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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: The Biopsy Method: No Mercy for Bacteria. [Re: Josex]
    #25230788 -

Here are 2 overgrown dishes that I didnt even bother trying to take a transfer from. And one dish I took a transfer from but then after opening it and seeing it decided it was a lost cause and only took one little sample and moved 9n yo other dishes. If it is mesh-in mold will your Brown rice pucks help or would it just continue to eat along with the myc. Am I doomed with these and need to start over once again :frown: also idk if fermented is the proper description of smell maybe super glue or plastic model glue smell is more like the smell I'm getting out of these jars if that helps lol


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Re: The Biopsy Method: No Mercy for Bacteria. [Re: more4u2c]
    #25230997 -

It's not a clearcut case of meshed-in mold that you can tell easily, like the pics I posted before, but I'm 90% sure you've got meshed-in mold right there.


Quote:
more4u2c said:
If it is mesh-in mold will your Brown rice pucks help or would it just continue to eat along with the myc


As I told you before, it could. I had success once. Also try a hot-pour, it's also a roll of the dice but some people have been able to separate myc from mold. Try both.

Quote:
more4u2c said:
idk if fermented is the proper description of smell maybe super glue or plastic model glue smell


IME meshed-in mold doesn't give out any particular smell, are you sure what you're smelling isn't actual cube myc smell? The smell of agar cultures always reminded me a tad of the smell of glue.

Also, you mentioned earlier your wbs jars stalled out, that could be caused by grains being too dry, not by meshed-in mold.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
Has u got Magic?

PHENO HUNTERS

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Re: The Biopsy Method: No Mercy for Bacteria. [Re: Josex]
    #25231151 -

Josex said:
I've done agar MS wedge (non-bacterial) to pucks lots of times in the past just to see what'll pop up to clone, but biopsy to pucks almost always ends up being stronger looking and faster myc (I've seen bios achieving 100% colonization faster than pucks inoculated with wedges the same day) and pins more heavily on pucks most of the times.

I swear I'm not trying to sell you anything, trust me I couldn't care less and I've always hated stating things that I can't really prove or explain but it's what I've observed after having done countless pucks, the difference is huge.


I'm new to the biopsy method but I will take note that my colonization times are faster than normal, with LC's, WBS and CVG.

Cut it down a few days, as well as agreed, more aggressive and healthier tissue to the point where you can tell it's immune system isn't going to fuck up, like just a vibe.

Oh shit...I just realized I need to make more LC, prep more WBS, and spawn 3-4 tubs today.  So much for my 4 day weekend.


FYI I ran a mini-trial already comparing LC and LI from the same pucks and the LC's I am using won, majorly.  I did one tub with a combination of the two and it colonized but it's behaving oddly. (One side is rhizomorphic and the mycelium is far thicker than normal, eating my casing layer and patch jobs - not sure what the fuck)

Edited by plurfekt (05/27/18 06:34 AM)

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Re: The Biopsy Method: No Mercy for Bacteria. [Re: plurfekt]
    #25231444 -

I've had a couple of agar dished sprout a few fruits would these fruits be 100% shroom or does the meshed-in mold grow inside the fruits as well? Or could I have better success by doing a biopsy of the agar pins?

Thanks for all your help bro definitely gonna try your Pf pucks and cross my fingers

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Re: The Biopsy Method: No Mercy for Bacteria. [Re: more4u2c]
    #25231647 -

Glad to hear you're busy Plur :hehehe:

more4u2c said:
I've had a couple of agar dished sprout a few fruits would these fruits be 100% shroom or does the meshed-in mold grow inside the fruits as well? Or could I have better success by doing a biopsy of the agar pins?



That's beyond my realm of experience. The interior of the fruits should be clean but maybe not the exterior. Poking pins fails a lot, even if the pins are fat, let alone a tiny and skinny agar pin. Very often biopsies taken from pins don't take.

Quote:
Thanks for all your help bro definitely gonna try your Pf pucks and cross my fingers


Let's say your chances of success are 5% (could be more, dunno). That means that out of 20 pucks one could be successful. Doing 20 pucks may sound like a lot of work but it's really not, I make 30+ in one go every time. I'd PC a bunch of syringes and 20 or so pucks and noc em all with that culture.
Then after you do that you can pour semi-hot agar on the agar cultures you poked, just to see if that'll work.
:goodluck:


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
Has u got Magic?

PHENO HUNTERS

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Re: The Biopsy Method: No Mercy for Bacteria. [Re: Josex]
    #25235201 -

is it possible to NOT use vermiculite in your pucks so that would leave you with just brf pucks and is there any downside doing this?


