:27 PM (10 years, 28 days ago)

Quote:

cronicr said:
hey bro you should try letting your agar get to room temp and firm before putting your lid on and loading, helps a bunch with the condensation, i barely ever get any:thumbup:




I totally agree, I actually do this already, I guess that was a little something that got overlooked in the writeup and will be adjusted, thanks cron :super:

Actually I will be making a few revisions/additions in a little bit. An alternative to micropore tape has been found that works quite well, I have done a few runs with it now and no issues, so I will be adding that in for the people that have a hard time finding micropore tape. Just need to recover from last night :lol: Ate a lot of stones :wink:

Happy New Year everyone!


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19355432 - 01/01/14 04:50 PM (10 years, 28 days ago)

:thumbup:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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