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Invisiblerepobob
enthusiast
Registered: 12/19/01
Posts: 261
Loc: Illinois
Karo & Neutritional Yeast Contam?????
    #1120071 - 12/07/02 04:42 PM (13 years, 11 months ago)

I innoculated several jars of Kayro + NY a few days ago. All are looking good, except one. A quart jar I shot with PF Classic mycilium from another Kayro without NY jar ( I'm stoned, Hope you undestand what I just said. :confused:)
Anyway this quart had some wispy mycilium growing on the bottom as it shoud, but it also has little pure white spots the size of a rice grain clinging to the side of the jar and floating at the top. Sure looks like mycilium to me, but i have never seen it float like this. Any help???

Thanks
Bob 


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Edited by repobob (12/07/02 04:46 PM)


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Invisiblezeta
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Registered: 05/25/02
Posts: 3,972
Re: Karo & Neutritional Yeast Contam????? [Re: repobob]
    #1120269 - 12/07/02 06:58 PM (13 years, 11 months ago)

How did you sterilise?


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Invisiblerepobob
enthusiast
Registered: 12/19/01
Posts: 261
Loc: Illinois
Re: Karo & Neutritional Yeast Contam????? [Re: zeta]
    #1120281 - 12/07/02 07:18 PM (13 years, 11 months ago)

I PC'd at 10lbs for 15 min. I did 7 jars in that batch and only one is growing in this fashion. If I got contam it had to be when I shot the up.
:confused: :confused: :confused:

Peace,
Bob


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OfflineMAIA
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Re: Karo & Neutritional Yeast Contam????? [Re: repobob]
    #1121663 - 12/08/02 08:00 AM (13 years, 11 months ago)

Maybe. But man, 15 min. at 10lbs is too short for a good sterilization.

MAIA


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Invisiblerepobob
enthusiast
Registered: 12/19/01
Posts: 261
Loc: Illinois
Re: Karo & Neutritional Yeast Contam????? [Re: MAIA]
    #1121731 - 12/08/02 10:25 AM (13 years, 11 months ago)

You think so????
I figured I was sterilizing a liquid which conducts heat easier than grain it would be shorter. In other words it takes longer for heat to warm the center of a Quart of Birdseed than to heat a Quart of liquid. Besides if this stuff gets to hot it carmelizes then you got shit. Again only one jar of seven has this growth so I really don't think its in the PC'ing. If the sterilization was bad i would think more than one jar would be contaminated. Oh well, I'm confused too. :confused:

As a side note the jars that have growth are tremendous compared to the honey or kayro alone I did before. In less than four days I have a mass 3/4 inch thick across the entire bottom of the jars. It is white and very wispy. It gets thicker as time goes on. So far this Neutritional Yeast looks really good. We'll see how the rest of the growing cycle goes with it.

Peace,
Bob 


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OfflineStonedhenge
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Registered: 10/05/02
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Re: Karo & Neutritional Yeast Contam????? [Re: repobob]
    #1121978 - 12/08/02 01:55 PM (13 years, 11 months ago)

try a search for karo tek some say p/cing too long causes it to carmelize. i havent tried so i cant say for sure good luck


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Invisiblerepobob
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Registered: 12/19/01
Posts: 261
Loc: Illinois
Re: Karo & Neutritional Yeast Contam????? [Re: Stonedhenge]
    #1122067 - 12/08/02 02:36 PM (13 years, 11 months ago)

"Besides if this stuff gets to hot it carmelizes then you got shit."
Yes you are correct, but thats pretty much what I said in my post. But, hey thanks for concuring with me :smile:
Also I have done this tek several times as stated in my post so could you please explain to me what I will gain by doing a search on the Karo Tek. But hey, thanks again :smile:
Thanks for trying to help me
You should try this tek Stoned, it works well, and thanks again for the search advice, I never would have thought of that. :smile: :smirk: :smile: :smirk:
Can anyone help with this. 


