|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
repobob
enthusiast
Registered: 12/18/01
Posts: 261
Loc: Illinois
|
Karo & Neutritional Yeast Contam?????
#1120071 - 12/07/02 02:42 PM (22 years, 2 months ago) |
|
|
I innoculated several jars of Kayro + NY a few days ago. All are looking good, except one. A quart jar I shot with PF Classic mycilium from another Kayro without NY jar ( I'm stoned, Hope you undestand what I just said. ) Anyway this quart had some wispy mycilium growing on the bottom as it shoud, but it also has little pure white spots the size of a rice grain clinging to the side of the jar and floating at the top. Sure looks like mycilium to me, but i have never seen it float like this. Any help???
Thanks Bob
-------------------- .
Edited by repobob (12/07/02 02:46 PM)
|
zeta
Stranger

Registered: 05/24/02
Posts: 3,972
|
Re: Karo & Neutritional Yeast Contam????? [Re: repobob]
#1120269 - 12/07/02 04:58 PM (22 years, 2 months ago) |
|
|
How did you sterilise?
|
repobob
enthusiast
Registered: 12/18/01
Posts: 261
Loc: Illinois
|
Re: Karo & Neutritional Yeast Contam????? [Re: zeta]
#1120281 - 12/07/02 05:18 PM (22 years, 2 months ago) |
|
|
I PC'd at 10lbs for 15 min. I did 7 jars in that batch and only one is growing in this fashion. If I got contam it had to be when I shot the up. 
Peace, Bob
-------------------- .
|
MAIA
World-BridgerKartikeya (DftS)


Registered: 04/27/01
Posts: 7,399
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 28 days, 1 hour
|
Re: Karo & Neutritional Yeast Contam????? [Re: repobob]
#1121663 - 12/08/02 06:00 AM (22 years, 2 months ago) |
|
|
Maybe. But man, 15 min. at 10lbs is too short for a good sterilization.
MAIA
-------------------- Spiritual being, living a human experience ... The Shroomery Mandala

Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
|
repobob
enthusiast
Registered: 12/18/01
Posts: 261
Loc: Illinois
|
Re: Karo & Neutritional Yeast Contam????? [Re: MAIA]
#1121731 - 12/08/02 08:25 AM (22 years, 2 months ago) |
|
|
You think so???? I figured I was sterilizing a liquid which conducts heat easier than grain it would be shorter. In other words it takes longer for heat to warm the center of a Quart of Birdseed than to heat a Quart of liquid. Besides if this stuff gets to hot it carmelizes then you got shit. Again only one jar of seven has this growth so I really don't think its in the PC'ing. If the sterilization was bad i would think more than one jar would be contaminated. Oh well, I'm confused too. 
As a side note the jars that have growth are tremendous compared to the honey or kayro alone I did before. In less than four days I have a mass 3/4 inch thick across the entire bottom of the jars. It is white and very wispy. It gets thicker as time goes on. So far this Neutritional Yeast looks really good. We'll see how the rest of the growing cycle goes with it.
Peace, Bob
-------------------- .
|
Stonedhenge
searcher

Registered: 10/05/02
Posts: 27
Last seen: 21 years, 8 months
|
Re: Karo & Neutritional Yeast Contam????? [Re: repobob]
#1121978 - 12/08/02 11:55 AM (22 years, 2 months ago) |
|
|
try a search for karo tek some say p/cing too long causes it to carmelize. i havent tried so i cant say for sure good luck
-------------------- good for nothing's good enough for me!
|
repobob
enthusiast
Registered: 12/18/01
Posts: 261
Loc: Illinois
|
Re: Karo & Neutritional Yeast Contam????? [Re: Stonedhenge]
#1122067 - 12/08/02 12:36 PM (22 years, 2 months ago) |
|
|
"Besides if this stuff gets to hot it carmelizes then you got shit." Yes you are correct, but thats pretty much what I said in my post. But, hey thanks for concuring with me  Also I have done this tek several times as stated in my post so could you please explain to me what I will gain by doing a search on the Karo Tek. But hey, thanks again  Thanks for trying to help me You should try this tek Stoned, it works well, and thanks again for the search advice, I never would have thought of that.  Can anyone help with this.