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..

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Re: The Biopsy Method: No Mercy for Bacteria. [Re: pablokabute]
    #25235382 -

Never did BRF paste for this but my guess is that it won't be as effective because it doesn't have the benefit of verm to fluff it up and create gaps which hinders bacterial growth.

BRF paste looks like this:


Doesn't look like that flat and packed surface would be very effective.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
Has u got Magic?

PHENO HUNTERS

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Re: The Biopsy Method: No Mercy for Bacteria. [Re: Josex]
    #25239208 -



:standingby:


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🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: The Biopsy Method: No Mercy for Bacteria. [Re: JHOVA]
    #25239690 -

:popcorn:


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
Has u got Magic?

PHENO HUNTERS

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Re: The Biopsy Method: No Mercy for Bacteria. [Re: Josex]
    #25245500 -



Poke tomorrow.

1 GT puck was fucked. The print was taken pretty sloppily from my first grow. Luckily the other GT puck looks good and the AA pictured is real strong.

Aa

GT

Edited by JHOVA (06/03/18 05:03 PM)

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Re: The Biopsy Method: No Mercy for Bacteria. [Re: JHOVA]
    #25254165 -

:popcorn:

Saving for later


--------------------





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Re: The Biopsy Method: No Mercy for Bacteria. [Re: FishLevelMidnight]
    #25255665 -

recently im having problems germing spore syringes on agar, i oughtta give this one a try and see if i get something... ima fuckin manually crush my rice with mortar and pestle.. xD

has any of you tried this making lc also? should be perfect for the job, right?


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..

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Re: The Biopsy Method: No Mercy for Bacteria. [Re: pablokabute]
    #25255739 -

pablokabute said:
recently im having problems germing spore syringes on agar, i oughtta give this one a try and see if i get something... ima fuckin manually crush my rice with mortar and pestle.. xD

has any of you tried this making lc also? should be perfect for the job, right?


I don't know if crushing your rice is such a good idea, probably not, never did it.

Health food stores carry BRF[lour], but it's way cheaper to grind it yourself. And whatcha doing without a coffee grinder yet anyway? :lol:

And ya pucks are the bomb for germinating spore solution. If you happen to have 21G needles I'd recommend switching the thick ass needle spore syringes come with for a thinner one; more control, faster cool down and easier to squirt just the right amount. 2-3 drops per puck with a 21G needle is what I'd do.

And yes BRF is the shitz for LC, I used to do it a lot (1 gram unfiltered per 500ml of water). It makes the liquid a bit "milky" but nuttin that healthy myc won't be able to clear up eventually.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
Has u got Magic?

PHENO HUNTERS

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Re: The Biopsy Method: No Mercy for Bacteria. [Re: pablokabute]
    #25255784 -

pablokabute said:
has any of you tried this making lc also? should be perfect for the job, right?


Dunno if I understood you correctly, you meant BRF for LC? Or inoculating LC from pucks?

Inoculating LC from pucks is all I do :shrug:, haven't done agar in months and I used pucks to make LC as well as agar since I got into LC, have yet to encounter a single issue.

Just make sure you make em sterile, I sterilize them for 80 min at 20 psi, overkill alright.
Never use pucks that you used for cleaning shit and you'll be good.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
Has u got Magic?

PHENO HUNTERS

Extras: Filter Print Post Top
Re: The Biopsy Method: No Mercy for Bacteria. [Re: Josex]
    #25255849 -

Josex said:
pablokabute said:
recently im having problems germing spore syringes on agar, i oughtta give this one a try and see if i get something... ima fuckin manually crush my rice with mortar and pestle.. xD

has any of you tried this making lc also? should be perfect for the job, right?


I don't know if crushing your rice is such a good idea, probably not, never did it.

Health food stores carry BRF[lour], but it's way cheaper to grind it yourself. And whatcha doing without a coffee grinder yet anyway? :lol:

And ya pucks are the bomb for germinating spore solution. If you happen to have 21G needles I'd recommend switching the thick ass needle spore syringes come with for a thinner one; more control, faster cool down and easier to squirt just the right amount. 2-3 drops per puck with a 21G needle is what I'd do.

And yes BRF is the shitz for LC, I used to do it a lot (1 gram unfiltered per 500ml of water). It makes the liquid a bit "milky" but nuttin that healthy myc won't be able to clear up eventually.



quick and spot on replies, thank you! I shoulda just pm you but ya, this will help other readers as well, I figured.

I should definitely grab a coffee grinder when I can, lotsa uses for it too! like messing with syrian rue seeds and whatnot..