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Edited by repobob (12/08/02 02:37 PM)


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Anonymous

Re: Karo & Neutritional Yeast Contam????? [Re: repobob]
    #1122736 - 12/08/02 09:20 PM (13 years, 11 months ago)

I'm a big fan of using Karo water. What ratio of nutritional yeast to karo syrup to water did you use? This sounds like a promising prospect.


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Invisiblerepobob
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Registered: 12/19/01
Posts: 261
Loc: Illinois
Re: Karo & Neutritional Yeast Contam????? [Re: ]
    #1124070 - 12/09/02 08:17 AM (13 years, 11 months ago)

I got this from http://www.nansnook.com/
By Visions (Visions) on Thursday, May 23, 2002 - 05:31 pm: Edit

Hello everyone....I wanted to show you one of my experiments I have going...Let
me show you what happens when you take the Karo Tek and add Nutritional Yeast.

Nutritional Yeast


Both Jars are Qt. and have 2tbls of Karo. Both were inoculated with 1ml of P.F.
spores from the same syringe and keep around 80deg for 7 days. The only difference is the addition of 1/4tsp of Nutritional Yeast to one of the jars.

There is one
drawback and that is Nutritional Yeast adds a carmel color. I have done this
before and the longer you let the mycelium grow the more clear the water gets.

P.F. & Karo side view
Sorry pictures didn't copy, but they are at Nan'sNook.

P.F. & Karo bottom view


P.F. & Karo & Nutritional Yeast side view


P.F. & Karo & Nutritional Yeast bottom view


P.F. & Karo with and without Nutritional Yeast


The pictures don't do this justice. The
mycelium from the regular Karo jar has a very whispy cloud formation. The Karo jar
with the Nutritional Yeast has a very robust and dense mycelium and as you can
see must be 20-40 times larger. This opens up some more options like adding the
Nutritional Yeast to every substrate.


Some people might worry about contams because the solution isn't clear but for
me I have never had a problem with liquid culture or contaminated jars. The
contams for me come in after you open the jars and after you birth the cake.
Most likely the contams would be intoduced from repeated withdraws into the
syinges and not being clean.

While looking at the Nutritional breakdown
of this Yeast supplement I noticed that about 50% of the calories are from
Protien this may account for how Large, Dense and Strong the mycelium looks.
Whatever the reason the mycelium loves it!

My next experiment will be to see if
the entire growth cycle, all the way to fruiting, can be done only using
Nutritional Yeast and Verm. I really don't think the Karo is even needed in this
liquid culture. Well let me take that back...There couldn't be that much growth
on just .25tsp of Nutritional Yeast @ only 15 calories.

Anyone else willing to
experiment with this stuff would sure help to confirm or disprove my results.


Well, what do you think?


NUTRITIONAL YEAST BREAKDOWN
Serving size 1 heaping Tablespoon
Calories = 60
Calories from fat = 10
Total Carbohidrate = 6g
Protein = 7g
Vitamin A = 0
Vitamin C = 0
Thiamin = 8mg
Riboflavin = 8mg
Niacin = 40mg
Vitamin B6 = 8mg
Folic Acid = 100mcg
Vitamin B12 = 12mcg
Biotin = 25mcg
Pantothenic acid = 30mg
Calcium = 500mg
Iron = 5mg
Phosphorus = 400mg
Iodine = 100mcg
Magnesium = 150mg
Zinc = 2mg
Copper = 280mcg
Manganese = 500mcg
Chromium = 30mcg
Sodium = 0
Potassium = 320mg
In a base containing 30mg Para-aminobenzoic acid, 55mg Choline and 64mg Inositol

By Maliki (Maliki) on Thursday, May 23, 2002 - 05:44 pm: Edit


Good deal man glad to see you got the pictures up.
What was your solution ? to the error????
Not to meention the yeast idea seems like a very solid one.......

By Visions (Visions) on Thursday, May 23, 2002 - 06:13 pm: Edit


Thanks Maliki....I did a dianostic on IE and it had corrupted files...

Everyday the mycelium keeps getting noticeably bigger!