-------------------- .
Edited by repobob (12/08/02 12:37 PM)
|
Anonymous
|
Re: Karo & Neutritional Yeast Contam????? [Re: repobob]
#1122736 - 12/08/02 07:20 PM (22 years, 2 months ago) |
|
|
I'm a big fan of using Karo water. What ratio of nutritional yeast to karo syrup to water did you use? This sounds like a promising prospect.
|
repobob
enthusiast
Registered: 12/18/01
Posts: 261
Loc: Illinois
|
Re: Karo & Neutritional Yeast Contam????? [Re: ]
#1124070 - 12/09/02 06:17 AM (22 years, 2 months ago) |
|
|
I got this from http://www.nansnook.com/ By Visions (Visions) on Thursday, May 23, 2002 - 05:31 pm: Edit
Hello everyone....I wanted to show you one of my experiments I have going...Let me show you what happens when you take the Karo Tek and add Nutritional Yeast.
Nutritional Yeast
Both Jars are Qt. and have 2tbls of Karo. Both were inoculated with 1ml of P.F. spores from the same syringe and keep around 80deg for 7 days. The only difference is the addition of 1/4tsp of Nutritional Yeast to one of the jars.
There is one drawback and that is Nutritional Yeast adds a carmel color. I have done this before and the longer you let the mycelium grow the more clear the water gets.
P.F. & Karo side view Sorry pictures didn't copy, but they are at Nan'sNook.
P.F. & Karo bottom view
P.F. & Karo & Nutritional Yeast side view
P.F. & Karo & Nutritional Yeast bottom view
P.F. & Karo with and without Nutritional Yeast
The pictures don't do this justice. The mycelium from the regular Karo jar has a very whispy cloud formation. The Karo jar with the Nutritional Yeast has a very robust and dense mycelium and as you can see must be 20-40 times larger. This opens up some more options like adding the Nutritional Yeast to every substrate.
Some people might worry about contams because the solution isn't clear but for me I have never had a problem with liquid culture or contaminated jars. The contams for me come in after you open the jars and after you birth the cake. Most likely the contams would be intoduced from repeated withdraws into the syinges and not being clean.
While looking at the Nutritional breakdown of this Yeast supplement I noticed that about 50% of the calories are from Protien this may account for how Large, Dense and Strong the mycelium looks. Whatever the reason the mycelium loves it!
My next experiment will be to see if the entire growth cycle, all the way to fruiting, can be done only using Nutritional Yeast and Verm. I really don't think the Karo is even needed in this liquid culture. Well let me take that back...There couldn't be that much growth on just .25tsp of Nutritional Yeast @ only 15 calories.
Anyone else willing to experiment with this stuff would sure help to confirm or disprove my results.
Well, what do you think?
NUTRITIONAL YEAST BREAKDOWN Serving size 1 heaping Tablespoon Calories = 60 Calories from fat = 10 Total Carbohidrate = 6g Protein = 7g Vitamin A = 0 Vitamin C = 0 Thiamin = 8mg Riboflavin = 8mg Niacin = 40mg Vitamin B6 = 8mg Folic Acid = 100mcg Vitamin B12 = 12mcg Biotin = 25mcg Pantothenic acid = 30mg Calcium = 500mg Iron = 5mg Phosphorus = 400mg Iodine = 100mcg Magnesium = 150mg Zinc = 2mg Copper = 280mcg Manganese = 500mcg Chromium = 30mcg Sodium = 0 Potassium = 320mg In a base containing 30mg Para-aminobenzoic acid, 55mg Choline and 64mg Inositol
By Maliki (Maliki) on Thursday, May 23, 2002 - 05:44 pm: Edit
Good deal man glad to see you got the pictures up. What was your solution ? to the error???? Not to meention the yeast idea seems like a very solid one.......
By Visions (Visions) on Thursday, May 23, 2002 - 06:13 pm: Edit
Thanks Maliki....I did a dianostic on IE and it had corrupted files...
