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Stalled wbs jars. What to do now?
    #25503966 -

Hi everyone! Hope everyone is having a good day and thank you so much for the advice in advance. So I have quite a few wbs jars that are coming up on being a month old that have shown no sign of growth for a few weeks now. Prep was your basic straining to get all the unwanteds out and then slow cooked until 5% bursted, the only thing I can figure I did wrong with the prep was cook them to long, it didn't get mushy but I did have a few jars get pretty mushy on the bottom but I don't think that's my issue as all the jars have stalled and it was with a few different batches of bird seed. I have had issue with stalled/slow/and no growth at all with wbs. My last couple of batches did really good (except being super slow which is the norm for me:( so I thought I had it down which is why I pumped out so many this time but after this I'm getting pretty disheartened. My plan was to start working with agar to isolate so I can get faster growth and better yields but after this I'm starting to think about going back to cakes since I know I can have way more success with that and then spawning them to bulk?
Also, I shook two of them over a week ago and they have not recovered at all.

So really my question is what do I do with all these stalled jars?!?

And what are your opinions on spawning cakes to bulk?

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Re: Stalled wbs jars. What to do now? [Re: Trippies2122]
    #25504003 -

how long and what pressure did you PC them at?
Also did you incubate?
and photos? especially of the post shake.

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Re: Stalled wbs jars. What to do now? [Re: DrMushroom]
    #25504035 -

Working on uploading pictures.
Pc for 90 minutes at 15 psi
Incubation was in my SAB with a blanket, did not monitor temp

Edited by Trippies2122 (10/01/18 05:26 PM)

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Re: Stalled wbs jars. What to do now? [Re: Trippies2122]
    #25504121 -

Could have been something with the innoc?  Maybe spore syringes light, but most likely seeds too wet maybe?  Keep us updated, and yep pictures will get answers from the pros quick.

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Re: Stalled wbs jars. What to do now? [Re: PhantomDragonX]
    #25504452 -

mushy seeds dont magically become inedible, they cause issues down the road, if anything they colonize faster, at first.

what % is colonization at and how much if any standing water is in the jars. water can be overcome at a mild risk by turning jars onto their sides and rotating once a week


--------------------

Mushroom Madness for my face and the tree on the moon dinosaur hello yellow im game.

Shaman Australis Botanicals and Mycology (ethnobotany and mycology, an australia based discord) https://discord.gg/UYaH6QT

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Re: Stalled wbs jars. What to do now? [Re: DrMushroom]
    #25504467 -

I'm thinking you're prepping to wet/ dry... get some photos up, and walk us through details on prep... do not incubate, that's outdated misinformation and causes more issues than anything... top of the fridge, or shelf works great, allows for fresh GE, and ambient ~12/12 lighting... check dates on the threads you're looking at as practices evolve rather quickly and errors are often repeated from habit

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Re: Stalled wbs jars. What to do now? [Re: DrMushroom]
    #25504763 -

Jars were inoculated with multiple syringes including two prints, all showing growth so it's not the syringes. Most jars are around 15% with no standing water in any jars. I'm having issues with uploading pictures, I'll try again tomorrow when I have wifi. My grain prep is to strain to get all floaters out then very light simmer until I start seeing a couple burst kernels then strain strain strain, lay out on sheet to dry. On these particular batches I used a hair dryer to get my moister content where I wanted it then loaded jars and pc for 90 at 15. I left them over night then took them out shook and inoculated

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Re: Stalled wbs jars. What to do now? [Re: Trippies2122]
    #25504872 -

I've always been better off soaking WBS for 12-24hours and skipping any simmer/cooking, sometimes mushy and usually too wet.

After the soak, strain, mix in a small amount of verm. I also don't bother to take any "unwanteds" out unless its a stick.

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Re: Stalled wbs jars. What to do now? [Re: whosthisguy]
    #25504971 -

I would suggest you skip the WBS and use whole oats or rye berries/grain I say this because everything you did sounds right so I would assume it is a water content problem. Rye and oats are much easier to get right for a beginner. Soak for 24 hours strain and into the pc and oats are less than $15 for a 50lb bag. Your post sounds like you are asking if you can get the jars to recover and colonize. Unfortunately if they have stalled it is over for them.