I am afraid if I take the lid off it might crawl out and get me!!!

About 8-10 months ago I grew mycelium in 3gal jugs that you make beer in with just Dextrose and it had mediocre growth so not being happy with it I opened it and dumped in some Nutritional Yeast...Well it went crazy...but when I did it I wasn't very careful and it contamed...and I had many experiments going on and sort of lost track of the idea...but here it is again! For those people that don't want to grow the accual mushroom and just the mycelium to harvest and dry this would be the way to go. And for just one easy payment of $29.95 I will show you how to do it step by step!!! LOL...Just Kidding

By Visions (Visions) on Thursday, May 23, 2002 - 06:37 pm: Edit


I have one more note: After I inject the spores I don't move the jars, I don't shake or stir them. I let all the spores mate on the bottom and connect and grow into one large mycelium. Now when it comes time to fill syinges I will shake the living hell out of it! I think they become stronger letting them grow together. And they want to do it...why would I try to stop mother nature...she has her reasons!

By Dr. Cubesis III (Newbieshroomer) on Thursday, May 23, 2002 - 07:09 pm: Edit


Nice visions

Tell ya tho... A lot of people take one look at all of that text you posted and run. LOL! I should know

By Visions (Visions) on Thursday, May 23, 2002 - 08:17 pm: Edit


Dr. you're right....I do the same thing....and before I posted it I looked at it and thought--Damn that's long! LOL...I should have broken it up so it would be easier to read....But I think the pictures will make them put their reading glasses on.....if they can make it to the pics!

By Visions (Visions) on Thursday, May 23, 2002 - 08:52 pm: Edit


Hey Dr. as you can see I took that Novel I wrote and broke it up with Pretty Pictures...

By Dr. Cubesis III (Newbieshroomer) on Thursday, May 23, 2002 - 09:01 pm: Edit


YES!!

Breaking it up with the pretty pictures did the trick for me!! If they can make it to the pix?!!?
LOLOLOL!!! Visions! Have a great day!!

By Hippie3 (Admin) on Thursday, May 23, 2002 - 10:17 pm: Edit


you should enter this in our contest visions while there is still time.


By onediadem (Onediadem) on Friday, May 24, 2002 - 01:29 pm: Edit


Hey Zilla, keep hearing about those pics, lol, lookin forward to you posting them too.

By cowshitblues (Cowshitblues) on Friday, May 24, 2002 - 04:05 pm: Edit


have you used any to inocculate a jar???.. any differences... that looks awsome... have some karo water ... might try a few with N.Y...the ingredients look awsome for growth...

By cowshitblues (Cowshitblues) on Friday, May 24, 2002 - 06:05 pm: Edit


sorry bout the 2nd post.. but i noticed iodine in there... hmmm sounds like a good anti-bacterial too....sweetness... my friend is gonna add add some to a few brf substrates...in different amounts.. i will post his findings for him findings when done...also my friend is gonna try a brf powdered cake spawned into wormcasing+yeast....

By Imok Urok2 (Imok) on Saturday, May 25, 2002 - 02:33 am: Edit


Hey visions,
If you want this to be moved to the contest thread just say the word.
Love the pics and the tek.
First, hippie invitro with chronic twist.
Then, cold shock/bleach dunk tek.
Now this.
We live in wonderful times folks


By bill cogwell (Iphishphan) on Saturday, May 25, 2002 - 02:51 am: Edit


how much of the solution should you use to inoculate i have a few syringes i did karo style and some of them dont have as much mycelium in them as the others. Are their microscopic mycelium i dont see?

By xylem flow (Xylem) on Saturday, May 25, 2002 - 03:19 am: Edit


good question bill

i wanna know too.

By Hippie3 (Admin) on Wednesday, September 11, 2002 - 02:41 am: Edit


i just made a qt with 2 tsp of nut. yeast, we'll see what grows.

By Admin (Admin) on Tuesday, November 19, 2002 - 01:09 pm: Edit


here's mine-


Chronic007


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