Home | Community | Message Board


SoulSpeciosa Kratom
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, Agar, Brown Rice Flour, Petri Dish

Jump to first unread post. Pages: 1
OfflineUnknown
Confused,dizzy,wheream I again?
Registered: 01/07/02
Posts: 380
Loc: MA
Last seen: 14 years, 1 month
Sterilising Agar
    #1427546 - 04/04/03 02:21 AM (15 years, 1 month ago)

Can you skip PC'ing the agar? Since it's boiling when you mix it can't you just pour it directly to the plates? Or let it harden in a sealed jar,then heat the jar in a pan of water until it reliquifies?


--------------------
The above is just like,my opinion man


Edited by Paradis (04/04/03 02:33 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblewoodrow
journeyman
Registered: 03/17/03
Posts: 138
Re: Sterilising Agar [Re: Anonymous]
    #1427734 - 04/04/03 03:52 AM (15 years, 1 month ago)

Boiling agar does not get it hot enough to kill the bacterial spores. You still need to PC it.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineUnknown
Confused,dizzy,wheream I again?
Registered: 01/07/02
Posts: 380
Loc: MA
Last seen: 14 years, 1 month
Re: Sterilising Agar [Re: woodrow]
    #1427753 - 04/04/03 04:04 AM (15 years, 1 month ago)

Okay,damn.


--------------------
The above is just like,my opinion man


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineBaby_Hitler
Errorist
 User Gallery

Folding@home Statistics
Registered: 03/06/02
Posts: 23,091
Loc: To the limit!
Last seen: 3 hours, 15 minutes
Re: Sterilising Agar [Re: Anonymous]
    #1428193 - 04/04/03 09:02 AM (15 years, 1 month ago)

I use peroxidated agar without a PC. I boil the water add the agar and stuff, mix it, then put it in a pot of boiling water with a lid on it for an hour.

You can't germinate spores on peroxidated agar, but you can germinate in BRF and cut a piece out and put it on the agar.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Sterilising Agar [Re: Anonymous]
    #1428366 - 04/04/03 10:55 AM (15 years, 1 month ago)

You must sterilize it .
You can reheat to liquify again then pour , but is best to make and use what you have planned in one sitting . But make sure it is kept sealed and cool until you use it , and use ASAP .
PC or AC for 30-40 min , let cool till you can hold jar in bare hand , pour into petris , let solidify , then inoc with spores . IMO , is best to let dishes sit around in dark , clean place for one to two days before inocing . That way you know you have uncontaminated dishes of agar prior to inocing with spores .
Store unused agar in fridge , when want to use again put into a pot of hot water for 20-30 min or until liquid that can be shaken up to remix , then pour as usuall .
Good Luck


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars, Agar, Brown Rice Flour, Petri Dish

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Agar Agar peroxide jellyfish 1,117 5 07/20/06 11:59 PM
by fastfred
* Agar & Peroxide Question oddiseus 1,000 7 02/22/05 06:25 PM
by oddiseus
* agar/peroxide conc. iudexk 619 1 08/27/01 03:17 PM
by jonnyshaggs420
* Re: Agar w/ peroxide info Hippie3 772 2 05/07/00 04:20 AM
by mycofile
* Re: Antibiotic Agar Mix for Spore Germiniation in Water? Anonymous 651 1 05/09/00 08:46 PM
by Anonymous
* buying agar - help sinisha 1,859 6 03/31/06 07:49 AM
by FractalXplora
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 2,605 8 02/14/05 09:57 AM
by Roadkill
* Potato Dextrose Agar X Malt Extract Agar Alegalize 2,098 8 02/25/05 01:03 PM
by agar

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
654 topic views. 23 members, 88 guests and 10 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Mycohaus
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.005 seconds on 19 queries.