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Re: Stalled wbs jars. What to do now? [Re: SHROOMSISAY01]
    #25505378 -

I soak and simmer until the milo  (larger of the 2 smallest grain, red to brown)is al denté, and pat the bottom of the strainer until it stops dripping, pc 2 hrs for quarts, 90 minutes for pints... they'll do fine between 65-75... I'd guess you're too dry :shrug: incubating is a bad idea, your jars will be a few degrees warmer from metabolic action... I'd shake the hell out of them to distribute the colonized grain throughout as evenly as possible, and shelf it ..  . If in 48 hours it's not done anything,  start over... IMO, any grain is going to have more nutrients than needed, and thus any grain is as good as any other, it's about finding a prep that works for you

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Re: Stalled wbs jars. What to do now? [Re: Caps McGee]
    #25506097 -

Caps McGee said:
I soak and simmer until the milo  (larger of the 2 smallest grain, red to brown)is al denté, and pat the bottom of the strainer until it stops dripping, pc 2 hrs for quarts, 90 minutes for pints... they'll do fine between 65-75... I'd guess you're too dry :shrug: incubating is a bad idea, your jars will be a few degrees warmer from metabolic action... I'd shake the hell out of them to distribute the colonized grain throughout as evenly as possible, and shelf it ..  . If in 48 hours it's not done anything,  start over... IMO, any grain is going to have more nutrients than needed, and thus any grain is as good as any other, it's about finding a prep that works for you



Exactly what he said!! I suggested whole oats because I think they are easier to find just about everywhere. There are throw backs to them mainly they are larger so they don't give as many inoculation points. What I would do is find out what is easiest and cheapest to get where you are. And then learn how to prepare them correctly.

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Re: Stalled wbs jars. What to do now? [Re: SHROOMSISAY01]
    #25506121 -

How big is your GE hole? Maybe things are drying up in the jars?

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Re: Stalled wbs jars. What to do now? [Re: Mycelesoul]
    #25506394 -

Teks generally call for 1/4"... I'd guess the hair dryer the culprit, the grains are still visibly moist when I load my jars... oats are suggested to be dry on the outside, but would still avoid hair dryers 

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Re: Stalled wbs jars. What to do now? [Re: Caps McGee]
    #25506697 -

Mycelesoul said:
How big is your GE hole? Maybe things are drying up in the jars?



I only have one gas exchange hole which I use to innoculat? I cover that with micropore tape... Is that not enough for gas exchange and will cause drying out?? I  have felt like my jars start to dry out,, could that be a reason for slow colonizing times also??

Caps McGee said:
Teks generally call for 1/4"... I'd guess the hair dryer the culprit, the grains are still visibly moist when I load my jars... oats are suggested to be dry on the outside, but would still avoid hair dryers 



I would agree,, that plus the long cook time didn't help me at all. I'm looking for rye berries today to try out

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Re: Stalled wbs jars. What to do now? [Re: Trippies2122]
    #25506739 -

How big is your "inoculation hole"?

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Re: Stalled wbs jars. What to do now? [Re: Caps McGee]
    #25506999 -

Caps McGee said:
How big is your "inoculation hole"?



Hahaha. It's pretty small.. maybe 1/16. Just big enough for the needle

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Re: Stalled wbs jars. What to do now? [Re: Trippies2122]
    #25507004 -

You'll want it 1/4" next run...

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Re: Stalled wbs jars. What to do now? [Re: Caps McGee]
    #25507088 -

Caps McGee said:
How big is your "inoculation hole"?



I just asked the girl next door the same question yesterday. What a coincidence. Yea it was pretty awkward.

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Re: Stalled wbs jars. What to do now? [Re: SHROOMSISAY01]
    #25507145 -

Did she let you inoculate, or give you plenty of FAE?

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Re: Stalled wbs jars. What to do now? [Re: Caps McGee] * 1
    #25507211 -

I'll just say when I left there were spores all over her.

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Re: Stalled wbs jars. What to do now? [Re: SHROOMSISAY01]
    #25507324 -

Oh my...:popcorn:
:tellmeastory:

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Re: Stalled wbs jars. What to do now? [Re: Caps McGee]
    #25507663 -



Finally able to get the pictures uploading. I think I got the answers I needed, thank you everyone for the help!!!

