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 x_bonerz_x
Du Ma May ConCac Lon!
Registered: 01/21/03
Posts: 163
Last seen: 14 years, 11 months
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 Test Tube + Agar Q 
#1331535 - 02/24/03 12:54 PM (15 years, 2 months ago) |
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Instead of using the actual piece of mushroom, are we able to do this with just Mycelium??
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KingCobWeb
journeyman
Registered: 11/20/02
Posts: 97
Last seen: 14 years, 11 months
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Re: Test Tube + Agar Q [Re: x_bonerz_x]
#1332572 - 02/24/03 07:52 PM (15 years, 2 months ago) |
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Quote:
Instead of using the actual piece of mushroom, are we able to do this with just Mycelium??
Yes.
My understanding is that the mushroom IS basically mycelium, and it will grow more mycelium when placed on or in a nutrient agar plate (Or testtube, if you will)
Regular old mycelium will grow the same way, maybe even a little quicker...and maybe a bit cleaner (less contams) depending on where the mycelioum came from.
When using a grown mushroom (Dont use aborts, use a healthy shroom before the viel breaks) it is probably more prone to contams just because once a person plucks the shroom, and does the happy "Looky looky! I just grew and picked a shroom!" dance all about the room, the shroom is then covered in all kinds of spores and stuff.
A dunk in some H2O2/water solution will help take off some of the contams before carefully disecting the shroom and plopping a peice of it on the agar.
It is VERY important that while singing to your mycelium or shrooms during this phase that you are careful NOT to sing directly onto the shroom or agar or mycelium...the breath exhaled will contain contams. Wear a dust mask while singing to your shrooms or samples, or while singing around agar.
Oh, and use the material from INSIDE the shroom after "peeling" it under very sterile conditions. (Dont sing directly to it) There is a tek for making shroom water using a shroom for innoculating, read that for a good idea of how to get a good peice of mycelium from a mushroom.
And think positively!
If Bush doesnt get us all killed, the rest of the world will kill us!
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