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OfflineUnknown
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Sterilising Agar
    #1427546 - 04/04/03 02:21 AM (13 years, 8 months ago)

Can you skip PC'ing the agar? Since it's boiling when you mix it can't you just pour it directly to the plates? Or let it harden in a sealed jar,then heat the jar in a pan of water until it reliquifies?


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The above is just like,my opinion man


Edited by Paradis (04/04/03 02:33 AM)


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Invisiblewoodrow
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Registered: 03/17/03
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Re: Sterilising Agar [Re: Anonymous]
    #1427734 - 04/04/03 03:52 AM (13 years, 8 months ago)

Boiling agar does not get it hot enough to kill the bacterial spores. You still need to PC it.


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OfflineUnknown
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Re: Sterilising Agar [Re: woodrow]
    #1427753 - 04/04/03 04:04 AM (13 years, 8 months ago)

Okay,damn.


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The above is just like,my opinion man


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OfflineBaby_Hitler
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Re: Sterilising Agar [Re: Anonymous]
    #1428193 - 04/04/03 09:02 AM (13 years, 8 months ago)

I use peroxidated agar without a PC. I boil the water add the agar and stuff, mix it, then put it in a pot of boiling water with a lid on it for an hour.

You can't germinate spores on peroxidated agar, but you can germinate in BRF and cut a piece out and put it on the agar.


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Invisibletripndicular
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Re: Sterilising Agar [Re: Anonymous]
    #1428366 - 04/04/03 10:55 AM (13 years, 8 months ago)

You must sterilize it .
You can reheat to liquify again then pour , but is best to make and use what you have planned in one sitting . But make sure it is kept sealed and cool until you use it , and use ASAP .
PC or AC for 30-40 min , let cool till you can hold jar in bare hand , pour into petris , let solidify , then inoc with spores . IMO , is best to let dishes sit around in dark , clean place for one to two days before inocing . That way you know you have uncontaminated dishes of agar prior to inocing with spores .
Store unused agar in fridge , when want to use again put into a pot of hot water for 20-30 min or until liquid that can be shaken up to remix , then pour as usuall .
Good Luck


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Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Petri Dish

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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