Home | Community | Message Board


Phytoextractum
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Contamination Forum

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Seeds Store buy cannabis seeds, Buy CBD   Amazon ½ Pint Jars, Brown Rice Flour, Microscope, Paul Stamets, Rye Grain

Jump to first unread post. Pages: 1
Invisiblezeta
Stranger

Registered: 05/25/02
Posts: 3,972
Yeast contam?
    #959143 - 10/14/02 07:12 AM (15 years, 2 months ago)

I have an occasional problem with grain that, apart from colonising poorly, has no obvious contamination, however when I smell it it smells strongly of fermentation. TMC's "Key to the common contaminants in mushroom culture" is no help.
Any ideas please?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMAIA
World-BridgerKartikeya (DftS)
Male User Gallery

Registered: 04/27/01
Posts: 7,276
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 6 months, 4 hours
Re: Yeast contam? [Re: zeta]
    #959208 - 10/14/02 08:05 AM (15 years, 2 months ago)

That smell is from yeast or bacterial contamination. Although you're not able to distinguish any color out of the ordinary, you''ll see that the grain sometimes become a bit slimy, the unpleasant odor is due to fermemtation as the alcool becomes a biproduct of the contam activity. Even if your spores germinate and try to colonize the grain you lost the battle already, it's very hard to get rid of any bacterial contam.
You should consider on reviewing your sterilization techniques, i would recomend PCing your grain for about 80-90 min. If you are using rye, try using wheat instead, i personally find rye very prone to contams.
You also could do a mycelium direct transfer. Colonize half pint of BRF jar and transfer 3 or 4 small portions (1 square cm) of colonized into the grain jar. Of course you have to do it on a glove box or something sealed. This way the mycelium takes over the grain very rapidly, leaving the probable contam with less room to grow or even germinate.

MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


Edited by MAIA (10/14/02 08:07 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblezeta
Stranger

Registered: 05/25/02
Posts: 3,972
Re: Yeast contam? [Re: MAIA]
    #959232 - 10/14/02 08:28 AM (15 years, 2 months ago)

Thanks.
It's a very rare occurrence so I'm not dying to fix it, but is there any way to identify the specific organism?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMAIA
World-BridgerKartikeya (DftS)
Male User Gallery

Registered: 04/27/01
Posts: 7,276
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 6 months, 4 hours
Re: Yeast contam? [Re: zeta]
    #959277 - 10/14/02 09:59 AM (15 years, 2 months ago)

Only with a microscope. You could try to learn something about this organisms, try this link for a start.

MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: Original Seeds Store buy cannabis seeds, Buy CBD   Amazon ½ Pint Jars, Brown Rice Flour, Microscope, Paul Stamets, Rye Grain

Mushrooms, Mycology and Psychedelics >> Contamination Forum

Similar ThreadsPosterViewsRepliesLast post
* Karo & Neutritional Yeast Contam????? repobob 2,318 8 12/09/02 08:17 AM
by repobob
* Yeast contamed straw casing thinking H2o2 dunk Gr0wer 1,107 3 06/09/04 05:25 AM
by MAIA
* yeast contam johnnyboy 747 1 11/28/08 11:33 PM
by RogerRabbit
* Half Contam'd / Half No Germinate mycmyc 695 2 09/28/07 07:37 PM
by RogerRabbit
* Yeast in my jars? johnnyboy 1,834 8 12/01/08 01:22 PM
by denger
* Yeast Contamination on wbs Jetro 2,603 3 09/24/08 10:37 PM
by Jetro
* purple contam, pink spots fast colinization clumbsy_smurf 2,094 6 10/09/02 05:15 PM
by LSAuser
* Rye jar contams. Joshua 1,675 6 01/22/02 05:49 AM
by MAIA

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Tmethyl, Pastywhyte, bodhisatta
1,397 topic views. 0 members, 3 guests and 2 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Magic-Mushrooms-Shop.com
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.003 seconds on 19 queries.