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Fridge temps to store inoculated WBS jar?
    #25510877 -

Trying to preserve colonized/colonizing WBS jars for upcoming LC usage. The issue I'm having is when I put a fully colonized jar in the fridge (not freezer) it gets dried out and doesn't appear healthy by the time I'm ready to use it. I am doing an agar run as well FYI as I realize LC isn't the best medium.

I've attempted storing colonizing jars in the fridge right after mycelial growth is visible and even 50% colonized, but the jars never finish colonizing unless I take them out into room temp.

My experienced friend said that it would colonize much slower refrigerated, so it would be healthy newer aggressive myc instead of less aggressive and dried out. Is there a certain temp range for myc to keep growing slowly? Thanks for any input.

I reside in Denver FYI (altitude).


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I'm still not good at this and it's been almost 7 years.
Shit's hard, like golf, but more... "woke."


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Re: Fridge temps to store inoculated WBS jar? [Re: Slit Sniff]
    #25510882 -

How long are you storing? I've had grain jars in the fridge for a couple of weeks or more waiting for other jars to finish with no issues, they puff back up after 24 hours back at room temp. My experience is that growth stops in a fridge, or if it is growing its extremely slow, as in no change over months. Hence I only put 100% jars in. Plus fridges are dirty too, with bacteria and mould, so you don't want uncolonised grains in them ideally

Long term I've had master jars in the range of 2 years which still produce viable growth when put back to agar

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Re: Fridge temps to store inoculated WBS jar? [Re: Shroomspective]
    #25510902 -

In reference to your colonized grain jar, I’ve never had an issue putting in in the fridge. I just break em up at spawn and it works like a charm. Once it’s spawned it should warm up fairly quickly and spring back into action. I’ve actually stored jars for months this way. Not ideal by any means, but t worked when I was in a real bind. I have my mini fridge set for one of the mid-mid high temps. I don’t have a specific number but I would say the freezer isn’t bad either as I’ve had plenty of success storing them here. Never got dried out.

Edited by FeedYourMind (10/04/18 04:13 AM)

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Re: Fridge temps to store inoculated WBS jar? [Re: Slit Sniff]
    #25513427 -

Shroomspective said:
How long are you storing? I've had grain jars in the fridge for a couple of weeks or more waiting for other jars to finish with no issues, they puff back up after 24 hours back at room temp. My experience is that growth stops in a fridge, or if it is growing its extremely slow, as in no change over months. Hence I only put 100% jars in. Plus fridges are dirty too, with bacteria and mould, so you don't want uncolonised grains in them ideally

Long term I've had master jars in the range of 2 years which still produce viable growth when put back to agar



Thanks for the input. I will stick with only refrigerating 100% jars.

Do you feel that keeping the jars covered in foil would be beneficial in/out of the fridge; making it harder for bacteria and mold to penetrate the poly-fil? I tend to stuff the poly-fil pretty tight and the holes are .25" which I hope helps with drying out


--------------------
I'm still not good at this and it's been almost 7 years.
Shit's hard, like golf, but more... "woke."


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Re: Fridge temps to store inoculated WBS jar? [Re: Slit Sniff]
    #25513502 -

I had mine with the foil in zip lock bags, leave just a tiny part open to allow some gas exchange, seems to work...pulled out a couple of jars this week to get some samples for agar, which had been sitting in there since 2016..Some metabolites at the bottom but otherwise look good as the day they went in:thumbup:

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Re: Fridge temps to store inoculated WBS jar? [Re: Slit Sniff]
    #25515218 -

Slit Sniff said:
Shroomspective said:
How long are you storing? I've had grain jars in the fridge for a couple of weeks or more waiting for other jars to finish with no issues, they puff back up after 24 hours back at room temp. My experience is that growth stops in a fridge, or if it is growing its extremely slow, as in no change over months. Hence I only put 100% jars in. Plus fridges are dirty too, with bacteria and mould, so you don't want uncolonised grains in them ideally

Long term I've had master jars in the range of 2 years which still produce viable growth when put back to agar



To answer your question I've had some jars in there for maybe 2-4 months. I use one or 2 jars every 6 weeks as an LC to inoculate 60+ jars and lately it has not gone well. The jars look 100% and solid just as they would when my grows go well, but after about a week of the bulk sub being in a tub or goes bad at ~2 weeks after spawning the bulk and jars together and put in my GH they start to show green. 1/3 of the time I still get a decent flush before the subs are taken over by mold.

This is the story of my life and the last 6 months of my grows.


--------------------
I'm still not good at this and it's been almost 7 years.
Shit's hard, like golf, but more... "woke."


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Re: Fridge temps to store inoculated WBS jar? [Re: Slit Sniff]
    #25515330 -

It seems likely that the fridge jars have contams in them, because they are fully colonised, and in the fridge its not hurting those, but it does downstream because you're using them to create LC. I only use long term jars from the fridge for starting off some agar, and then its 3+ generations of plates to make sure its clean. I'd recommend you switch from direct LC to agar as you're working with large amounts

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