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Offlinemelic
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Registered: 02/21/02
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honey tek to blaim for bacteria?
    #1093295 - 11/28/02 07:28 AM (14 years, 9 months ago)

I've made a bunch of jars using two honey teks. Nr 1: put spores in a bottle with honey water and then use that solution (after a few weeks when there's a bunch of mycelium in there) and Nr 2: have honey water in a syringe and suck up some spores into it, then leave it for a week or so.

Now the jars exploded with mycelium growth when i used this stuff to innoculate, but then bacteria started growing in there too. I've gotten bacteria in some other jars also so i can't right away blaim the honey tek (well.. more like my lousy version of it, it'd rule if kept clean!), but I was hoping someone could state from experience if they have had problems with bacteria in honey water teks.

peace out


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    Look at that bunch over there man, They've spotted us !

Melic  :tongue:


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Offlinemlv
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Registered: 07/06/02
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Re: honey tek to blaim for bacteria? [Re: melic]
    #1093302 - 11/28/02 07:33 AM (14 years, 9 months ago)

using honey water can throw the moisture content off in the jars, supporting bacteria. This is the main downside I see to honey/karo water.


Edited by mlv (11/28/02 07:34 AM)


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Offlinemelic
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Re: honey tek to blaim for bacteria? [Re: mlv]
    #1093338 - 11/28/02 08:20 AM (14 years, 9 months ago)

well it should not throw off the water content since i use it instead of spore syringes, not as a complement to them


--------------------
    Look at that bunch over there man, They've spotted us !

Melic  :tongue:


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Offlinerhizo
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Re: honey tek to blaim for bacteria? [Re: melic]
    #1093385 - 11/28/02 10:37 AM (14 years, 9 months ago)

of course honey is susceptible to contams like anything else used to grow myc. I think you need to reevaluate your procedures. sounds like your jars did not get sterilized when you pc'd/boiled, as you said other jars got contams which you didn't shoot with honey.


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An optimist is never pleasantly surprised.


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OfflineMAIA
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Re: honey tek to blaim for bacteria? [Re: melic]
    #1093562 - 11/28/02 01:21 PM (14 years, 9 months ago)

How did you sterilize the honey water ?

MAIA


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Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
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Offlinemelic
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Re: honey tek to blaim for bacteria? [Re: MAIA]
    #1093752 - 11/28/02 02:26 PM (14 years, 9 months ago)

I think i bought sterile water.
i think..
it's possible i used boiled tap water though.
either way i then boiled the bottle.
i didn't have a PC at the time


--------------------
    Look at that bunch over there man, They've spotted us !

Melic  :tongue:


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OfflineMAIA
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Re: honey tek to blaim for bacteria? [Re: melic]
    #1095509 - 11/29/02 06:14 AM (14 years, 9 months ago)

When did you add the honey to the water ? Before or after sterilizing ?
Anyway, never mind, boiling water is not enough to sterilize it, you have to PC it, or another technique i used with success is microwaving the honey water, 5 to 8 minutes on max power. To do this i used a jar with a plastic lid and one hole poked on lid and taped. After cooling down, it's innocualted with a spore syringe.
Honey water contams very easeally, you've got to be super sterile. Better luck next time ....

MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


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Amazon Shop: ½ Pint Jars

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