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thegoonking
Stranger
Registered: 05/11/16
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Last seen: 1 month, 18 days
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newbie here. I have some spore prints on foil.
Can I not use this tek to activate them? Like in a still box, scrape some spores into the agar and let the spores activate and check for contam?
After that, I was going to use blenderless liquid tek.
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tryptkaloids
Learner



Registered: 02/08/15
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Flame sterilize an inoculation loop, cool it in your agar, wipe the loop onto print to pick spores up, swipe across agar in a z pattern.
Good luck!
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Kinoko314
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Registered: 12/16/22
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You can also use a sterile swab the same way (except for the flame sterilizing ). You can PC q-tips after wrapping them in foil if you don't have sterile swabs.
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thegoonking
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Registered: 05/11/16
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Last seen: 1 month, 18 days
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excellent. will try this today. my agar is all cooled off now
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vaudvilleviktor
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#28591215 - 12/20/23 11:21 AM (1 month, 7 days ago) |
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Quote:
Pastywhyte said: I then spoon 2 heaping tablespoons into the pot. I rarely weigh it out, if I want stiffer agar I use a little more, if I want softer I use a little less. If you want to weigh it, for this amount of agar you will want about 2-2.5 grams depending on how soft or firm you want it to be. If your using powdered agar and don't have a scale, a level tsp (5ml) is roughly 2.5 grams. Remember that no matter how much you want to mix up, you want a ratio of 20-25 grams of agar to 1 liter of water. If you have issues with the mix being too stiff while mixing then adjust the ratios for less agar. Some food grade agars will work out to be a little stiffer than others, some experimentation may be required.
two heaping tablespoons or teaspoons?
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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In the tek he uses solid agar so he meant tablespoons, but that's quite a bit for powder
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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shronoob88
Stranger
Registered: 11/14/23
Posts: 43
Last seen: 1 hour, 36 minutes
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Would this be a suitable size for this tek please can anyone recommend?
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lionmane
Edible with Side Effects


Registered: 09/02/19
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Re: Pastywhyte's Easy Agar Tek [Re: shronoob88]
#28596329 - 12/24/23 08:39 AM (1 month, 3 days ago) |
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That size looks good to us. PP5 plastic code, check. Just make sure the lid snaps on securely, cannot ascertain that feature from the photo. Happy Tissue work!
-------------------- -Absence of evidence is not evidence of absence-
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Tiamo
Trust in LITFA




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Re: Pastywhyte's Easy Agar Tek [Re: lionmane]
#28596680 - 12/24/23 02:40 PM (1 month, 3 days ago) |
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3.1 cm is very small for the diameter of the plate. I have 5.5 cm plates and I wouldn't go smaller.
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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shronoob88
Stranger
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Re: Pastywhyte's Easy Agar Tek [Re: Tiamo]
#28600801 - 12/28/23 11:30 AM (30 days, 15 hours ago) |
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Thank you very much for this tek. I managed to find some very cheap tubs, £1.50 for 8 . Seem quite sturdy too so should last a few runs . So the condensation on the side won't matter too much I wouldn't think?
Anyway great tek very easy . 👌

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lionmane
Edible with Side Effects


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Re: Pastywhyte's Easy Agar Tek [Re: shronoob88]
#28601873 - 12/29/23 07:05 AM (29 days, 19 hours ago) |
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Condensation is not an issue. Have fun with your tissue work!
-------------------- -Absence of evidence is not evidence of absence-
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Thankful
Seeker


Registered: 01/17/24
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte] 1
#28632221 - 01/23/24 03:22 PM (4 days, 11 hours ago) |
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Thank you Pastywhyte for this monumental thread. I've been digesting it a little at a time for the past week and I'm on page 263 lol. I just can't believe how awesome the community is with several fine folks repeatedly answering the same questions to help new aspiring mycologists get started. You guys fucking rock.
I've got my first couple batches of pasty plates noc'd up, I'm not worried about condensation, I'm excited to be doing agar instead of the PF tek I did back in 2000, and I can't wait to enjoy the fruits of these labors!
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Pastywhyte's Easy Agar Tek [Re: Thankful] 1
#28637500 - 01/27/24 11:33 PM (3 hours, 10 minutes ago) |
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Wow I can’t believe it’s been 10 years since I posted this. Heady times. Thanks to everyone who’s bumped and contributed to it over the years. You are all a part of what makes this place great.
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