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Re: Stalled wbs jars. What to do now? [Re: SHROOMSISAY01]
    #25507917 -

Lmfao!


--------------------
“Silly Rabbi, Bacons for Gentiles!”

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Re: Stalled wbs jars. What to do now? [Re: Trippies2122]
    #25507927 -



you got a lot of soggy wet grains in there. what grain prep are you using?



comparison of properly hydrated milo. millet is the smallest grain and has to be hydrated more carefully. fooman hot water bath worked well for me in preventing exploded grains when i used WBS mix.


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: Stalled wbs jars. What to do now? [Re: JHOVA]
    #25507939 -

JHOVA said:


you got a lot of soggy wet grains in there. what grain prep are you using?



comparison of properly hydrated milo. millet is the smallest grain and has to be hydrated more carefully. fooman hot water bath worked well for me in preventing exploded grains when i used WBS mix.



I just soak millet for 24 hours drain for 15 mins throw them in a bag and pc for 3 hours perfect every time.

OP your jar looks like it has oatmeal in it not what you want. I think you found your problem. once done they should look very close to how they looked before you started. You want to just hydrate them not disintegrate them. See how his milo still looks like milo.

Edited by SHROOMSISAY01 (10/03/18 12:13 AM)

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Re: Stalled wbs jars. What to do now? [Re: SHROOMSISAY01]
    #25508011 -

If I were you I would soak enough bird seed to do one jar for 24 hours drain it for 15 mins.  pressure cook for 90 mins. Then inoculate with your syringe. If you don't have a syringe left to inoculate with I would wrap a fork In aluminum foil and put it in your pc with the jar. Then I would use the fork to do a G2G transfer of just the good mycelium closet to the top of a jar. And if the Jar survives you can expand with a few more G2G transfers. I think what you have is the grain is to wet to be colonized but the mycelium is still good that grew in the jars. You really have nothing to loose trying this.

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Re: Stalled wbs jars. What to do now? [Re: SHROOMSISAY01]
    #25508924 -

SHROOMSISAY01 said:
If I were you I would soak enough bird seed to do one jar for 24 hours drain it for 15 mins.  pressure cook for 90 mins. Then inoculate with your syringe. If you don't have a syringe left to inoculate with I would wrap a fork In aluminum foil and put it in your pc with the jar. Then I would use the fork to do a G2G transfer of just the good mycelium closet to the top of a jar. And if the Jar survives you can expand with a few more G2G transfers. I think what you have is the grain is to wet to be colonized but the mycelium is still good that grew in the jars. You really have nothing to loose trying this.



I am out of syringes but I just ordered more yesterday. But if that is a possibility with a good chance of the mycelium recovering I'll give it a shot rather than running through more syringes.

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Re: Stalled wbs jars. What to do now? [Re: Trippies2122]
    #25509011 -

Trippies2122 said:


Finally able to get the pictures uploading. I think I got the answers I needed, thank you everyone for the help!!!



Is that some green in the bottom of jar three in your third picture, or a trick of the light?

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Re: Stalled wbs jars. What to do now? [Re: trip_hazard]
    #25509065 -

If you used multiple syringes on those jars, you used way too much.  You dont need more than a few drops

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Re: Stalled wbs jars. What to do now? [Re: Sivarted]
    #25509185 -

Sivarted said:
If you used multiple syringes on those jars, you used way too much.  You dont need more than a few drops



I am assuming he inoculated more than the jars in the pic. In his OP he said "So I have quite a few wbs jars that are coming up on being a month old" If not that is another problem I do 1 to 2 ml per jar when I used a syringe its been a while so you might want to check that. To much spore solution will change the water content of the jar and can make it to wet.

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Re: Stalled wbs jars. What to do now? [Re: SHROOMSISAY01]
    #25509594 -

SHROOMSISAY01 said:
Sivarted said:
If you used multiple syringes on those jars, you used way too much.  You dont need more than a few drops



I am assuming he inoculated more than the jars in the pic. In his OP he said "So I have quite a few wbs jars that are coming up on being a month old" If not that is another problem I do 1 to 2 ml per jar when I used a syringe its been a while so you might want to check that. To much spore solution will change the water content of the jar and can make it to wet.