Im sorry, for your LC recipe, thats 1gram unfiltered what for 500mils of water?

Im planning to totally ditch agar now if I get comftable with this, haha


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..

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Re: The Biopsy Method: No Mercy for Bacteria. [Re: Josex]
    #25255861 -

Josex said:
pablokabute said:
has any of you tried this making lc also? should be perfect for the job, right?


Dunno if I understood you correctly, you meant BRF for LC? Or inoculating LC from pucks?

Inoculating LC from pucks is all I do :shrug:, haven't done agar in months and I used pucks to make LC as well as agar since I got into LC, have yet to encounter a single issue.

Just make sure you make em sterile, I sterilize them for 80 min at 20 psi, overkill alright.
Never use pucks that you used for cleaning shit and you'll be good.



was thinkin inoculating LC with the needle stab tissue from puck...


im not sure what you meant by this can you... uhhh.. I dunno, tell it another way?

"Never use pucks that you used for cleaning shit and you'll be good."

thanks a lot!! youve been very helpful! niiice awesome tek too,


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..

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Re: The Biopsy Method: No Mercy for Bacteria. [Re: pablokabute]
    #25255886 -

1 gram of whatever, brf, ground grains or my favorite LME.

Quote:
Im planning to totally ditch agar now if I get comftable with this, haha


Don't get me wrong I totally love agar. The reason to switch to pucks is that I've got a life and my agar game always gets crazy af, cultures grow quick and I can't seem to help transferring from every single pretty spot in a plate, it gets exponentially crazier and hard to keep up if you've got several projects.

Pucks grow slower, it's only one puck per culture at any given moment for me and I only transfer one time via poke when it gets nearly 100% colonized.

Pucks are just convenient, for badassery I'd choose agar.

Quote:
was thinkin inoculating LC with the needle stab tissue from puck...



Yeah that's what I meant, thought it was already implied.

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Re: The Biopsy Method: No Mercy for Bacteria. [Re: Josex]
    #25284513 -

this system seems very convinient and i like it, so i will deffenetly trie it...
Few moths ago my country was hard to get sterile print or surringe so what ever you get you better make at least one healty culture, so very often i had problems with contaminations...
with years i develop me own way to fight contamination chances, some where good, some where bad..
the easyest way for me is to sterilize as much 0.03 jigger, shoots, with pop corn as i can, 20, 30...
scrape the print or put the mushroom dust in to the 0.5 liter jar with water.
the way i put the spores in to the water is a bit weird:

when i boil the watter i put it to a jar just when the temp. drops arround 80 C, then i drop the dust, spores, and shake it...
if there is any contamination in spores they all get some shock, posibile death, but on the glass jar, above the water, witch is allso hot now, some dust stays sticked to....
so now i wait when everything get cold and i shake the jar again...
after few day, if watter is clear you can think that no contaminations are inside, but stil dont know if there some healthy spores are left.
so i shake the jar every day a bit, looking closely what is happening, is there any formation going on..
after a week, 10 days, you should see clearly is there some micelium starting, you can wait 2 weeks, but eventualy there some life starting...
some of micelium starts arround the dust if you put the mushroom dust instead of print...
but there are some clear micelium conections allso, you just need to take it in to your surringe now.
now when everything seems nice you inoculate as manny jiggers, shoots, of corns that you sterilized before.
some of the shoots will start go very quickly, and those one are best to start spreading after they fully colonize the jigger.
some will start easy, stop, go slowlly, but those are not good...
allso have to mention that the horse dung, pure horse dung that use to be on sun and rain at least one week, is te best substrate for micelium, i have never seen that type of dung to contaminate after sterilisig.
I am not a chemist but my exp. tells me that there is much food for micelium and like none for other cultures.
At the moment i am nursing arround 20 jars of micelium, and it all started from one healthy 0.03 jigger of corn micelium.
with one jigger i started 8 new big jars from 200-300 grams.
the first jar that reached full colonization was spread on another 12 jars, now i just dream of the next step, making more jars or spread it on a bulk, like nice dreams.
I am just giving everything a chance, and there is allways some space combination that goes well.

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Re: The Biopsy Method: No Mercy for Bacteria. [Re: huana.meri]
    #25369161 -

thank you so much for this tek great info bookmarked for sure my friend

:sporedrop:


--------------------
Spore Collection
Thai Lapi Yai 2018 end of year grow

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Re: The Biopsy Method: No Mercy for Bacteria. [Re: Josex]
    #25384710 -

Monitoring for future use.


--------------------
https://troutsnotes.com/pdf/C2_CactusCultivation.pdf
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