Everyday the mycelium keeps getting noticeably bigger!
I am afraid if I take the lid off it might crawl out and get me!!!
About 8-10 months ago I grew mycelium in 3gal jugs that you make beer in with just Dextrose and it had mediocre growth so not being happy with it I opened it and dumped in some Nutritional Yeast...Well it went crazy...but when I did it I wasn't very careful and it contamed...and I had many experiments going on and sort of lost track of the idea...but here it is again! For those people that don't want to grow the accual mushroom and just the mycelium to harvest and dry this would be the way to go. And for just one easy payment of $29.95 I will show you how to do it step by step!!! LOL...Just Kidding
By Visions (Visions) on Thursday, May 23, 2002 - 06:37 pm: Edit
I have one more note: After I inject the spores I don't move the jars, I don't shake or stir them. I let all the spores mate on the bottom and connect and grow into one large mycelium. Now when it comes time to fill syinges I will shake the living hell out of it! I think they become stronger letting them grow together. And they want to do it...why would I try to stop mother nature...she has her reasons!
By Dr. Cubesis III (Newbieshroomer) on Thursday, May 23, 2002 - 07:09 pm: Edit
Nice visions
Tell ya tho... A lot of people take one look at all of that text you posted and run. LOL! I should know
By Visions (Visions) on Thursday, May 23, 2002 - 08:17 pm: Edit
Dr. you're right....I do the same thing....and before I posted it I looked at it and thought--Damn that's long! LOL...I should have broken it up so it would be easier to read....But I think the pictures will make them put their reading glasses on.....if they can make it to the pics!
By Visions (Visions) on Thursday, May 23, 2002 - 08:52 pm: Edit
Hey Dr. as you can see I took that Novel I wrote and broke it up with Pretty Pictures...
By Dr. Cubesis III (Newbieshroomer) on Thursday, May 23, 2002 - 09:01 pm: Edit
YES!!
Breaking it up with the pretty pictures did the trick for me!! If they can make it to the pix?!!? LOLOLOL!!! Visions! Have a great day!!
By Hippie3 (Admin) on Thursday, May 23, 2002 - 10:17 pm: Edit
you should enter this in our contest visions while there is still time.
By onediadem (Onediadem) on Friday, May 24, 2002 - 01:29 pm: Edit
Hey Zilla, keep hearing about those pics, lol, lookin forward to you posting them too.
By cowshitblues (Cowshitblues) on Friday, May 24, 2002 - 04:05 pm: Edit
have you used any to inocculate a jar???.. any differences... that looks awsome... have some karo water ... might try a few with N.Y...the ingredients look awsome for growth...
By cowshitblues (Cowshitblues) on Friday, May 24, 2002 - 06:05 pm: Edit
sorry bout the 2nd post.. but i noticed iodine in there... hmmm sounds like a good anti-bacterial too....sweetness... my friend is gonna add add some to a few brf substrates...in different amounts.. i will post his findings for him findings when done...also my friend is gonna try a brf powdered cake spawned into wormcasing+yeast....
By Imok Urok2 (Imok) on Saturday, May 25, 2002 - 02:33 am: Edit
Hey visions, If you want this to be moved to the contest thread just say the word. Love the pics and the tek. First, hippie invitro with chronic twist. Then, cold shock/bleach dunk tek. Now this. We live in wonderful times folks
By bill cogwell (Iphishphan) on Saturday, May 25, 2002 - 02:51 am: Edit
how much of the solution should you use to inoculate i have a few syringes i did karo style and some of them dont have as much mycelium in them as the others. Are their microscopic mycelium i dont see?
By xylem flow (Xylem) on Saturday, May 25, 2002 - 03:19 am: Edit
good question bill
i wanna know too.
By Hippie3 (Admin) on Wednesday, September 11, 2002 - 02:41 am: Edit
i just made a qt with 2 tsp of nut. yeast, we'll see what grows.
By Admin (Admin) on Tuesday, November 19, 2002 - 01:09 pm: Edit
here's mine-
Chronic007
-------------------- .
|
|