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OfflineSev
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Registered: 06/06/03
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Karo & Contams?
    #2783106 - 06/10/04 10:44 PM (12 years, 11 months ago)

Heya. A FOAF is doing his first grow using the karo tek, and is wondering just what the hell contams look like in solution. There're some small veil-y bits that he assums are shroom myc, but hasn't got a goddamned clue if they might be something else.

Does anyone know if there might be a pictorial or textual guide to IDing contams in karo on here?


--------------------
"Do we want the stars? We can have them. Can we borrow cups of fire from the sun? We can and must and light the world." --"On the Shoulders of Giants", Ray Bradbury

All of my posts are full of fiction and blatant lies.


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OfflineSuntzu
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Re: Karo & Contams? [Re: Sev]
    #2784181 - 06/11/04 10:28 AM (12 years, 11 months ago)

A few important things to check for;
1.  Colored growth on the surface of the liquid.  Obviously mold, imagine a week-old cup of coffee left to sit.
2.  Turbidity of the solution.  If the liquid looks like a little milk was added, making it not as transparent, it might have a bacterial bloom.  Good liquid mycelium cultures should not be cloudy or turbid.  Once you see the difference, it's pretty easy to tell which ones to worry about.  If possible, try swirling the solution very gently. . .often bacterial cultures will settle out to 'turbid' whereas mycelium cultures remain 'chunky' and the liquid relatively clear.  If any auto-stirring setups are used, it might be a different story. . .have never tried one.
3.  Rank smell of liquid.  Usually associated with #2.    Don't perform this test if #1 is the case.  :wink:


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OfflineCptnGarden
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Re: Karo & Contams? [Re: Sev]
    #2784719 - 06/11/04 01:55 PM (12 years, 11 months ago)

...


Edited by CptnGarden (03/29/09 01:09 AM)


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OfflineSev
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Re: Karo & Contams? [Re: Suntzu]
    #2786422 - 06/11/04 11:28 PM (12 years, 11 months ago)

Thanks, that's helpful.  :smile:

It's just odd.  There're things in most of the jars that look like whisps of thin tissue (and are usually stuck to a tiny speck of lint or hair that I missed when I cleaned out the jars before PCing them,) and then in one of my jars there's something that looks like a clump of mycelial branches.  I'm not sure which one might be a contam.  :wink:


--------------------
"Do we want the stars? We can have them. Can we borrow cups of fire from the sun? We can and must and light the world." --"On the Shoulders of Giants", Ray Bradbury

All of my posts are full of fiction and blatant lies.


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OfflineHrethic
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Re: Karo & Contams? [Re: Sev]
    #2787349 - 06/12/04 11:23 AM (12 years, 11 months ago)

I bet both are good. What seems to happen with mine is this...

After about 2 weeks if i put it with some decent heat and in a dark place, all the black spores are still visible, but there's just a white halo of fuzz around it. As it continues to grow and i continue to mix it up, the pieces start to turn into that tissue paper looking stuff, long and very stringy, and sometimes looking almost exactly like wet strainds of tissue paper. I've found that's the stuff that grows best for me: if i break the tissue paper looking stuff and suck it up into a syringe and inoc with that, it grows incredibly nice.

As to the topic of the thread... I've only had one contamed Karo batch. It wasn't actually in the karo, however, it was on the side of the tupperware container i used to store it. It honestly looked like dark green vines crawling up the side of the tupperware and all starting where the liquid stopped. I hope that was somewhat helpful. :mushroom2:


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OfflineSev
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Re: Karo & Contams? [Re: Hrethic]
    #2789043 - 06/13/04 01:53 AM (12 years, 11 months ago)

Thanks. And yeah, that is helpful.

And, boy, does that contam ever sound freaking nasty. *grin*


--------------------
"Do we want the stars? We can have them. Can we borrow cups of fire from the sun? We can and must and light the world." --"On the Shoulders of Giants", Ray Bradbury

All of my posts are full of fiction and blatant lies.


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