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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Spores to agar +6weeks 1st growth is contam
    #25556703 -

After six weeks the spores germinated. But then it germinated contamination.

I want to know if that sounds right. Or did I have a vector somewhere?

Backstory
I have a spore print, roughly a year and half old, foolishly did not refrigerate. I put it on agar and waited. There was no contam visible until I saw on the 6th week some growth. So I transferred and found that it was green mold. How is that possible.

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Re: Spores to agar +6weeks 1st growth is contam [Re: District]
    #25556704 -

This growth came from various spore sites and appeared roughly at the same time.

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Re: Spores to agar +6weeks 1st growth is contam [Re: District]
    #25556874 -

Sounds as though the environment wasn't right for growth until week 6? Temp maybe?


--------------------
"How it should & shouldn't look "      "Recognizing contamination"      "Rye Berry Prep"      "Franks G2G"      "Humidity Chamber"      "Agar Recipes"

"How to dial in your monotubs like a Boss"      "Pastywhyte's Easy Agar Tek"      "Elementary [Damion5050]Coir Tek"        "SGFC"      "SAB Tek"      "Easy AF Shoeboxes"


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Re: Spores to agar +6weeks 1st growth is contam [Re: Shroomspective]
    #25559657 -

Its the agar formulation. But what does that have to do with contam.

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Re: Spores to agar +6weeks 1st growth is contam [Re: District]
    #25559659 -

I got growth after 6 weeks and its contam from various spore sites, which confuses me.

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Re: Spores to agar +6weeks 1st growth is contam [Re: Shroomspective]
    #25559660 -

Its the agar formulation. But what does that have to do with contam.

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Re: Spores to agar +6weeks 1st growth is contam [Re: District]
    #25559665 -

If it were the medium mixture I can't see why that the cotam spores would take 6 weeks to germinate..It would have to be moisture or temperature levels I should think, the nutrients would then affect how the spores grew..Either way I should think either your spore samples aren't clean, the trich spores must be mixed in with the prints, or introduced at inoculation


--------------------
"How it should & shouldn't look "      "Recognizing contamination"      "Rye Berry Prep"      "Franks G2G"      "Humidity Chamber"      "Agar Recipes"

"How to dial in your monotubs like a Boss"      "Pastywhyte's Easy Agar Tek"      "Elementary [Damion5050]Coir Tek"        "SGFC"      "SAB Tek"      "Easy AF Shoeboxes"


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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