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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: What to do with these grain spawns [Re: Mr. Funguy]
    #25858414 -

Now I don't even understand what ur talkimg about... throwing out contaminated spawn is in no way "my way".. It's THE way. The only way.

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Re: What to do with these grain spawns [Re: nube424]
    #25858425 -

Saying the OP thinks things can be done differently because he has never done them, so something that is new is always able to be changed by not knowing what to correctly do.
By knowing what to CORRECTLY do, there wont be any mistakes, purposely.
By saying "no dont do this", "well why not, I'm gonna do it any way".
If you say, "dont keep it because of this and this and this or that", it's easier to understand. Like the link I shared, it takes time for things to click for some people.


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Re: What to do with these grain spawns [Re: Mr. Funguy]
    #25858430 -

I told him several times not to keep it because it's mold. Look at the first page. That shouldn't need any further explaining. The explanation was "it's brown mold"

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Re: What to do with these grain spawns [Re: Shroombie2]
    #25858503 -

BTW, does this mean that one shouldn't mix spores with different strains in a jar? Will this always be the outcome with grains?

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Re: What to do with these grain spawns [Re: Shroombie2]
    #25858515 -

This has nothing to do with using different varieties of spores in the same jar. It's because there were spores of some species of mold that mixed in.


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How I do glass dishes

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Re: What to do with these grain spawns [Re: nube424]
    #25858520 -

I said it's brown I have not seen contaminates like this. Well now I have thanks to the pictures Mad Season posted. The posts suggests the mold is because of mix of species. It just felt like something I could perhaps get away with in a separate container for some fruits so I kept asking because I'm out of mushrooms and I was hoping there was a way to get some fruits out of this. I'm sorry if that upset you, I didn't know it would. I have listened to your advice now and thrown the jar out and as expressed before, I appreciate your help and input.

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Re: What to do with these grain spawns [Re: poisoned]
    #25858524 -

Aha okay, I thought "fungal species" meant mushroom species.

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Re: What to do with these grain spawns [Re: Shroombie2]
    #25858526 -

No. U got brown mold because there was grown mold spores either in ur syringe or u didn't properly sterilize the grain and it was already in there. The only reason u would ever want to mix 2 different syringes is if ur trying to make a cross.. but u gotta learn the basics before u attempt crossing mushroom varieties.

Why did u mix them tho? U only need a few drops of spore solution for a grain jar. Adding more doesn't help, it's actually counter productive. Ur adding extra water that doesn't need to be there, and most/all syringes are somewhat dirty. So adding more solution only ups ur chances of adding contamination.

Did u prep these jars urself or were they pre made? If u made them, how long did u sterilize them.

Better funguy?

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Re: What to do with these grain spawns [Re: nube424]
    #25858531 -

Much...

Edit-
I learned something, I was gonna put KSSS and APE from spore in a jar and see what happens. Now, straight to agar, fuck it...


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Edited by Mr. Funguy (03/07/19 01:59 PM)

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Re: What to do with these grain spawns [Re: nube424]
    #25858543 -

90 minutes after reaching 1.5 PSI and my manifacturer guarantees me (Don't get all mad again now) that these syringes are contamination free and I sterilize the needle with a torch lighter before each jar. That's also a reason to why I didn't want to think I have done something wrong. The only thing I recall having done that wasn't in the instructions was that I injected a lot of spores.

I explained in the first post that I did a stupid thing and injected 20ml in 4 jars which I now understand was stupid.

Edited by Shroombie2 (03/07/19 02:06 PM)

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Re: What to do with these grain spawns [Re: Shroombie2]
    #25858549 -

Eh not really. If you do 4ml in four ports it's ok, 1ml per port. If you do 4ml in one, it is a waste, not really what would cause bacteria.
If the producer of the spores guarantees them clean, try pcing grain longer AFTER a good soak then boil. If that doesnt work, tell them their guarantee was off on that batch. If they say no, fuck it try somewhere else. If you are low on money, wait for you 90 days to be up and go to marketplace and grab a giveaway print.

Edit- 5ml*


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Re: What to do with these grain spawns [Re: Shroombie2]
    #25858572 -

I have already like 7-8 other syringes I have bought but I'm planning to not buy one again if it's not for trying a new specie. I have bought the agar supply I need just need to do it for the first time soon. I think I did 20 ml on total of 8 ports, 2 on each. One jar didn't even get colonized. Also I didn't shake the jars even once until now during colonization, didn't know about it.

I have done the same process with 4 new jars but this time just inoculated with just some drops. These jars looks much better and colonizes faster but it could also be that I got the moisture in the jars right the second time. One jar didn't inoculate the second time, it was exactly the same jar that didn't inoculate the first time. I just resterilized it and put some more spores in it and hoped it would colonize but instead it was really really dry.

I have also recently inoculated 2 flat 1200 ml containers with rye. I'm thinking perhaps it could colonize faster because I could just drop spores all over it and inside the corners. I will find out in 2 weeks.

Edited by Shroombie2 (03/07/19 02:23 PM)

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Re: What to do with these grain spawns [Re: Mr. Funguy]
    #25858578 -

Syringes are never clean so they lied. 5ml is way too much. 1 ml per quart is even alot. What did u use for gas exchange on those lids?

And I would recommend getting some agar supplies. Using syringes with grain is a bad idea for this very reason.

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Re: What to do with these grain spawns [Re: nube424]
    #25858586 -

The container lids has filters that are designed for this purpose. They keep moisture and allow GE. I used to pierce the syringe needle through the lid and that would cause 2 new very small holes. I don't do that anymore, I simply take off the lid for a second to inject the spores and then put them back on. It's works and it's easier and faster and I can reuse the lids.

Edited by Shroombie2 (03/07/19 02:29 PM)

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Re: What to do with these grain spawns [Re: Shroombie2]
    #25858589 -

If you are doing all of this in a clean and sterile environment, fuck ya, more power to ya, if you're doing it in open air, it's already gonna go bad. there are microscopic bacteria floating everywhere. A SAB (Still Air Box) or Flow hood is the only ideal way to do anything you're talking about. except the bin, it's always better to contain it til fully colonized, than to do it open air, unless it's in a clean and sterile environment, then fuck ya upload pics...


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Re: What to do with these grain spawns [Re: nube424]
    #25858595 -

What do u mean flat containers?

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Re: What to do with these grain spawns [Re: Mr. Funguy]
    #25858597 -

I'm doing it in open air in my kitchen. But I was more careful the first times.

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Re: What to do with these grain spawns [Re: nube424]
    #25858600 -

https://imgur.com/ic0Ff3U
https://imgur.com/juYdAly

I let them in PC for an hour on 15 PSI, let them cool, opened the lids and injected spores on the corners and some drops on top and put the lids back on this Monday. I was a bit careless this time with the PC time so it will be interesting to see if I get contamination.

Edited by Shroombie2 (03/07/19 02:38 PM)

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Re: What to do with these grain spawns [Re: nube424]
    #25858602 -

This dude needs some teks to follow. I'll find u some links broski. The way ur doing things are completely wrong. Where did u get all ur info from? It couldn't have been this site...

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Re: What to do with these grain spawns [Re: Shroombie2]
    #25858605 -

Shroombie2 said:
I'm doing it in open air in my kitchen. But I was more careful the first times.


U can't open jars in open air my man

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