I assumed the same, but "several syringes" is too much for anything under like 50 or 60 jars (although I did assume he did fewer than that).  And using two spore prints for anything under like a couple hundred jars is massively wasteful.  (One print should make dozens of syringes)  2ml is probably too much, 1ml is unnecessary.  It really only takes a little bit, and anything beyond that is overkill (or, as you mentioned, actually harmful because of extra added water)

Of course, I also think using spores at all other than the very first time is less than ideal, so theres that.  :smile:

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Re: Stalled wbs jars. What to do now? [Re: Sivarted]
    #25509631 -

I used half of 4 syringes, about 1-2 cc each jar. I said I used multiple syringes because someone suggested a bad syringe, I was just throwing out that I didnt use the same syringe for all the jars

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Re: Stalled wbs jars. What to do now? [Re: Trippies2122]
    #25509637 -

Trippies2122 said:
I used half of 4 syringes, about 1-2 cc each jar. I said I used multiple syringes because someone suggested a bad syringe, I was just throwing out that I didnt use the same syringe for all the jars




It's already been pointed out that the most likely problem is your grain prep and them being too wet.

I was just pointing out that you dont need that much spore solution.  It really doesnt take that much, and more doesnt necessarily speed things up, either.

That's all.

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Re: Stalled wbs jars. What to do now? [Re: Sivarted]
    #25509649 -

Also, definitely start working with agar, but dont bother trying to isolate.  Clone a mushroom and clean up the culture if necessary.

For 99.99999-ish percent of people in this hobby, isolation is both pointless and potentially harmful.  (What if what you end up isolating is a strain that fruits poorly, or not at all, for example)

Spores are the slowest and least clean way to inoculate jars.

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Re: Stalled wbs jars. What to do now? [Re: Sivarted]
    #25509867 -

Sivarted said:
Also, definitely start working with agar, but dont bother trying to isolate.  Clone a mushroom and clean up the culture if necessary.

For 99.99999-ish percent of people in this hobby, isolation is both pointless and potentially harmful.  (What if what you end up isolating is a strain that fruits poorly, or not at all, for example)

Spores are the slowest and least clean way to inoculate jars.



Can you explain to me what you mean by clean up the culture? I thought isolating was cleaning it up? And making it into a single "cell" I know that's not right terminology. Isn't working with agar and isolating getting it to one true strain is what makes using agar so much faster and how people get jars colonized in 10 days and have such great canopies??

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Re: Stalled wbs jars. What to do now? [Re: Trippies2122]
    #25513368 -

Trippies2122 said:
Sivarted said:
Also, definitely start working with agar, but dont bother trying to isolate.  Clone a mushroom and clean up the culture if necessary.

For 99.99999-ish percent of people in this hobby, isolation is both pointless and potentially harmful.  (What if what you end up isolating is a strain that fruits poorly, or not at all, for example)

Spores are the slowest and least clean way to inoculate jars.



Can you explain to me what you mean by clean up the culture? I thought isolating was cleaning it up? And making it into a single "cell" I know that's not right terminology. Isn't working with agar and isolating getting it to one true strain is what makes using agar so much faster and how people get jars colonized in 10 days and have such great canopies??



Not usually, no.  Working with agar is the best way to ensure contaminant free cultures, and starting from agar is significantly faster than spores.  Spore prints and syringes are rarely 100% clean, but if a culture on agar has any contams, they show up readily on the 2d agar surface.

When cloning, it's very easy to bring contamination in with the tissue, but also usually easy to transfer uncontaminated parts to a new plate and end up with a contaminant free culture.

You have a *lot* of reading to do before even considering trying to get an isolate, starting with why and how.  (And odds are very high that you will never have reason to)

And agar as well.  There are all sorts of intros to agar around here that explain both the hoes and the whys.

Search for some.

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Re: Stalled wbs jars. What to do now? [Re: Sivarted]
    #25514085 -

"Cleaning up a culture" refers to obtaining a plate that contains ONLY the intended mycelium... I try to maintain as wide a genetic base as possible for initial tubs, then pick 5 or more fruit to clone for testing and further isolation

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